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Telomere Length Dynamics In Human Memory T Cells Specific For Viruses Causing Acute Or Latent Infections, Joel M. O'Bryan, Marcia Woda, Mary Dawn T. Co, Anuja Mathew, Alan Rothman
Telomere Length Dynamics In Human Memory T Cells Specific For Viruses Causing Acute Or Latent Infections, Joel M. O'Bryan, Marcia Woda, Mary Dawn T. Co, Anuja Mathew, Alan Rothman
Alan Rothman
BACKGROUND: Declining telomere length (TL) is associated with T cell senescence. While TL in naive and memory T cells declines with increasing age, there is limited data on TL dynamics in virus-specific memory CD4+ T cells in healthy adults. We combined BrdU-labeling of virus-stimulated T cells followed with flow cytometry-fluorescent in situ hybridization for TL determination. We analyzed TL in T cells specific for several virus infections: non-recurring acute (vaccinia virus, VACV), recurring-acute (influenza A virus, IAV), and reactivating viruses (varicella-zoster virus, VZV, and cytomegalovirus, CMV) in 10 healthy subjects. Additionally, five subjects provided multiple blood samples separated by up …