Maldi-Tof Ms As A Supportive Tool For The Evaluation Of Bacterial Diversity In Soils From Africa And The Americas, Adrian Douglas Allen, Maria Velez-Quinones, Broderick Eribo, Vernon Morris
Sep 2014
Maldi-Tof Ms As A Supportive Tool For The Evaluation Of Bacterial Diversity In Soils From Africa And The Americas, Adrian Douglas Allen, Maria Velez-Quinones, Broderick Eribo, Vernon Morris
Broderick Eribo
Identification and characterization of viable-culturable bacteria (VCB) associated with soils from Africa and the Americas is significant for environmental and battlefield security. Such analyses are scarce and their evaluation using traditional microbiological methods do not fully elucidate the structure and chemotaxonomic characteristics of the microbial community. In this study, matrix assisted laser desorption time of flight mass spectrometry (MALDI-TOF MS) spectrometry in addition to 16S rRNA sequencing, and diversity indices were employed to characterize VCB and their associated biomarkers. Nineteen genera were identified across all sample locations, but only four (Bacillus, Brevibacillus, Paenibacillus and Terribacillus) confirmed by ClustalW2 as being …
Leptospire Genomic Diversity Revealed By Microarray-Based Comparative Genomic Hybridization, Broderick Eribo, Sirima Mingmongkolchai, Tingfen Yan, Padunsri Dubbs, Karen E. Nelson
Jan 2012
Leptospire Genomic Diversity Revealed By Microarray-Based Comparative Genomic Hybridization, Broderick Eribo, Sirima Mingmongkolchai, Tingfen Yan, Padunsri Dubbs, Karen E. Nelson
Broderick Eribo
Comparative genomic hybridization was used to compare genetic diversity of five strains of Leptospira (Leptospira interrogans serovars Bratislava, Canicola, and Hebdomadis and Leptospira kirschneri serovars Cynopteri and Grippotyphosa). The array was designed based on two available sequenced Leptospira reference genomes, those of L. interrogans serovar Copenhageni and L. interrogans serovar Lai. A comparison of genetic contents showed that L. interrogans serovar Bratislava was closest to the reference genomes while L. kirschneri serovar Grippotyphosa had the least similarity to the reference genomes. Cluster analysis indicated that L. interrogans serovars Bratislava and Hebdomadis clustered together first, followed by L. interrogans serovar Canicola, …
Influence Of Iron-Chelated Growth Conditions On Outer Membrane Protein Production And Virulence Of Vibrio Tubiashii, Broderick Eribo
Aug 2011
Influence Of Iron-Chelated Growth Conditions On Outer Membrane Protein Production And Virulence Of Vibrio Tubiashii, Broderick Eribo
Broderick Eribo
Growth of two Vibrio tubiashii strains under iron-chelated conditions resulted in the production of a hydroxymate-like siderophore, and expression of outer membrane proteins with homologies to proteins in Vibrio cholerae and Vibrio vulnificus which were not seen in cells grown under non-chelated growth conditions. PCR analysis using primers based on Listonella anguillarum's ferric uptake Repressor protein (fur) gene detected a 316 bp fur gene homolog which also had sequence homology to the fur genes of V. cholerae and V. vulnificus. V. tubiashii cultured under iron-chelated growth conditions induced a greater fluid accumulation (FA) response in suckling mice than cells which …
Biological Activity And Mass Spectrometric Analysis Of Vernonia Amygdalina Fractions, Broderick Eribo
Dec 2010
Biological Activity And Mass Spectrometric Analysis Of Vernonia Amygdalina Fractions, Broderick Eribo
Broderick Eribo
Vernonia amygdalina (VA) is a tropical African plant of the Asteraceae family used as dietary supplement and as a medicinal herb. In this study, fatty acid esters, fatty acid amide, triterpene, diterpene alcohols and phytostanols were identified as the major chemical groups in the aqueous and organic fractions of VA leaf extracts. Their structures were elucidated, on the basis of GC-MS data, as hexanedioic acid, bis (2-ethylhexyl) ester (2a), erucamide (2b) and squalene (2c) in the water fraction; hexadecanoic acid methyl ester (3a), 9(Z)12(Z)15(Z)-octadecatrienoic acid, methyl ester (3b) and phytol (3c) in methanol fraction; phytol (4a), squalene (4b) and 7,22-ergostadienol …
Occurrence Of Pediocin Pa-1/Ach-Like Bacteriocin In Native Non-Starter Lactobacillus Casei, Lactobacillus Paracasei And Lactobacillus Rhamnosus From Retail Cheddar Cheese, Mothomang Mlalazi, Angela Winslow, Junia Jean-Gilles Beaubrun, Broderick Eribo
Dec 2010
Occurrence Of Pediocin Pa-1/Ach-Like Bacteriocin In Native Non-Starter Lactobacillus Casei, Lactobacillus Paracasei And Lactobacillus Rhamnosus From Retail Cheddar Cheese, Mothomang Mlalazi, Angela Winslow, Junia Jean-Gilles Beaubrun, Broderick Eribo
