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Articles 1 - 30 of 1030
Full-Text Articles in Life Sciences
Utilizing Soil Sensors To Assess Soil Health And Investigating Cover Crops Impact On Methane Emission, Shailesh Pandit
Utilizing Soil Sensors To Assess Soil Health And Investigating Cover Crops Impact On Methane Emission, Shailesh Pandit
Electronic Theses and Dissertations
Soil health is influenced by climate change, cultural practices, topography, crop rotations, cover crops, soil parent materials, and soil biota. Interactions between these factors can improve or reduce soil health. This thesis investigates two topics, using soil sensors to provide a rapid assessment of soil health, and the impact of cover crops on soil biological activity and greenhouse gas emissions. Chapter 1 explores the use of two types of sensors to measure apparent electrical conductivity (ECa) and provides examples on the use of these sensors. Even though both sensors measure ECa using different processes, they provide useful information about temporal …
Valuation Of Soybean Meal Versus Distillers Co-Products As A Protein Supplement For Finishing Beef Cattle: Effects On Growth Performance, Carcass Characteristics, And Efficiency Of Dietary Net Energy Utilization, Cassidy R. Ross
Electronic Theses and Dissertations
Two randomized complete block design experiments were conducted to: 1) examine the effects of soybean meal with or without additional soybean hulls in replacement of modified corn distillers grains plus (MDGS) solubles on growth performance efficiency of dietary net energy utilization and carcass traits responses in finishing beef steers, 2) determine if partial or complete replacement of dried distillers grains plus solubles (DDGS) with solvent-extracted soybean meal (SBM) in diets based upon high-moisture ensiled corn influences growth performance, efficiency of dietary net energy utilization, sera urea-N (SUN) concentrations, or carcass traits in finishing beef steers. Experiment 1 was a 118-d …
Evaluation Of Dietary Fiber Technologies In Sow Diets, Garrin Shipman
Evaluation Of Dietary Fiber Technologies In Sow Diets, Garrin Shipman
Electronic Theses and Dissertations
Various technologies and alternative ingredients are being developed to increase the nutrient value of fibrous feedstuffs, improve animal performance, and promote sustainable swine production. Fiber-degrading enzymes have been extensively studied in growing pigs, with few studies focused on gestating sows. When considering gestating sows have greater energy digestibility of fibrous feedstuffs than grower pigs, the efficacy of fiber-degrading enzymes needs to be determined for implementation in commercial sow diets. As fiber inclusion in gestation diets benefits sow performance, corn dried distiller grains with solubles (DDGS) are a candidate fiber source for gestation alternative energy ingredient. Functional fibers, such as yeast …
Nitrogen Application Timing And Cereal Rye (Secale Cereale L.) Cover Crop Influence Greenhouse Gas Emissions, Graig Reicks
Nitrogen Application Timing And Cereal Rye (Secale Cereale L.) Cover Crop Influence Greenhouse Gas Emissions, Graig Reicks
Electronic Theses and Dissertations
Dryland contributions to greenhouse gas (GHG) emissions are increased by N fertilizer applications and high soil water contents. Fertilizer timing and spring growth of cover crops prior to cash crop planting were investigated in separate studies to examine impacts on overall GHG emissions using a near continuous measurement system. There was a significant interaction between N fertilizer rate (0 vs. 224 kg N ha-1 surface-applied as urea) and application date (p= 0.01) for CO2 emissions. This interaction occurred because N fertilizer increased CO2 emissions by 35% for the 21 d interval following early spring application. When application was delayed until …
Influence Of Reduced Salt In Fully Cooked Ham And Sausage Cured With Natural Celery Powder On The Growth Of Listeria Monocytogenes At 4⁰C, Anne Sherod
Electronic Theses and Dissertations
This research evaluated the influence of reducing salt on outgrowth of L. monocytogenes on ready to eat (RTE) deli-style sliced ham cured with natural celery powder, fully cooked sausage links cured with traditional sodium nitrite and sausage links cured with natural celery powder, vacuum packaged and stored at 4⁰C on the outgrowth of L. monocytogenes. Experiment 1 evaluated L. monocytogenes outgrowth on ham over 153 days on the following treatments, current retail ham (HC) 2.0% salt; reduced salt 1 (HRS1) 1.5% salt; reduced salt 2 (HSR2) 1.35% salt; and reduced salt 2 without antimicrobials (HRS2WA) 1.35% salt without potassium lactate …
Development Of Innovative Flocculation Technologies For Agricultural Water Treatment, Noor Haleem
