Life Sciences Commons^™

Potential Anticancer Effect Of Prostratin Through Sik3 Inhibition, Dalal Alotaibi, Suneetha Amara, Terrance L. Johnson, Venkataswarup Tiriveedhi

Biology Faculty Research

Prostratin, a phorbol ester natural plant compound, has been demonstrated to exert an anti‑retroviral effect through activation of latent cluster of differentiation (CD)4+T lymphocytes and inhibition of viral entry into the cell through downregulation of chemokine receptor type 4 (CXCR4) expression. However, the potential effect of prostratin on cancer is yet to be defined. As CXCR4 is well known to induce cancer migration, it was hypothesized that prostratin induces an anti‑cancer effect through inhibition of CXCR4 expression. The authors previously demonstrated that high stimulating conditions (sub‑minimal IL‑17, 0.1 ng/ml, synergized with high salt, Δ0.05 M NaCl) promote breast cancer cell …

A Study On Organic Tomatoes: Effect Of A Biostimulator On Phytochemical And Antioxidant Activities, Varinder Sidhu, Dilip Nandwani, Li Wang, Ying Wu

Agricultural and Environmental Sciences Faculty Research

The objective of the present study is to investigate nutritional and antioxidant activity of four types of organic tomato cultivars. The differences in tomato quality are also tested between groups with or without treatment using an organic biostimulator, Stimplex. Total phenolic compounds (TPC), lycopene, 𝛽-carotene, DPPH free radical scavenging activity, reducing power, and color parameters were investigated in the current study. The results showed that there was no significant difference in TPC among cultivars regardless of Stimplex treatment. Higher lycopene and 𝛽-carotene were obtained in Stimplex treated tomatoes. Lycopene and -carotene contents were significantly different among cultivars (𝑃<0.05). DPPH scavenging activity in controlled group was significantly higher than that in the Stimplex treated tomatoes (𝑃<0.05). No significant difference in reducing power was detected among cultivars treatment groups. The study showed that the darker the tomato color, the higher the lycopene and 𝛽-carotene contents and the stronger the reducing power.

Morphological Variation And Inter-Relationships Of Quantitative Traits In Enset (Ensete Ventricosum (Welw.) Cheesman) Germplasm From South And South-Western Ethiopia, Zerihun Yemataw, Alemayehu Chala, Daniel Ambachew, David J. Studholme, Murray R. Grant, Kassahun Tesfaye

Agricultural and Environmental Sciences Student Research

Enset (Ensete ventricosum (Welw.) Cheesman) is Ethiopia’s most important root crop. A total of 387 accessions collected from nine different regions of Ethiopia were evaluated for 15 quantitative traits at Areka Agricultural Research Centre to determine the extent and pattern of distribution of morphological variation. The variations among the accessions and regions were significant (p ≤ 0.01) for all the 15 traits studied. Mean for plant height, central shoot weight before grating, and fermented squeezed kocho yield per hectare per year showed regional variation along an altitude gradient and across cultural differences related to the origin of the collection. Furthermore, …

Complete Genome Sequence Of A New Isolate Of Solenopsis Invictavirus 3 From Solenopsis Invicta × Richteri Hybrid Ants, Steven M. Valles, Jason B. Oliver, Karla M. Addesso

Agricultural and Environmental Sciences Faculty Research

Solenopsis invicta virus 3 (SINV-3) is a positive-sense, single-stranded RNA virus that infects the red imported fire ant, Solenopsis invicta Buren. We report here the full genome (10,383 nucleotides) of an isolate infecting Solenopsis invicta× richteri hybrid ants, which we have identified as SINV-3 hybrid.

Diversity In Grain Amaranths And Relatives Distinguished By Genotyping By Sequencing (Gbs), Xingbo Wu, Matthew W. Blair

Agricultural and Environmental Sciences Faculty Research

The genotyping by sequencing (GBS) method has become a molecular marker technology of choice for many crop plants because of its simultaneous discovery and evaluation of a large number of single nucleotide polymorphisms (SNPs) and utility for germplasm characterization. Genome representation and complexity reduction are the basis for GBS fingerprinting and can vary by species based on genome size and other sequence characteristics. Grain amaranths are a set of three species that were domesticated in the New World to be high protein, pseudo-cereal grain crops. The goal of this research was to employ the GBS technique for diversity evaluation in …

