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Full-Text Articles in Life Sciences

A Temporal Analysis Of The Microbiota And Biofouling Development On Artificial Substrates In The Port Everglades Inlet, Florida, Denise Swack Dec 2021

A Temporal Analysis Of The Microbiota And Biofouling Development On Artificial Substrates In The Port Everglades Inlet, Florida, Denise Swack

All HCAS Student Capstones, Theses, and Dissertations

A pilot project was deployed in Port Everglades Inlet, Florida that aimed to evaluate the biofilm composing the microbiome on ecologically engineered artificial substrates used to build Coastal Marine Infrastructure. In April of 2017, an Articulated Concrete Block Mattress comprised of an ecological engineered concrete substrate and a standard smooth surface control substrates were compared. This study will provide a profile on the microbiome community on artificial substrates within Port Everglades Inlet on bio-enhancing concrete-based solutions in our Coastal Marine Infrastructure. To study the microbial community, the 16s rRNA technology was used in Illumina’s high-throughput DNA sequencing. Samples were collected …


Microscopic And Genetic Characterization Of Bacterial Symbionts With Bioluminescent Potential In Pyrosoma Atlanticum, Alexis Berger, Patricia L. Blackwelder, Tamara Frank, Tracey Sutton, Nina Pruzinsky, Natalie Slayden, Jose V. Lopez Feb 2021

Microscopic And Genetic Characterization Of Bacterial Symbionts With Bioluminescent Potential In Pyrosoma Atlanticum, Alexis Berger, Patricia L. Blackwelder, Tamara Frank, Tracey Sutton, Nina Pruzinsky, Natalie Slayden, Jose V. Lopez

Biology Faculty Articles

The pelagic tunicate pyrosome, Pyrosoma atlanticum, is known for its brilliant bioluminescence, but the mechanism causing this bioluminescence has not been fully characterized. This study identifies the bacterial bioluminescent symbionts of P. atlanticum collected in the northern Gulf of Mexico using several methods such as light and electron microscopy, as well as molecular genetics. The bacteria are localized within the pyrosome light organs. Greater than 50% of the bacterial taxa present in the tunicate samples were the bioluminescent symbiotic bacteria Vibrionaceae as determined by utilizing current molecular genetics methodologies. A total of 396K MiSeq16S rRNA reads provided total pyrosome …