Development Of Innovative Flocculation Technologies For Agricultural Water Treatment, Noor Haleem
Electronic Theses and Dissertations
The development of innovative flocculation technologies is essential for addressing the challenges of agricultural water treatment. These technologies play a crucial role in removing contaminants such as suspended solids and nutrients, thereby ensuring safer water for irrigation and livestock consumption. By enhancing water quality and wastewater management, they contribute significantly to environmental sustainability and public health in agricultural communities. This comprehensive thesis extensively explores various dimensions of flocculation, with a focused effort on methodologies and resources aimed at strengthening sustainability and efficiency. A pivotal aspect of the research involves synthesizing cationic starch (CS), a flocculant derived from an underutilized resource, …
Advancing Biological Applications Through Microfluidic-Based Tool Development, K.M. Taufiqur Rahman
Advancing Biological Applications Through Microfluidic-Based Tool Development, K.M. Taufiqur Rahman
Electronic Theses and Dissertations
This research undertook an interdisciplinary approach, integrating bioengineering, microbiology, molecular biology, and systems biology to investigate bacterial dynamics behavior. Specifically, it delved into the development of microfluidic devices for biological applications such as bacterial cell counts, real-time observation of plant roots (here, specialized lectin-coated microbeads are used that mimic root characteristics), and soil microbe interactions. Furthermore, Next-Generation Sequencing and systems biology methodologies were employed to explore the intricate, multifaceted survival mechanisms of Escherichia coli persister population. Studying and quantifying persisters or testing for the existence of VBNC (viable but nonculturable) is challenging. These experiments require precise counts. It has been …
Effects Of Storage Regimes On Chemistry, Functional Properties, And Vitamin Profile Of Different Varieties Of Chickpea, Shirin Kazemzadeh Pournaki
Effects Of Storage Regimes On Chemistry, Functional Properties, And Vitamin Profile Of Different Varieties Of Chickpea, Shirin Kazemzadeh Pournaki
Electronic Theses and Dissertations
Chickpea (cicer arietinum) samples from five distinct varieties (crown, royal, orion, sierra, and frontier) were subjected to varying storage conditions to investigate the impact of temperature and relative humidity (rh) on their chemical, functional, physical properties, and vitamin b profile. over 360 days, samples were stored at two temperatures (21°c and 40°c) and three rh levels (40%, 55%, 65%), with collections made every 90 days. initial observations focused on key chemical properties including moisture, protein, fat, and total starch content. subsequently, protein and starch fractions were isolated for further analysis, employing techniques such as sodium dodecyl sulfate-polyacrylamide gel electrophoresis (sds-page) …
Developing Machine Learning Models For Selection Of Management Zones, Sravanthi Bachina
Developing Machine Learning Models For Selection Of Management Zones, Sravanthi Bachina
Electronic Theses and Dissertations
Soil sampling and analyses play a crucial role in optimizing nutrient management and enhancing crop productivity. However, collecting representative samples across diverse landscapes is challenging due to knowledge gaps about spatial variability of soil properties, large fields, multiple samples, and analysis costs. Collecting soil samples based on the management zones can help farmers gather precise information about soil properties with fewer samples. Recent developments in precision agriculture and machine learning. This study aimed to develop machine learning models that can learn, analyze, and refine landscape and soil properties data for automated selection of soil sampling zones and generating prediction maps. …
Physicochemical And Functional Properties Of Different Varieties Of Spring And Winter Peas Of South Dakota, Prakriti Dhakal
Physicochemical And Functional Properties Of Different Varieties Of Spring And Winter Peas Of South Dakota, Prakriti Dhakal
Electronic Theses and Dissertations
Peas are a good source of nutrients for both humans and animals, and they are the world's second most significant and sustainable legume. There have been studies on different kinds of pea grown in other parts of the world, but there is no information on the compositional and functional characteristics of pea varieties grown specifically in South Dakota. The objective of the study was to evaluate the proximate composition and functional properties of various pea varieties and determine if the pea variety impacted cookie properties. The proximate composition (moisture, protein, starch, and amylose), functional capacities (water holding, oil holding capacity, …