Detecting Large Deletions At Base Pair Level By Combining Split Read And Paired Read Data, Matthew Hayes, Jeremy S. Pearson

Computer Science Student Research

Background

Genomic structural variants (SV) play a significant role in the onset and progression of cancer. Genomic deletions can create oncogenic fusion genes or cause the loss of tumor suppressing gene function which can lead to tumorigenesis by downregulating these genes. Detecting these variants has clinical importance in the treatment of diseases. Furthermore, it is also clinically important to detect their breakpoint boundaries at high resolution. We have generalized the framework of a previously-published algorithm that located translocations, and we have applied that framework to develop a method to locate deletions at base pair level using next-generation sequencing data. Our …

Microbial Inactivation And Cytotoxicity Evaluation Of Uv Irradiated Coconut Water In A Novel Continuous Flow Spiral Reactor, Manreet Singh Bhullar, Ankit Patras, Agnes Kilonzo-Nthenge, Bharat Pokharel, Sudheer K. Yannam, Kanyasiri Rakariyatham, Che Pan, Hang Xiao, Michael Sasges

Agricultural and Environmental Sciences Faculty Research

A continuous-flow UV reactor operating at 254 nm wave-length was used to investigate inactivation of microorganisms including bacteriophage in coconut water, a highly opaque liquid food. UV-C inactivation kinetics of two surrogate viruses (MS2, T1UV) and three bacteria (E. coli ATCC 25922, Salmonella Typhimurium ATCC 13311, Listeria monocytogenes ATCC 19115) in buffer and coconut water were investigated (D₁₀ values ranging from 2.82 to 4.54 mJ·cm^{− 2}). A series of known UV-C doses were delivered to the samples. Inactivation levels of all organisms were linearly proportional to UV-C dose (r² > 0.97). At the highest dose …

Stability Of Cacopsylla Pyricola (Hemiptera: Psyllidae) Populations In Pacific Northwest Pear Orchards Managed With Long-Term Mating Disruption For Cydia Pomonella (Lepidoptera: Tortricidae), Kaushalya G. Amarasekare, Peter W. Shearer

Agricultural and Environmental Sciences Faculty Research

This study focused on conservation biological control of pear psylla, Cacopsylla pyricola, in the Pacific Northwest, USA. We hypothesized that insecticides applied against the primary insect pest, codling moth Cydia pomonella, negatively impact natural enemies of pear psylla, thus causing outbreaks of this secondary pest. Hence, the objective of this study was to understand how codling moth management influences the abundance of pear psylla and its natural enemy complex in pear orchards managed under long-term codling moth mating disruption programs. We conducted this study within a pear orchard that had previously been under seasonal mating disruption for codling moth for …

Efficacy Of Ultraviolet (Uv-C) Light In Reducing Foodborne Pathogens And Model Viruses In Skim Milk, Danielle M. Gunter-Ward, Ankit Patras, Manreet Singh Bhullar, Agnes Kilonzo-Nthenge, Bharat Pokharel, Michael Sasges

Agricultural and Environmental Sciences Faculty Research

The efficacy of low wavelength ultraviolet light (UV-C) as a disinfection process for a scattering fluid such as skim milk was investigated in this study. UV-C inactivation kinetics of two surrogate viruses (bacteriophages MS2 and T1UV) and three bacteria Escherichia coli ATCC 25922, S. Typhimurium ATCC 13311, Listeria monocytogenes ATCC 19115 in buffer and skim milk were investigated. UV-C irradiation was applied to stirred samples, using a collimated beam operating at 253.7 nm wavelength. A series of known UV-C doses (0–40 mJ·cm⁻²) were delivered to the samples except MS2 where higher doses (0–150 mJ·cm⁻²) were delivered. …

Genome Structure Of Bacillus Cereus Tsu1 And Genes Involved In Cellulose Degradation And Poly-3-Hydroxybutyrate Synthesis, Hui Li, Suping Zhou, Terrance Johnson, Koen Vercruysse, Lizhi Ouyang, Ranganathan Parthasarathy, Nsoki Phambu, Alexander J. Ropelewski, Theodore W. Thannhauser

