Life Sciences Commons^™

Why Do Symbiodinium Migrate? Evolutionary Conflict In A Host-Symbiont Community, Jacey Lynn Strohecker

Graduate Research Theses & Dissertations

Climate change is an increasing problem for species all around the globe, including coral reef communities, which are among the most diverse and productive in the world. The mutualistic symbiosis between corals and photosynthetic dinoflagellates (symbionts) is central to these ecosystems. The rising temperatures are causing corals to lose their symbionts in a process known as bleaching, thus endangering the entire community. In this context, it is imperative to discover mechanisms that might mitigate the effects of stress on corals. Previous work has established that stress increases the formation of reactive oxygen species (ROS), that is, partially reduced forms of …

Iron Deficiency Anemia And Difficulty Concentrating In A Nationally Representative Sample Of School-Aged Children In The United States., Elizabeth Jennifer Voyles

Graduate Research Theses & Dissertations

The worldwide prevalence of iron deficiency anemia (IDA) among school-aged children is estimated to be 40%. Anemia has been associated with varying degrees of impaired cognitive functioning due to iron’s role in maintaining the neurochemical environment of the prefrontal cortex (PFC). However, limited research is available related to the prevalence of IDA and its resulting adverse effects on cognitive functioning in school-aged children in the United States. Data from two National Health and Nutrition Examination Surveys (NHANES) from 2013-2016 were analyzed to assess the relationship between iron deficiency anemia and difficulty concentrating in 1758 children, ages 5-15 years old. Hemoglobin …

The Link Between Antibiotic Resistance Used For Recombinant Dna Selection And Cellular Phenotype In Rat Liver Epithelial Cells, Doaa Alshammari

Graduate Research Theses & Dissertations

Recombinant DNA plasmids are often used to mediate forced gene overexpression or suppression in mammalian cells, to address questions regarding the role of specific proteins in cell behavior and function. To select for cells harboring such recombinant plasmids, lethal antibiotics such as Puromycin, Geneticin (Neomycin) and Zeocin are frequently used, as plasmids contain selective antibiotic resistance genes that biochemically disarm them. Antibiotic resistance, induced by plasmid-borne genes and chronic exposure to lethal antibiotics can likewise potentially change the phenotype of cells, confounding the conclusions drawn from experiments on gene overexpression or suppression. Yet, little is known about the effects of …

Morphometric Analysis Of Subfossil Macronycteris Spp. (Chiroptera: Hipposideridae) From Madagascar, Jamie Lynn Alumbaugh

Graduate Research Theses & Dissertations

Macronycteris bats are morphologically conservative between species but demonstrate intraspecific morphological variation between geographic locations and sexes. Two of the four living species of Macronycteris are found on Madagascar, where they are broadly distributed and demonstrate a trend in body size correlated with the latitudinal precipitation cline on the western side of the island. The presence of an extinct species, M. besaoaka, from Anhjohibe Cave in northern Madagascar suggests that Macronycteris was once more diverse, at least with respect to morphology. Since its description, taxonomic and phylogenetic revisions have reshaped our understanding of this genus. On Madagascar, these include the …

Comparative Tumorigenic Amino Acid Starvation And Integrated Stress Responses In Epithelial Vs. Mesenchymal Hcc Cells With Asct2 Or Lat1 Transporter Knockouts, Stephanie Lois Cooper Woodin

Graduate Research Theses & Dissertations

The previously established roles of the amino acid glutamine have recently been expanded to include not only protein and nucleotide synthesis, as well as contributing significantly to energy production via mitochondrial hydrolysis of glutamine to glutamate, but also the role of this molecule in the reductive carboxylation of α-ketoglutarate resulting in carbon atoms used in cell membrane biosynthesis. Additionally, glutamine participates in maintaining glutathione homeostasis as well as regulation of anabolic signaling. Given its central role in anabolic processes, glutamine is preferentially transported into human cancer cells at a significant rate as compared to normal cells. This distinction has prompted …

The Role Of Rmta In The Opportunistic Pathaogen Aspergillus Fumigatus, Roshan Dahal

