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Full-Text Articles in Life Sciences
A Microarray Analysis Of Gene Expression Patterns During Early Phases Of Newt Lens Regeneration, Konstantinos Sousounis, Christian S. Michel, Marc Bruckskotten, Nobuyasu Maki, Thilo Borchardt, Thomas Braun, Mario Looso, Panagiotis A. Tsonis
A Microarray Analysis Of Gene Expression Patterns During Early Phases Of Newt Lens Regeneration, Konstantinos Sousounis, Christian S. Michel, Marc Bruckskotten, Nobuyasu Maki, Thilo Borchardt, Thomas Braun, Mario Looso, Panagiotis A. Tsonis
Biology Faculty Publications
Purpose: Notophthalmus viridescens, the red-spotted newt, possesses tremendous regenerative capabilities. Among the tissues and organs newts can regenerate, the lens is regenerated via transdifferentiation of the pigment epithelial cells of the dorsal iris, following complete removal (lentectomy). Under normal conditions, the same cells from the ventral iris are not capable of regenerating. This study aims to further understand the initial signals of lens regeneration.
A Complement Receptor C5a Antagonist Regulates Epithelial To Mesenchymal Transition And Crystallin Expression After Lens Cataract Surgery In Mice, Rinako Suetsugu-Maki, Nobuyasu Maki, Timothy P. Fox, Kenta Nakamura, Richard Cowper.Solari, Craig R. Tomlinson, Hongchang Qu, John D. Lambris, Panagiotis A. Tsonis
A Complement Receptor C5a Antagonist Regulates Epithelial To Mesenchymal Transition And Crystallin Expression After Lens Cataract Surgery In Mice, Rinako Suetsugu-Maki, Nobuyasu Maki, Timothy P. Fox, Kenta Nakamura, Richard Cowper.Solari, Craig R. Tomlinson, Hongchang Qu, John D. Lambris, Panagiotis A. Tsonis
Biology Faculty Publications
Purpose: To evaluate the effects of complement employing a mouse model for secondary cataract. Methods: The role of complement receptor C5a (CD88) was evaluated after cataract surgery in mice. An antagonist specific to C5a receptor was administered intraperitoneally to mice. Epithelial to mesenchymal transition (EMT) was evaluated by alpha-smooth muscle actin (α-SMA) staining and proliferation by bromodeoxyuridine (5-bromo-2'- deoxyuridine, BrdU) incorporation. Gene expression patterns was examined by microarray analysis and quantitative polymerase chain reaction (QPCR). Results: We found that administration of a C5aR antagonist in C57BL/6J mice decreases EMT, as evidenced by α-SMA expression, and cell proliferation. Gene expression by …
Expression Profiles During Dedifferentiation In Newt Lens Regeneration Revealed By Expressed Sequence Tags, Nobuyasu Maki, John Martinsou, Osamu Nishimura, Hiroshi Tarui, Jaroslaw Meller, Panagiotis A. Tsonis, Kiyokazu Agata
Expression Profiles During Dedifferentiation In Newt Lens Regeneration Revealed By Expressed Sequence Tags, Nobuyasu Maki, John Martinsou, Osamu Nishimura, Hiroshi Tarui, Jaroslaw Meller, Panagiotis A. Tsonis, Kiyokazu Agata
Biology Faculty Publications
Purpose: The adult newt can regenerate lens from pigmented epithelial cells (PECs) of the dorsal iris via dedifferentiation. The purpose of this research is to obtain sequence resources for a newt lens regeneration study and to obtain insights of dedifferentiation at the molecular level.
Regeneration Of Retinotectal Projections After Optic Tectum Removal In Adult Newts, Mitsumasa Okamoto, Hatsuki Ohsawa, Toshinori Hayashi, Katsushi Owaribe, Panagiotis A. Tsonis
Regeneration Of Retinotectal Projections After Optic Tectum Removal In Adult Newts, Mitsumasa Okamoto, Hatsuki Ohsawa, Toshinori Hayashi, Katsushi Owaribe, Panagiotis A. Tsonis
Biology Faculty Publications
Purpose: When injured, the adult newt possesses the remarkable capability to regenerate tissues and organs with return of function and physiology. One example is the newt eye, in which regeneration can restore normal vision if the retina or lens has been removed. We wanted to examine how the retinotectal projections regenerate after removal of the brain’s optic tectum and establish this animal as a model for retinal projection as well as a central nervous system regeneration model.
Identification Of Micrornas And Other Small Rnas From The Adult Newt Eye, Evgeny Makarev, Jason R. Spence, Katia Del Rio-Tsonis, Panagiotis A. Tsonis
Identification Of Micrornas And Other Small Rnas From The Adult Newt Eye, Evgeny Makarev, Jason R. Spence, Katia Del Rio-Tsonis, Panagiotis A. Tsonis
Biology Faculty Publications
MicroRNAs (miRNAs) are capable of controlling gene expression by targeting complimentary sequences in many mRNAs. Thus, a small number of miRNAs are capable of regulating expression of many different genes. miRNAs have been found in all animals from Drosophila to human and they are highly conserved. This work was undertaken in order to identify such RNAs in the newt eye.
Gene Expression And Discovery During Lens Regeneration In Mouse: Regulation Of Epithelial To Mesenchymal Transition And Lens Differentiation, Mario Medvedovic, Craig R. Tomlinson, Mindy Kay Call, Matthew Grogg, Panagiotis A. Tsonis
Gene Expression And Discovery During Lens Regeneration In Mouse: Regulation Of Epithelial To Mesenchymal Transition And Lens Differentiation, Mario Medvedovic, Craig R. Tomlinson, Mindy Kay Call, Matthew Grogg, Panagiotis A. Tsonis
Biology Faculty Publications
Purpose: It has been shown that after extracapsular lens removal by anterior capsulotomy in the mouse, the lens can be regenerated. However, as the capsular bag is filled with fibers, epithelial to mesenchymal transition (EMT), an event which is common after cataract surgery as well, takes place during early stages. This study, using a unique mouse model, was undertaken to identify novel regulators and networks in order to more clearly understand secondary cataracts at the molecular level.