Chemistry Faculty Research

In previous work, we reported on the isolation and genome sequence analysis of Bacillus cereus strain tsu1 NCBI accession number JPYN00000000. The 36 scaffolds in the assembled tsu1 genome were all aligned with B. cereus B4264 genome with variations. Genes encoding for xylanase and cellulase and the cluster of genes in the poly-3-hydroxybutyrate (PHB) biosynthesis pathway were identified in tsu1 genome. The PHB accumulation in B. cereus tsu1 was initially identified using Sudan Black staining and then confirmed using high-performance liquid chromatography. Physical properties of these PHB extracts, when analyzed with Raman spectra and Fourier transform infrared spectroscopy, were found …

Identification Of Hydrated And Dehydrated Lipids And Protein Secondary Structures In Seeds Of Cotton (Gossypium Hirsutum) Line, Diwas Kumar Silwal, Nsoki Phambu, Bharat Pokharel, Ahmad Naseer Aziz

Chemistry Faculty Research

Cottonseeds from two parents (TM-1 and 3-79) and their 17 progeny (chromosomal substitution) lines were analyzed for various secondary structures of proteins and moisture content of lipids, separately in hulls and kernels. Fourier transform infrared spectroscopy (FTIR) was used on mature seeds from Upland cotton (G. hirsutum) progeny lines and parents. Based on secondary structures of proteins and hydration levels of lipids, differences were observed among the cottonseeds. The two progeny lines – CS-B12sh and CS-B22sh retained lipid moisture content and protein secondary structures similar to both parents, while CS-B06, CS-B15sh and CS-B16 remained distinct from either parent. On the …

Butyltin Compounds Alter Secretion Of Interleukin 6 From Human Immune Cells, Shyretha Brown, Wendy Wilburn, Tyesha Martin, Margaret Whalen

Chemistry Faculty Research

Butyltins (BTs), tributyltin (TBT) and dibutyltin (DBT) are organotin compounds that have been used in a variety of industrial applications; as a result, these compounds have been found in human blood. Interleukin (IL)-6 is a proinflammatory mediator that is produced by T lymphocytes and monocytes. It is responsible for immune response regulation as well as tissue repair and cellular growth. Both BTs decrease the ability of human natural killer cells to destroy tumor cells and alter the secretion of proinflammatory cytokines tumor necrosis factor alpha, interferon gamma and IL-1 beta (β) from human lymphocytes ex vivo. Here, we show that …

Availability Of Low-Fat Milk And Produce In Small And Mid-Sized Grocery Stores After 2014 Wic Final Rule Changes, Tennessee, David Schlundt, Chiquita Briley, Barbara Canada, Jessica L. Jones, Baqar A. Husaini, Janice S. Emerson, Pamela C. Hull

Center for Prevention Research Publications

Introduction

The 2007 Interim Rule mandated changes to food packages in the Special Supplemental Nutrition Program for Women, Infants, and Children (WIC) for implementation by 2009. The 2014 Final Rule required additional changes, including increasing the cash value voucher for fruits and vegetables from $6 to $8 for children by June 2014, and allowing only low-fat (1%) or nonfat milk for mothers and children aged 2 to 4 years by October 2014. This study evaluated the effect of the 2014 Final Rule changes on the food environment of small and mid-sized WIC-authorized grocery stores.

Methods

We analyzed secondary data using …

Yield Performance Of Organic Sweetpotato Varieties In Various Mulches, Sochinwechi Nwosisi, Dilip Nandwani, Bharat Pokharel

Agricultural and Environmental Sciences Faculty Research

Interest in organic sweetpotato production in the United States has been growing as consumers and producers are becoming increasingly concerned about how their food is cultivated. Thus, there is a growing need for information on sweetpotato production and variety selection under an organic management production system. The objective of this study was to evaluate the effects of various mulches on yields—total and marketable—of fourteen sweetpotato varieties grown under organic management. Three types of mulch, wheat straw, pine needle, and black plastic, and a control (no mulch) were evaluated during the 2016 growing season. The wheat straw mulch yielded significantly higher …

Transcriptional Analysis Of Abdominal Fat In Chickens Divergently Selected On Bodyweight At Two Ages Reveals Novel Mechanisms Controlling Adiposity: Validating Visceral Adipose Tissue As A Dynamic Endocrine And Metabolic Organ, Christopher W. Resnyk, Wilfrid Carré, Xiaofei Wang, Tom E. Porter, J. Simon, Elisabeth Le Bihan-Duval, Michel J. Duclos, Sammy E. Aggrey, Larry A. Cogburn

Biology Faculty Research

Background

Decades of intensive genetic selection in the domestic chicken (Gallus gallus domesticus) have enabled the remarkable rapid growth of today’s broiler (meat-type) chickens. However, this enhanced growth rate was accompanied by several unfavorable traits (i.e., increased visceral fatness, leg weakness, and disorders of metabolism and reproduction). The present descriptive analysis of the abdominal fat transcriptome aimed to identify functional genes and biological pathways that likely contribute to an extreme difference in visceral fatness of divergently selected broiler chickens.