Portable X-Ray Fluorescence Spectrometry For Sensing Salinity And Sodicity In Glacial Northern Great Plains Soils With Machine Learning Models, Adam Devlin
Electronic Theses and Dissertations
Saline and sodic soils are an increasing concern across the Northern Great Plains (NGP) due to factors of climate change and land management that are drawing geologically derived salts to the land surface. Traditional laboratory assessments, such as electrical conductivity (EC) and sodium adsorption ratio (SAR), are time and resource consumptive. Portable X-ray fluorescence (PXRF) may be a viable proximal sensing alternative, as it is able to provide elemental data in minutes, in situ or ex situ, and can directly quantify salinity-associated elements like Ca, Mg, and S. PXRF paired with predictive models has proven useful for a range of …
Integrating Early Season Clover Cover Crops As A Living Mulch In Broccoli And Transitional Organic Winter Squash Production In Eastern South Dakota, Alexis Rose Barnes
Integrating Early Season Clover Cover Crops As A Living Mulch In Broccoli And Transitional Organic Winter Squash Production In Eastern South Dakota, Alexis Rose Barnes
Electronic Theses and Dissertations
Managing weeds, improving soil health, and reducing the use of plastic mulch continue to be priorities for South Dakota vegetable farmers. Farmers have expressed an interest in integrating cover crops into their farm systems. Clover cover crops used as a living mulch within and along cash crop rows may aid in weed suppression, nitrogen fixation, and prevent soil erosion. However, prior research has shown challenges of incorporating living mulch due to yield decreases. Research conducted in 2022 and 2023 in eastern South Dakota investigated the effects of four clover and four in-row soil management treatments on broccoli and organic winter …
Effects Of Low-Cost, Low-Tech Tools For Riparian Restoration On Prairie Streams In Western South Dakota, James Andrew Joseph Bolyard
Effects Of Low-Cost, Low-Tech Tools For Riparian Restoration On Prairie Streams In Western South Dakota, James Andrew Joseph Bolyard
Electronic Theses and Dissertations
Despite making up less than two percent of the overall landscape in the arid and semi-arid western US, riparian areas are a crucial resource for agriculture, livestock, and wildlife. However, many have impaired function and reduced riparian cover. Low-Cost, Low-Tech Tools (LCLTT) are a subset of Process-Based Restoration (PBR) used for riparian restoration that were chosen for their cost-effectiveness and minimal technical requirements. LCLTT has been tested in mountainous areas of the western US but only recently implemented in the Northern Great Plains (NGP). Given their novelty as an approach toward restoration for the region, professional restoration and landowner communities …
The Hutton Project: Long-Term Agricultural Impacts On Soil Loss And Carbon Dynamics In Eastern South Dakota, Eli Halverson
The Hutton Project: Long-Term Agricultural Impacts On Soil Loss And Carbon Dynamics In Eastern South Dakota, Eli Halverson
Electronic Theses and Dissertations
Long-term and intensified agricultural land management has resulted in increased rates of soil erosion and has altered much of the carbon cycle at regional and global scales. Anthropogenic degradation of soil resources is a barrier to sustainable production, soil functioning, and ecosystem services. It is difficult to quantify the scope of pedogenic changes due to the lack of legacy data and short temporal scales. This study utilized decades to century-old soil information to quantify historical soil erosion losses and changes in soil carbon pools of eastern South Dakota soils. The results show that soils in the region have been significantly …
The Spatial Ecology Of Plains Spotted Skunks In South Dakota: Insights From Species Distribution, Resource Selection, And Co-Occurrence Models, Kara M. White
Electronic Theses and Dissertations
The plains spotted skunk (Spilogale interrupta) is a data-deficient small carnivore native to the central plains of North America that has experienced significant population declines. My dissertation investigates the species’ distribution, habitat associations, and interspecific interactions in South Dakota, providing crucial information for conservation actions. In Chapter 1, I developed ensemble distribution models on a broad scale, incorporating species location data from 1985–2020, environmental factors, and six predictive modeling algorithms. I identified key predictors including mean temperature diurnal range and proportion of area as pasture, and estimated ~31,300 km2 of potential habitat, predominately in eastern South Dakota. In Chapter 2, …
