Open Access. Powered by Scholars. Published by Universities.®
Articles 1 - 2 of 2
Full-Text Articles in Life Sciences
Transcript Abundance Responses Of Resistance Pathways Of Arabidopsis Thaliana To Deoxynivalenol, David A. Lightfoot
Transcript Abundance Responses Of Resistance Pathways Of Arabidopsis Thaliana To Deoxynivalenol, David A. Lightfoot
David A. Lightfoot
Mycotoxin deoxynivalenol (DON), produced by Gibberella zeae (Schwein.) Petch (teleomorph of Fusarium graminearum Schwabe) was known to be both a virulence factor in the pathogenesis of Triticum aestivum L. (wheat) and an inhibitor of Arabidopsis thaliana L. seed germination. Fusarium graminearum causes both Gibberella ear rot in maize (Zea mays L.) and Fusarium head blight (FHB) in wheat and barley. Arabidopsis thaliana was also a host for the related root rot pathogen F. virguliforme Aoki. A. thaliana seedling growth was reduced by the pathogen in a proportional response to increasing spore concentrations. Here, the changes in transcript abundances corresponding to …
Homo-Dimerization And Ligand Binding By The Leucine-Rich Repeat Domain At Rhg1/Rfs2 Underlying Resistance To Two Soybean Pathogens, David A. Lightfoot
Homo-Dimerization And Ligand Binding By The Leucine-Rich Repeat Domain At Rhg1/Rfs2 Underlying Resistance To Two Soybean Pathogens, David A. Lightfoot
David A. Lightfoot
Background: The protein encoded by GmRLK18-1 (Glyma_18_02680 on chromosome 18) was a receptor like kinase (RLK) encoded within the soybean (Glycine max L. Merr.) Rhg1/Rfs2 locus. The locus underlies resistance to the soybean cyst nematode (SCN) Heterodera glycines (I.) and causal agent of sudden death syndrome (SDS) Fusarium virguliforme (Aoki). Previously the leucine rich repeat (LRR) domain was expressed in Escherichia coli. Results: The aims here were to evaluate the LRRs ability to; homo-dimerize; bind larger proteins; and bind to small peptides. Western analysis suggested homo-dimers could form after protein extraction from roots. The purified LRR domain, from residue 131–485, …