Graduate Research Theses & Dissertations

The fungus Aspergillus fumigatus is an opportunistic human pathogen capable of causing

a life-threatening systemic lung infection known as invasive aspergillosis among immune compromised

patients. This group includes individuals infected with HIV, people with hematological

malignancies, solid organ transplant patients, persons with genetic immunodeficiency and cancer

patient undergoing chemotherapy. Due to the medical relevance of this organism, it is imperative

to discover novel genetic elements to design antifungal drugs against A. fumigatus dissemination,

virulence and survival during human infection. Previously, the putative arginine methyltransferase

gene rmtA was characterized in the model organism Aspergillus nidulans and the opportunistic

plant pathogen Aspergillus …

Practical Bioinformatics Applications In Microbes, Sarah Anne Entwistle

Graduate Research Theses & Dissertations

This thesis examines three methodologies for applying bioinformatics techniques to microbial data. A computational analysis of RNA sequencing data showed that hbx1 is a global regulator in Aspergillus flavus. A particular focus of this analysis is the characterization of hbx1 in regard to regulation of secondary metabolite gene clusters and their production of aflatoxins. An analysis of bacterial genomes identified ORFans in both pathogenic and non-pathogenic genomes, along with their presence in sequence features. This investigation demonstrated that ORFan genes shared by pathogenic genomes were more related to bacterial pathogenicity. Evaluation of multiple types of recombination events in RNA sequencing …

Dung Beetle Functional Traits Related To Restoration Management Practices In Tallgrass Prairie, Sheryl C. Hosler

Graduate Research Theses & Dissertations

The restoration of degraded ecosystems often focuses on reestablishing species richness and diversity of native organisms, especially plants. However, functional trait descriptions of communities are emerging as a more comprehensive approach to evaluating restorations, including consumer communities. I examined the species composition and functional traits of dung beetle (Coleoptera: Scarabeidae) communities across a chronosequence of restored tallgrass prairie in Illinois, in which sites varied in the presence of bison and the application of prescribed fire during the study. Data were collected on behavioral guild and body measurements, and functional diversity metrics (functional richness, evenness, divergence, and dispersion) were analyzed using …

How Coping Process Relates To Eating Behaviors Among Military Veterans With Posttraumatic Stress Disorder, Debra Kimberlin

Graduate Research Theses & Dissertations

Posttraumatic stress disorder (PTSD) is a mental health condition faced by many veterans, and veterans with PTSD have higher rates of poor health and obesity than civilians. Evidence suggests mental health issues such as PTSD may be associated with disordered eating behaviors. In addition, veterans with PTSD may select a variety of coping behaviors to manage stress and emotions, some of which may negatively impact their health. This study explored the relationships between coping process, emotion- and stress-related eating, and eating disorder risk among a sample of veterans with PTSD (n = 239) who were recruited through veteran service organizations …

Stress Response And Performance Changes Of Law Enforcement Officers’ Marksmanship Under Varied Levels Of Stress, Brandon Michael Male

Graduate Research Theses & Dissertations

Objective: To investigate changes in physiological stress response and shooting performance of law enforcement officers under varying levels of stress. More specifically, the purpose of this study was to determine how increasing levels of stress from operating a firearm on static targets changes when law enforcement officers are subjected to a simulated life-threatening or dangerous situation.

Background: Performance changes associated with high levels of stress have been well documented in disciplines such as sports psychology, medicine, and military/law enforcement fields. However, little work has been done to draw comparisons between firearm training methods and qualification procedures which closely mimic armed …

Body Image Dissatisfaction And Disordered Eating Behaviors Among Niu Students, Sara Mattillion

Graduate Research Theses & Dissertations

The purpose of this study was to determine if there was a significant difference in the presence of body image dissatisfaction and disordered eating among NIU college students.

The objectives of the study included: determining if there was a significant difference in the presence of body image dissatisfaction among NIU college students, determine if there was a significant difference in the presence of disordered eating among NIU college students, and to determine if there is a relationship between body image dissatisfaction and disordered eating among NIU college students.

The transition into college and graduate school is challenging and can induce …

Development Of The Adept Screening Tool To Identify Health And Fitness Apps That Pose Risk For Individuals Susceptible To Disordered Eating, Georgia Mcartney

Graduate Research Theses & Dissertations

Popular health apps could contain risk factors for disordered eating such as restriction, weight stigma, harmful exercise, or compensation. Although previous studies have examined the connection between social media and disordered eating, data is lacking regarding risk factors from health apps. The purpose of this study is to develop a rating tool to detect, examine, and categorize app-based risk factors for disordered eating.