Species Distribution Modeling Of Aquilegia Brevistyla (Ranunculaceae): A Critically Imperiled Black Hills Disjunct Species, Eric Daniel Puetz
Species Distribution Modeling Of Aquilegia Brevistyla (Ranunculaceae): A Critically Imperiled Black Hills Disjunct Species, Eric Daniel Puetz
Electronic Theses and Dissertations
Unchecked human activity is contributing to rising levels of greenhouse gas emissions, changes in land use, altered disturbance/fire regimes, spread of invasive species, and loss of biological diversity and related breakdown of ecosystem services. Additionally, climatic shifts may lead to phenological mismatches between species and their environments if these changes outpace a species’ ability to adapt or migrate to a more suitable habitat. Isolated mountain populations are particularly threatened by unpredictable climatic conditions, as they may have limited migration corridors and often lower levels of genetic diversity to move or adapt, respectively. As these negative feedbacks compound on the landscape, …
Longevity In Afrikaner Cattle: A Survival Analysis, Allison M. Bot Steffl
Longevity In Afrikaner Cattle: A Survival Analysis, Allison M. Bot Steffl
Electronic Theses and Dissertations
The Afrikaner breed of cattle is indigenous to South Africa and due to their hardiness was once the most popular breed amongst farmers. The objectives for this study were to: (1) estimate environmental effects (age at first calving, season) and (2) genetic variance affecting longevity in Afrikaner cattle using survival analysis and a Weibull model and (3) evaluate the effect of the Infusion Project on longevity utilizing a Cox model. For the first objective, age at first calving impacted longevity as younger age at first calving was associated with decreased longevity. Season of birth and year of birth also impacted …
Nutrient Intake, Performance, And Body Composition In Preseason Wrestlers, Gregory Coapstick
Nutrient Intake, Performance, And Body Composition In Preseason Wrestlers, Gregory Coapstick
Electronic Theses and Dissertations
BACKGROUND: College athletes, especially in weight class sports, often experience energy deficits. Weight class sports such as wrestling are at greatest risk for deficiencies, and little is known about the relationships between body composition, nutrient intake, and performance in these athletes.
OBJECTIVES: The purposes of this study were to quantify macronutrient and micronutrient intake of pre-season male collegiate wrestlers and compare to recommendations, and examine relationships among nutritional intakes, body composition, and performance measurements of strength, anaerobic, and aerobic capacity.
METHODS: Male Division I wrestlers (n=11, mean ± SD age: 21.3±1.7 years, wrestling experience: 14.9±2.5 years) were recruited during preseason. …
Estimating South Dakota’S Capacity To Meet K-12 School Meal Fruit And Vegetable Requirements With State-Grown Produce, Bridgette Bienias
Estimating South Dakota’S Capacity To Meet K-12 School Meal Fruit And Vegetable Requirements With State-Grown Produce, Bridgette Bienias
Electronic Theses and Dissertations
Objective: Quantify South Dakota (SD)’s capacity to procure locally-sourced fruit and vegetables (FVs) for K-12 school meals.
Methods: Secondary analysis of 2017 SD agricultural census and school meal data. Yields were converted to edible cups of FVs using school meal guidelines provided by the U.S. Department of Agriculture. Student enrollment and meal redemptions were used to estimate the number of FV cups needed.
Results: Estimated SD FV production met minimum total K-12 cup requirements, except for total fruit (66%) and dark green vegetables (80%). Legumes supplied 77% of the total cups of vegetables produced in 2017, whereas dark green vegetables …
The Relationship Of Macronutrient Intake With Growth In Children With Type 1 Diabetes Mellitus, Jessica L. Freeling
The Relationship Of Macronutrient Intake With Growth In Children With Type 1 Diabetes Mellitus, Jessica L. Freeling
Electronic Theses and Dissertations
Child growth, a sensitive metric of overall health, results from the intricate interplay of nature and nurture. While the importance of nutrition in child growth is well established, growth trajectories exhibit substantial individual variability, influenced by sex and age, and often characterized by nonlinear patterns. Inadequate nutrition or disease can hinder growth, with potential for recovery upon proper nutrition or acute disease resolution. However, chronic disease or persistent malnutrition may lead to permanent growth perturbation. Notably, before the advent of insulin, chronic stunting and wasting were hallmarks of Type 1 diabetes mellitus (T1DM), though the specific impacts under modern standard …