Methods

We used the Del-Mar 14 K Chicken Integrated Systems microarray to take time-course snapshots of global gene transcription …

Effects Of Precipitation Changes On Aboveground Net Primary Production And Soil Respiration In A Switchgrass Field, Qi Deng, Sadiye Aras, Chih-Li Yu, Kudjo E. Dzantor, Philip A. Fay, Yiqi Luo, Weijun Shen, Dafeng Hui

Biology Faculty Research

Switchgrass (Panicum virgatum L.) is widely selected as a model feedstock for sustainable replacement of fossil fuels and climate change mitigation. However, how climate changes, such as altered precipitation (PPT), will influence switchgrass growth and soil carbon storage potential have not been well investigated. We conducted a two-year PPT manipulation experiment with five treatments: −50%, −33%, +0%, +33%, and +50% of ambient PPT, in an “Alamo” switchgrass field in Nashville, TN. Switchgrass aboveground net primary production (ANPP), leaf gas exchange, and soil respiration (SR) were determined each growing season. Data collected from this study was then used to test whether …

Social Context-Dependent Activity In Marmoset Frontal Cortex Populations During Natural Conversations, Samuel U. Nummela, Vladimir Jovanovic, Lisa De La Mothe, Cory T. Miller

Psychology Faculty Research

Communication is an inherently interactive process that weaves together the fabric of both human and nonhuman primate societies. To investigate the properties of the primate brain during active social signaling, we recorded the responses of frontal cortex neurons as freely moving marmosets engaged in conversational exchanges with a visually occluded virtual marmoset. We found that small changes in firing rate (∼1 Hz) occurred across a broadly distributed population of frontal cortex neurons when marmosets heard a conspecific vocalization, and that these changes corresponded to subjects' likelihood of producing or withholding a vocal reply. Although the contributions of individual neurons were …

Rain-Induced Changes In Soil Co2 Flux And Microbial Community Composition In A Tropical Forest Of China, Qi Deng, Dafeng Hui, Guowei Chu, Xi Han, Quanfa Zhang

Biology Faculty Research

Rain-induced soil CO₂ pulse, a rapid excitation in soil CO₂ flux after rain, is ubiquitously observed in terrestrial ecosystems, yet the underlying mechanisms in tropical forests are still not clear. We conducted a rain simulation experiment to quantify rain-induced changes in soil CO₂ flux and microbial community composition in a tropical forest. Soil CO₂ flux rapidly increased by ~83% after rains, accompanied by increases in both bacterial (~51%) and fungal (~58%) Phospholipid Fatty Acids (PLFA) biomass. However, soil CO₂ flux and microbial community in the plots without litters showed limited response to rains. Direct releases …

Draft Genome Sequence Of Bacillus Altitudinis Ynp4-Tsu, Isolated From Yellowstone National Park, Joshua A. O'Hair, Hui Li, Santosh Thapa, Matthew Scholz, Suping Zhou

Agricultural and Environmental Sciences Faculty Research

Undisturbed hot springs inside Yellowstone National Park remain a dynamic biome for novel cellulolytic thermophiles. We report here the draft genome sequence of one of these isolates, Bacillus altitudinis YNP4-TSU.

Critical Role Of Sik3 In Mediating High Salt And Il-17 Synergy Leading To Breast Cancer Cell Proliferation, Suneetha Amara, Ciera Majors, Bipradas Roy, Salisha Hill, Kristie L. Rose, Elbert L. Myles, Venkataswarup Tiriveedhi

Biology Faculty Research

Chronic inflammation is a well-known precursor for cancer development and proliferation. We have recently demonstrated that high salt (NaCl) synergizes with sub-effective interleukin (IL)-17 to induce breast cancer cell proliferation. However, the exact molecular mechanisms mediating this effect are unclear. In our current study, we adopted a phosphoproteomic-based approach to identify salt modulated kinase-proteome specific molecular targets. The phosphoprotemics based binary comparison between heavy labelled MCF-7 cells treated with high salt (Δ0.05 M NaCl) and light labelled MCF-7 cells cultured under basal conditions demonstrated an enhanced phosphorylation of Serine-493 of SIK3 protein. The mRNA transcript and protein expression analysis of …