This study utilized a comprehensive review of literature regarding risk factors for disordered eating, connections between app use and disordered eating behaviors, and existing technology rating tools. Based on this framework, the Awareness of Disordered Eating

Promotion …

Microbiome And Metagenome Of An Anthropogenic Hyperalkaline Slag-Fill Waterway In South Chicago, Illinois, Usa, Jan Ingemar Ohlsson

Graduate Research Theses & Dissertations

The aquifers and waterways around the Lake Calumet area of south Chicago, IL, USA have been affected by a century of intensive industrial waste dumping. Steel slag in particular was used as infilling to claim most of the Calumet Wetlands for industrial and residential development. Since the prohibition and cessation of these disposal activities, a growing body of research has recorded the environmental and health effects of the infill, particularly where interactions between slag minerals and rainwater produces extremely alkaline groundwater (pH ≥13). Atmospheric carbon dioxide interacting with these calcium hydroxide-rich slag fluids produces massive precipitation of a loose, white …

Television Viewing And Markers Of Physical Fitness And Body Composition In College-Aged Students, James Peterson

Graduate Research Theses & Dissertations

Television viewing is one of the most popular sedentary activities in America. Sedentary behavior has been linked to increased risk of obesity, hypertension, cardiovascular disease, and all-cause mortalities. While many studies have focused on associations in children and middle aged to older adults, there is a dearth of literature regarding young college aged students. PURPOSE: The purpose of this study was to examine the association between daily television screen time viewing and markers of physical fitness and body composition in young college aged students. METHODS: The sample population consisted of 141 Northern Illinois University young college aged students. Subjects completed …

Prevalence Of Condensed Tannins In The Diet And Its Impact On Protein Intake Of Diademed Sifakas (Propithecus Diadema) At Tsinjoarivo, Madagascar, Emma Grace Thurau

Graduate Research Theses & Dissertations

Plant secondary metabolites (PSMs) are compounds found in plant tissues designed to deter herbivores. Tannins, a type of PSM, can bind to proteins and reduce their availability to consumers. The detoxification limitation hypothesis suggests herbivores will prefer foods with lower amounts of PSMs and exhibit decreasing rates of feeding when PSM levels are high in order to stabilize the amount of PSMs ingested. Although this hypothesis has been supported by observations from other mammalian groups, it has not been tested in lemurs with regards to their consumption of tannins.

I observed diademed sifakas (Propithecus diadema) over a 2-month period (June-August …

Mapping The Effects Of 5’ Untranslated Region Deletions On Translation Of 3a In Bmv Rna3, Courtney Marie Bozman

Graduate Research Theses & Dissertations

Brome mosaic virus (BMV) is a positive sense, single-stranded RNA virus that infects a wide array of plant hosts globally including: wheat, oats, maize, barley, and rye. Since its discovery in the 1940s, BMV has served as a model organism in uncovering the modalities of viral infectivity, composition, translation, and replication. In many viruses, it is typically imperative to recruit cellular machinery to produce viral proteins as their coding capacity is limited. Therefore, there are sequences and structures within the leader sequences allow them to be competitive with the eukaryotic mRNAs. Although BMV has been thoroughly researched in these other …

Unmanned Aerial-Vehicle-Based Measurement Of Urban Forests, Earle W. Isibue

Graduate Research Theses & Dissertations

Human and natural forces continually act on urban forests to producing changes that leading to trees being removed or replanted. Therefore, to manage the urban forest effectively, periodic inventories are needed to ensure that information about the urban forest is current and comprehensive. This task has traditionally been accomplished by manual ground-based field surveys, or more recently by lidar; however, these methods are expensive, either in terms of time, labor, or cost. This project proposes a novel method of using Unmanned Aerial Vehicles (UAVs) to more accurately measure urban tree height and trunk diameter than is currently possible using conventional …

Evaluating Spatial Distributions Of Scent-Marks In Semi-Free-Ranging Groups Of Lemur Catta At The Duke Lemur Center, Shallu Prasher

Graduate Research Theses & Dissertations

Scent-marking is a widespread form of olfactory signaling exhibited in mammals and for a select group of primates. Although research exists on the process of scent-marking, surface preferences, and for the function of scent-marking in lemurs, analyses of how lemur group scent-marking distributions map onto their home range are lacking. The endangered ring-tailed lemur (Lemur catta) deposits scent-marks using its ano-genital, chest, and wrist glands. This project was designed to collect data on two groups of semi-free-ranging captive ring-tailed lemurs at the Duke Lemur Center to first assess how scent-marks are distributed within their home ranges; and second, to evaluate …

Seabird Threats On Land And At Sea: Measuring Nearshore Response To Invasive Predator Eradications And Storm Petrel Diet Response To Fishing Expansion, Lyndsay L. Rankin