Evaluation Of Yeast Postbiotic In Sow Diets On Sow And Offspring Performance And Microbial Succession, Joel Kieser
Evaluation Of Yeast Postbiotic In Sow Diets On Sow And Offspring Performance And Microbial Succession, Joel Kieser
Electronic Theses and Dissertations
Litter size and the resulting nutritional demand on the sow continue to increase while sow mortality and culling rate are also increasing. Non-nutritive feed additives may enhance sow health and thereby improve offspring growth and productivity after weaning. Development of the gut microbiome in piglets via microbial succession is critical for maximizing their productivity and providing stability for overcoming weaning stress. The objective of this study was to evaluate the impact of yeast-based postbiotic supplementation in gestation and lactation diets on offspring performance through the nursery period and on whether a yeast postbiotic could impact the sow fecal microbiome as …
In-Vivo Assessment Of A Direct-Fed Microbial On Lactation Performances, Blood Biomarkers, Ruminal Fermentation And Microbial Abundance In Transition To Mid-Lactation Holstein Cows, Marcela Bulnes Lopez
In-Vivo Assessment Of A Direct-Fed Microbial On Lactation Performances, Blood Biomarkers, Ruminal Fermentation And Microbial Abundance In Transition To Mid-Lactation Holstein Cows, Marcela Bulnes Lopez
Electronic Theses and Dissertations
The transition period is a crucial stage in the lactation cycle and can lead to significant metabolic changes in cows. The use of nutritional interventions, such as direct-fed microbials, may assist cows during the transition from pregnancy to early lactation, reducing the occurrence of metabolic disorders and improving overall health. The primary goal of this study was to assess the effects of a commercial rumen-derived directfed microbial (DFM) product (Galaxis™ Frontier (GF) Native Microbials Inc., CA, USA) on various factors such as performance, blood biomarkers, rumen fermentation, and bacterial population in dairy cows during the transition period up to 100 …
Fungicide Sensitivity In P. Tritici-Repentis Diverse Population And Phenotyping Of Spelt Wheat For Multiple Diseases, Zunera Shabbir
Fungicide Sensitivity In P. Tritici-Repentis Diverse Population And Phenotyping Of Spelt Wheat For Multiple Diseases, Zunera Shabbir
Electronic Theses and Dissertations
Pyraclostrobin, a QoI fungicide, is extensively used in the management of fungal leaf spot diseases. In this study, 215 P. tritici-repentis isolates collected from wheat fields in South Dakota were evaluated for their sensitivity to Pyraclostrobin. Of 215 isolates, 48 isolates (22%) exhibited insensitivity based on G143A mutation and EC50 values using spore germination assay. Our results indicate reduced sensitivity to insensitivity to pyraclostrobin in some isolates suggesting regular monitoring of the P. tritici-repentis population to QoI fungicides is essential to track the evolution of insensitive population. Spelt wheat (Triticum spelta L.) is an ancient cereal that is gaining small …
Indigenous Toxin Affects Cell Viability, While An Artificial Proteolytic Queueing Causes The Upregulation Of Specific Genes, Mohammed Salahuddin
Indigenous Toxin Affects Cell Viability, While An Artificial Proteolytic Queueing Causes The Upregulation Of Specific Genes, Mohammed Salahuddin
Electronic Theses and Dissertations
Studying bacterial physiology is crucial to understand the fundamental mechanisms that govern bacterial growth, survival, and adaptation. This thesis combines two chapters investigating bacterial physiology by studying important cellular processes like bacterial toxin-antitoxin systems and proteolytic pathways. The first chapter involves studying bacterial toxin-antitoxin systems to understand the regulation and function of hypothetical toxin-antitoxin (TA) systems of bacteria and the effects of these TA systems on bacterial growth and survival. The emergence of these genetic modules in bacterial research and the unrevealing of some of their important roles in cell physiology in recent years has drawn much attention in scientific …
Regional Assessment Of Live Cattle And Carcass Characteristics In The Northern Plains, Harlee A. Brauckmuller
Regional Assessment Of Live Cattle And Carcass Characteristics In The Northern Plains, Harlee A. Brauckmuller
Electronic Theses and Dissertations
The goal of this project was to perform a regional assessment of live cattle and carcass characteristics in the northern plains. The assessment audited three packing plants to assess fed cattle, cow, and bull production. The live animal factors evaluated were transportation, mobility of cattle, identification, brands, and mud/manure present. Data collected during the slaughter process was bruising, dentition, and offal condemnations. In fed cattle yield grade, quality grade, and hot carcass weight were assessed. In cows and bulls muscle score and fat color score were assessed. To assess differences between cattle in the Northern Plains and cattle in the …