The Longhorned Beetles (Coleoptera: Cerambycidae) Of Tennessee: Distribution Of Species, Seasonal Adult Activity, And New State Records, William E. Klingeman, Nadeer N. Youssef, Jason B. Oliver, Joshua P. Basham

Agricultural and Environmental Sciences Faculty Research

Efforts to document species of longhorned beetles (Coleoptera: Cerambycidae) occurring in Tennessee have not been updated since 1973. To address this knowledge gap, institutional, research, and private collections in Tennessee were reviewed to provide faunal distribution assessments and seasonal activity data for the cerambycid beetle species active in Tennessee. Examinations of 9,918 specimens and records yielded a list of 230 cerambycid beetle species within 5 subfamilies. Twenty-seven species are reported as new state records from Tennessee. Adult seasonal activity data that were recorded on specimen labels are presented. Where available, notes on collection method, adult resources, and larval host plants …

Ginseng And Obesity: Observations And Understanding In Cultured Cells, Animals And Humans, Longyun Zhang, Carlos Virgous, Hongwei Si

Human Sciences Faculty Research

Ginseng, a traditional medical herb, has been reported having beneficial effects in fatigue, heart diseases, diabetes, immune function and erectile dysfunction. In recent years, increasing investigations have been conducted on ginseng in preventing and treating of obesity, one of the major worldwide escalating public health concerns. However, the effect and the relevant mechanisms behind how ginseng works as an antiobesity treatment are still controversial. In this review, we briefly discussed the chemical structures, metabolism and pharmacokinetics of ginseng and its major bioactive components ginsenosides. The major focus is on the antiobesity effects and the physiological, cellular and molecular mechanisms of …

Chain Length Effect On The Structure And Stability Of Antimicrobial Peptides Of The (Rw)N Series, Nsoki Phambu, Bashiyar Almarwani, Arlette M. Garcia, Nafisa S. Hamza, Amira Muhsen, Jacqueline E. Baidoo, Anderson Sunda-Meya

Chemistry Faculty Research

Three peptides containing (RW)n-NH2 units (where n=4, 6, and 8) have been chosen to study the effect of the chain length on the structure and stability of the peptide using Fourier transform infrared (FTIR), scanning electron microscopy (SEM), thermogravimetric analysis (TGA), and differential scanning calorimetry (DSC) techniques. Their interactions with Escherichia coli (E. coli) membrane mimetic vesicles are discussed. Infrared results indicate that addition of (RW)n-NH2 units increases intermolecular H bonds with antiparallel orientation. TGA and DSC results reveal that (RW)6-NH2 shows the optimal chain length in terms of stability and all three peptides show a preferential interaction with one …

Trait Associations In Diversity Panels Of The Two Common Bean (Phaseolus Vulgaris L.) Gene Pools Grown Under Well-Watered And Water-Stress Conditions, Asrat Asfaw, Daniel Ambachew, Trushar Shah, Matthew W. Blair

Agricultural and Environmental Sciences Faculty Research

Common beans are a warm-season, food legume cultivated in areas prone to water limitation throughout their growing season. This study assessed the magnitude and pattern of trait associations for a total of 202 common bean genotypes divided into panels of 81 Andean and 121 Mesoamerican gene pool accessions grown under contrasting treatments of well-watered, non-stress, and water-limited, terminal drought-stress conditions. Linear correlation, complex path coefficient, and genetic divergence analyses were used to dissect the relationship dynamics between traits and the relative contribution of adaptive traits to differentiation among gene pools and genotypes based on drought stress. Drought severity level for …

Modeling Net Energy Balance Of Ethanol Production From Native Warm Season Grasses, Prabodh Illukpitiya, Chandra Reddy, Ankit Bansal

Agricultural and Environmental Sciences Faculty Research

There has been an increasing interest in the use of perennial grasses as potential feedstock for ethanol production. The characteristics which make perennial grasses attractive for bioenergy feedstock development initiative are their high yield potential and the high contents of lignin and cellulose. The objective of the study is to model energy input and output and simulate Net Energy Value (NEV) of producing ethanol from native warm season grasses. According to simulated results, the mean NEV of ethanol production from native warm season grasses considered in the analysis was positive. Mean NEV for switchgrass and eastern gammagrass was higher compared …