Broderick Eribo
Bacteriocin producing Lactobacillus paracasei, Lactobacillus casei and Lactobacillus rhamnosus were isolated from retail Cheddar cheese and identified by both phenotypic and molecular methods. These isolates were found to produce bacteriocins with broad-spectrum activity against several foodborne pathogens: Listeria monocytogenes, Bacillus cereus, Salmonella typhi, E. coli O157:H7, Enterococcus faecalis, Enterococcus faecium and Pseudomonas aeruginosa. Further characterization of the bacteriocins showed that they had extremely high thermostability as activity was maintained after boiling for 6.5 h. In addition, autoclaving for 45 minutes had no significant effect on the activity. Similarly, storage at -20 ⁰C, 4 ⁰C, 25 ⁰C, 37 ⁰C and 45 …
Isolation And Characterization Of Vibrio Tubiashii Outer Membrane Proteins And Determination Of A Toxr Homolog, Broderick Eribo
Jan 2008
Isolation And Characterization Of Vibrio Tubiashii Outer Membrane Proteins And Determination Of A Toxr Homolog, Broderick Eribo
Broderick Eribo
Outer membrane proteins (OMPs) expressed by Vibrio tubiashii under different environmental growth conditions were characterized by sodium dodecyl sulfate-polyacrylamide gel electrophoresis, N-terminal amino acid sequencing, and PCR analyses. Results showed the presence of a 38- to 40-kDa OmpU-like protein and ompU gene, a maltoporin-like protein, several novel OMPs, and a regulatory toxR homolog.
Determination Of The Efficacy Of The Antigen Extraction Technique In The Pathfinder-Eia Chlamydia Detection System, George A. Ndeta, Broderick Eribo, James Gregory
Dec 2002
Determination Of The Efficacy Of The Antigen Extraction Technique In The Pathfinder-Eia Chlamydia Detection System, George A. Ndeta, Broderick Eribo, James Gregory
Broderick Eribo
There is need for a fast and reliable method to detect Chlamydia trachomatis. With new technology, a technologist can be processing newly arrived swab samples at the same time that an old batch is being assayed. A technologist should be familiar with the bright apple-green fluorescence of the Chlamydia organism in the MicroTrack technique.
Characterization Of Partially Transesterified Poly(Β-Hydroxyalkanoate)S By Matrix-Assisted Laser Desorption/Ionization Time-Of-Flight Mass Spectrometry, Broderick Eribo
May 2001
Characterization Of Partially Transesterified Poly(Β-Hydroxyalkanoate)S By Matrix-Assisted Laser Desorption/Ionization Time-Of-Flight Mass Spectrometry, Broderick Eribo
Broderick Eribo
Matrix-assisted laser desorption/ionization time-of-flight mass spectrometry (MALDI-TOFMS) was used for the characterization of a partially transesterified poly(beta-hydroxyalkanoate) (PHA), a polymer produced by the bacterial strain Alcaligenes eutrophus with saponified vegetable oils as the sole carbon sources. The transesterification was carried out separately under acidic and basic conditions to obtain PHA oligomers weighing <10 kDa. The intact oligomers were detected in their cationized forms, [M + Na]+ and [M + K]+, by MALDI-TOFMS. A composition analysis, using the MALDI-TOF spectra, indicated that the oligomers obtained via acid catalysis contained a methyl 3-hydroxybutyrate end group, and those obtained by base catalysis had …
Extracellular Enzyme Production By Rhizopus And Mucor Species On Solid Media, D P. Thompson, Broderick Eribo
Dec 1983
Extracellular Enzyme Production By Rhizopus And Mucor Species On Solid Media, D P. Thompson, Broderick Eribo
Broderick Eribo
Solid media were employed to determine the presence and absence of extracellular enzyme production by two genera of fruit-rot fungi, Rhizopus and Mucor. The results of this investigation revealed that phosphatase was released into the cultural medium by all the fungi examined; however, only R. oryzae, R. tritici, M. mucedo, and M. piriformis showed the possibility of being high producers of the enzyme. Protease, urease, ribonuclease, pectate lyase, and polygalacturonase, at varying levels of activity, were detected, in the majority of the fungi, in the cultural medium.
Fungal Metabolite From Members Of The Genus Rhizopus, D P. Thompson, A W. Smalley, Broderick Eribo
Apr 1981
Fungal Metabolite From Members Of The Genus Rhizopus, D P. Thompson, A W. Smalley, Broderick Eribo
Broderick Eribo
A fluorescent metabolite present in seven members of the genus Rhizopus was isolated. This compound appeared green before spray treatment and purple after spray treatment with p-anisaldehyde in visible light. Subsequent purification and structural elucidation of the isolated compound yielded 1-[2,6,10,14-tetramethyl-17-carbomethyl heptadecyl]-1-[2,6,10,14-tetramethyl-17-methanoyl heptadecyl]-benzene.