Characterizing Vaginal Gene Expression In Prepubertal Gilts, Shannon Lee Dierking
Characterizing Vaginal Gene Expression In Prepubertal Gilts, Shannon Lee Dierking
Electronic Theses and Dissertations
The best-known predictor of reproductive success in gilts is the age at puberty. Early puberty (i.e., d170 – d180 of age) is associated with improved long-term reproductive performance and more full value offspring for market. The ability to predict, in the prepubertal stage, the age that a replacement gilt would be expected to achieve puberty has great reproductive and economic advantages. This work focuses on identifying biological markers that are potentially predictive of age at puberty. Study 1 identified changes in the vaginal epithelium during gilt reproductive development. Pre-pubertal gilts (n =13) were followed from d70 of age until first …
Unveiling Race Diversity And Fungicide Sensitivity Profiles Of Pyrenophora Tritici Repentis Populations In South Dakota Collected From 2021-2023, Jaswinder Kaur
Unveiling Race Diversity And Fungicide Sensitivity Profiles Of Pyrenophora Tritici Repentis Populations In South Dakota Collected From 2021-2023, Jaswinder Kaur
Electronic Theses and Dissertations
Tan spot, a foliar disease of wheat caused by the ascomycetes fungus Pyrenophora tritici repentis (Ptr), poses a significant threat to wheat crops globally. The disease gained economic importance in the late 1970s due to shifts in farming practices, such as minimal tillage and planting on crop residues, providing favorable conditions for the pathogen to overwinter and cause infections. Ptr is classified into eight races based on the production of necrotrophic effectors—Ptr ToxA (necrosis-inducing), Ptr ToxB, and Ptr ToxC (chlorosis-inducing on respective susceptible and sensitive cultivars)—resulting in distinct symptoms on susceptible wheat differentials. The inverse gene-for-gene hypothesis of Ptr suggests …
Evaluation Of Feed Additives And Delivery Method For Receiving And Finishing Cattle, Thiago Lauro Maia Ribeiro
Evaluation Of Feed Additives And Delivery Method For Receiving And Finishing Cattle, Thiago Lauro Maia Ribeiro
Electronic Theses and Dissertations
Trace minerals (TM) and vitamins are essential to improve production output and efficiency in beef cattle. The first experiment was conducted to investigate if the delivery method of TM and Saccharomyces cerevisiae yeast culture influence growth performance and hepatic TM content of receiving steers. The second experiment evaluated the effect of supplemental protected B - vitamins to finishing steers. Experiment 1 used Charolais x Angus steers calves (n = 192; 256 ± 14 kg) in a 49-d receiving experiment. Within 36h of weaning, steers were weighed, allotted to 24 pens (n = 8 steers/pen; 8 pens/treatment) and randomly assigned to …
Extraction, Characterization, And Utilization Of Yellow Pea Starch, Abdulmalik Albu Tuwaybah
Extraction, Characterization, And Utilization Of Yellow Pea Starch, Abdulmalik Albu Tuwaybah
Electronic Theses and Dissertations
Dry peas, a leguminous crop, are one of the world's oldest crops, accounting for 35–40% of total pulse trade. Pea starch is an inexpensive source of starch since it can be obtained as a by-product of protein extraction. Recovering the starch from protein isolation streams (i.e., before or after) improves the environmental impact of the protein isolation process. The goal of the study was to transform low-value pea starch into highvalue starch through starch extraction and food application. Two methods of starch extraction were used (47.5% ethanol treatment and SC CO2 + EtOH extraction). Starch samples were analyzed for chemical, …
Seroprevalence Of Sars-Cov-2 Antibodies Among Rural Healthcare Workers, Jordan Neises
Seroprevalence Of Sars-Cov-2 Antibodies Among Rural Healthcare Workers, Jordan Neises
Electronic Theses and Dissertations
The objective of this longitudinal cohort study was to determine the seroprevalence of antibodies to severe acute respiratory syndrome coronavirus-2 (SARSCoV- 2) in healthcare workers employed at healthcare clinics in three rural counties in eastern South Dakota and western Minnesota from May 13, 2020 through December 22, 2020. Three blood draws were performed at five clinical sites and tested for the presence of antibodies against the SARS-CoV-2 virus. Serum samples were tested for the presence of antibodies using a fluorescent microsphere immunoassay (FMIA), neutralization of SARS-CoV-2 Spike-pseudotyped particles (SARS-CoV-2pp) assay, and serum virus neutralization (SVN) assay. The seroprevalence was determined …