Effect Of Non-Nutritive Sugars To Decrease The Survivorship Of Spotted Wing Drosophila, Drosophila Suzukii, Man-Yeon Choi, Siew Bee Tang, Seung-Joon Ahn, Kaushalya G. Amarasekare, Peter Shearer, Jana C. Lee

Agricultural and Environmental Sciences Faculty Research

In this study, we investigated the effects of non-nutritive sugars and sugar alcohols on the survivorship of spotted wing drosophila, Drosophila suzukii, and found erythritol and erythrose as potentially insecticidal to the fly. In a dose-dependent study, erythritol and erythrose significantly reduced fly longevity, with 100% mortality with 1, 0.5, 0.1 & 0.05 M doses after feeding for 7 days. When sucrose and erythritol solutions were provided separately to flies for 7 days, there was no effect on survivorship regardless of erythritol concentrations. However, with a serial combination of sucrose and erythritol solutions, fly survivorship was significantly decreased for the …

Avian And Mammalian Facilitative Glucose Transporters, Mary Shannon Byers, Christianna Howard, Xiaofei Wang

Biology Faculty Research

The GLUT members belong to a family of glucose transporter proteins that facilitate glucose transport across the cell membrane. The mammalian GLUT family consists of thirteen members (GLUTs 1–12 and H+-myo-inositol transporter (HMIT)). Humans have a recently duplicated GLUT member, GLUT14. Avians express the majority of GLUT members. The arrangement of multiple GLUTs across all somatic tissues signifies the important role of glucose across all organisms. Defects in glucose transport have been linked to metabolic disorders, insulin resistance and diabetes. Despite the essential importance of these transporters, our knowledge regarding GLUT members in avians is fragmented. It is clear that …

Nonlinear Responses Of Soil Respiration To Precipitation Changes In A Semiarid Temperate Steppe, Yuan Miao, Hongyan Han, Yue Du, Qian Zhang, Lin Jiang, Dafeng Hui, Shiqiang Wan

Biology Faculty Research

Extreme precipitation events are predicted to occur more frequently and will have significant influences on terrestrial ecosystem carbon (C) cycling in the future. However, response patterns of soil respiration to precipitation changes remain uncertain in terrestrial ecosystems. A field experiment with seven precipitation treatments (i.e. from −60% to +60% of ambient precipitation to form a drought to wet precipitation gradient) was conducted over three growing seasons (2010–2012) in a semiarid temperate steppe of Northern China. Results showed a nonlinear response pattern of soil respiration along the experimental precipitation gradient, with soil respiration suppressed by decreased precipitation and enhanced by increased …

Draft Genome Sequences Of Three Cellulolytic Bacillus Licheniformis Strains Isolated From Imperial Geyser, Amphitheater Springs, And Whiterock Springs Inside Yellowstone National Park, Joshua A. O'Hair, Hui Li, Santosh Thapa, Matthew Scholz, Suping Zhou

Agricultural and Environmental Sciences Faculty Research

Novel cellulolytic microorganisms are becoming more important for rapidly growing biofuel industries. This paper reports the draft genome sequences of Bacillus licheniformis strains YNP2-TSU, YNP3-TSU, and YNP5-TSU. These cellulolytic isolates were collected from several hydrothermal features inside Yellowstone National Park.

Hairy Root Transgene Expression Analysis Of A Secretory Peroxidase (Pvpox1) From Common Bean Infected By Fusarium Wilt, Renfeng Xue, Xingbo Wu, Yingjie Wang, Yan Zhuang, Jian Chen, Jing Wu, Weide Ge, Lanfen Wang, Shumin Wang, Matthew W. Blair

Agricultural and Environmental Sciences Faculty Research

Plant peroxidases (POXs) are one of the most important redox enzymes in the defense responses. However, the large number of different plant POX genes makes it necessary to carefully confirm the function of each paralogous POX gene in specific tissues and disease interactions. Fusarium wilt is a devastating disease of common bean caused by Fusarium oxysporum f. sp. phaseoli. In this study, we evaluated a peroxidase gene, PvPOX1, from a resistant common bean genotype, CAAS260205 and provided direct evidence for PvPOX1’s role in resistance by transforming the resistant allele into a susceptible common bean genotype, BRB130, via hairy root transformation …