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Full-Text Articles in Life Sciences
Implication Of The Mir-184 And Mir-204 Competitive Rna Network In Control Of Mouse Secondary Cataract, Andrea Hoffmann, Yusen Huang, Rinako Suetsugu-Maki, Carol S. Ringelberg, Craig R. Tomlinson, Katia Del Rio-Tsonis, Panagiotis A. Tsonis
Implication Of The Mir-184 And Mir-204 Competitive Rna Network In Control Of Mouse Secondary Cataract, Andrea Hoffmann, Yusen Huang, Rinako Suetsugu-Maki, Carol S. Ringelberg, Craig R. Tomlinson, Katia Del Rio-Tsonis, Panagiotis A. Tsonis
Biology Faculty Publications
The high recurrence rate of secondary cataract (SC) is caused by the intrinsic differentiation activity of residual lens epithelial cells after extra-capsular lens removal. The objective of this study was to identify changes in the microRNA (miRNA) expression profile during mouse SC formation and to selectively manipulate miRNA expression for potential therapeutic intervention. To model SC, mouse cataract surgery was performed and temporal changes in the miRNA expression pattern were determined by microarray analysis. To study the potential SC counterregulative effect of miRNAs, a lens capsular bag in vitro model was used. Within the first 3 wks after cataract surgery, …
A Complement Receptor C5a Antagonist Regulates Epithelial To Mesenchymal Transition And Crystallin Expression After Lens Cataract Surgery In Mice, Rinako Suetsugu-Maki, Nobuyasu Maki, Timothy P. Fox, Kenta Nakamura, Richard Cowper.Solari, Craig R. Tomlinson, Hongchang Qu, John D. Lambris, Panagiotis A. Tsonis
A Complement Receptor C5a Antagonist Regulates Epithelial To Mesenchymal Transition And Crystallin Expression After Lens Cataract Surgery In Mice, Rinako Suetsugu-Maki, Nobuyasu Maki, Timothy P. Fox, Kenta Nakamura, Richard Cowper.Solari, Craig R. Tomlinson, Hongchang Qu, John D. Lambris, Panagiotis A. Tsonis
Biology Faculty Publications
Purpose: To evaluate the effects of complement employing a mouse model for secondary cataract. Methods: The role of complement receptor C5a (CD88) was evaluated after cataract surgery in mice. An antagonist specific to C5a receptor was administered intraperitoneally to mice. Epithelial to mesenchymal transition (EMT) was evaluated by alpha-smooth muscle actin (α-SMA) staining and proliferation by bromodeoxyuridine (5-bromo-2'- deoxyuridine, BrdU) incorporation. Gene expression patterns was examined by microarray analysis and quantitative polymerase chain reaction (QPCR). Results: We found that administration of a C5aR antagonist in C57BL/6J mice decreases EMT, as evidenced by α-SMA expression, and cell proliferation. Gene expression by …
Gene Expression And Discovery During Lens Regeneration In Mouse: Regulation Of Epithelial To Mesenchymal Transition And Lens Differentiation, Mario Medvedovic, Craig R. Tomlinson, Mindy Kay Call, Matthew Grogg, Panagiotis A. Tsonis
Gene Expression And Discovery During Lens Regeneration In Mouse: Regulation Of Epithelial To Mesenchymal Transition And Lens Differentiation, Mario Medvedovic, Craig R. Tomlinson, Mindy Kay Call, Matthew Grogg, Panagiotis A. Tsonis
Biology Faculty Publications
Purpose: It has been shown that after extracapsular lens removal by anterior capsulotomy in the mouse, the lens can be regenerated. However, as the capsular bag is filled with fibers, epithelial to mesenchymal transition (EMT), an event which is common after cataract surgery as well, takes place during early stages. This study, using a unique mouse model, was undertaken to identify novel regulators and networks in order to more clearly understand secondary cataracts at the molecular level.