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Kryptoperidinium Foliaceum Blooms In South Carolina: A Multi–Analytical Approach To Identification, Torstein Tengs
Kryptoperidinium Foliaceum Blooms In South Carolina: A Multi–Analytical Approach To Identification, Torstein Tengs
Dr. Torstein Tengs
Observations following the discovery of Kryptoperidinium foliaceum blooms in South Carolina (SC), USA, suggest that a multi-analytical approach, using a standard, minimal set of criteria, should be adopted for determining dinoflagellate species identity and taxonomic placement. A combination of morphological, molecular, and biochemical analyses were used to determine the identity of this “red tide” dinoflagellate, first documented in SC waters in the spring of 1998. Results from thecal plate tabulations (based on scanning electron and epifluorescence microscopy), gene sequence data, species-specific PCR probe assays, and microalgal pigment profiles were analyzed and compared to reference cultures of K. foliaceum. Comparative data …
No Difference Found In Ribosomal Dna Sequences From Physiologically Diverse Clones Of Karenia Brevis (Dinophyceae) From The Gulf Of Mexico, Torstein Tengs
No Difference Found In Ribosomal Dna Sequences From Physiologically Diverse Clones Of Karenia Brevis (Dinophyceae) From The Gulf Of Mexico, Torstein Tengs
Dr. Torstein Tengs
Maximum growth rate and toxin content were significantly different among five strains of Karenia brevis isolated from Texas and Florida when grown under identical culture conditions. Sequence analysis of the 18S rRNA gene and internal transcribed spacer (ITS) regions revealed, however, that all five strains were identical. Consequently, a clear genetic basis for physiological variability among various geographical isolates of K. brevis from the Gulf of Mexico could not be assessed using these genetic markers. Both the ITS and 18S rRNA regions may be useful in species-specific probe selection. At the intra-specific level, however, an alternative marker will be needed …
Discovery Of The Toxic Dinoflagellate Pfiesteria In Northern European Waters, Torstein Tengs
Discovery Of The Toxic Dinoflagellate Pfiesteria In Northern European Waters, Torstein Tengs
Dr. Torstein Tengs
Several dinoflagellate strains of the genus Pfiesteria were isolated by culturing techniques from sediment samples taken in the Oslofjord region of Norway. Pfiesteria piscicida, well known as a fish killer from the Atlantic coast of America, was identified by genetic methods and light microscopy. The related species Pfiesteria shumwayae was attracted from the sediment by the presence of fish, and has proved toxic. This present survey demonstrates the wide distribution of these potentially harmful species, but so far they have not been connected with fish kills in Europe.