Graduate Research Theses & Dissertations

Seabirds are crucial components of many marine and island ecosystems, and yet they are the world’s most threatened group of birds. They spend most of their lives at sea, where they face numerous threats including climate change, pollution, and fisheries. When they return to land to breed, they are threatened by human disturbance and invasive species. This research examines seabird response to threats on land and at sea using stable isotope analysis. Seabirds provide multiple ecosystem services, such as depositing rich marine-derived nutrients into the islands where they nest. Invasive predators disrupt this bottom-up control by decimating seabird populations. Successful …

Study Of Genetic Regulators That Control Development And Secondary Metabolism In The Genus Aspergillus, Timothy Satterlee

Graduate Research Theses & Dissertations

In this study, I investigated the role of two different regulatory genes in two different species of pathogenic fungi from the genus Aspergillus. The first study involves a transcriptome analysis of the epigenetic regulator rmtA in the plant pathogen Aspergillus flavus. A. flavus colonizes numerous oil seed crops such as corn, peanuts, treenuts and cotton worldwide, contaminating them with aflatoxins and other harmful potent toxins. Previously our lab characterized the gene rmtA, which encodes an arginine methyltransferase in A. flavus, and demonstrated its role as regulator of the expression of the aflatoxin gene cluster and concomitant synthesis of toxin. Furthermore, …

Sensory And Experiential Characteristics Of Food Rejection By Adult Selective Eaters, Kim O. Milano

Graduate Research Theses & Dissertations

Selective or picky eating in adults is an eating behavior that is characterized by the restriction of a significant number or variety of foods, particularly fruits and vegetables. It is also associated with poor appetite and fear of eating, and if severe enough may be classified as Avoidant Restrictive Food Intake Disorder (ARFID). Research is limited regarding the differences in food preferences and reasons for food rejection by adult selective eaters, with and without other restrictive eating behaviors. Previous research suggests there may be differences in the types of food restricted due to selectivity, versus poor appetite and fear of …

Functions Of Dfrp And Other Drg Pathway Genes In The Moss Physcomitrella Patens, Robert James Srygler

Graduate Research Theses & Dissertations

GTP binding proteins (G proteins) are molecular switches that regulate many aspects of physiology in all cells. DRG1 and DRG2 (Developmentally Regulated GTP-binding proteins) have been found in all eukaryotes examined. Amino acid identity across eukaryotes is about 65% within an orthologous group and 55% between paralogs of a single species. Although DRGs are likely involved in some aspect of translation regulation, details of their precise cellular functions are not known.

In addition to DRGs there are other genes/proteins that comprise the “DRG pathway.” DFRPs (DRG Family Regulatory Proteins) have been shown to physically and genetically interact with DRGs. Previous …

Biochemical And Biophysical Studies Of Vision Loss: Cataract And Macular Degeneration, Michael C. Vega

Graduate Research Theses & Dissertations

Age-related biochemical and biophysical changes to the structural properties of ocular lens membrane proteins and retinal pigment epithelium (RPE) cells are thought to play an underlying role in the etiology and progression of age-related cataract and macular degeneration. This dissertation work studies biophysical properties of biomimetic membranes to aid in further understanding of the role of aquaporin-0 in cataract pathogenesis and the biochemical composition of age-related pigmented granules, melanolipofuscin, and their implications in macular degeneration.

Cataract is the leading cause of blindness globally and is characterized by the opacification of the ocular lens. As a key optical component of the …

Controlling Lonicera Maackii (Amur Honeysuckle): Basal Bark And Prescribed Fire Efficacy And Impacts, Kaleb Baker

Graduate Research Theses & Dissertations

Lonicera maackii is a non-native shrub that has invaded eastern and midwestern North American deciduous forests, altering the ecosystem functions and reducing biodiversity. Managers tasked with controlling L. maackii, being resource limited, require effective methods that are quick and easy to use without inflicting extensive nontarget damage. This study explores prescribed fire and seasonal basal applications of triclopyr as control methods and examines their extent of off-target damage. Paired-split plots were established to implement seasonal basal bark treatments within burned/unburned units where individual L. maackii were tracked to determine mortality and the hyperlocal impacts of management. Basal bark treatments were …

Validation Of A Low-Cost Sensor For Development Of Wearable And Handheld Spectrometers, Justin Leslie Cathey

Graduate Research Theses & Dissertations

This study examined the possibility of creating a quantitative radiometric spectrometer from a low-cost relative radiometric spectrometer sensor through the use of statistical analysis and development of a correction factor (p > 0.05). Radiometric spectrometers are able to measure and fully characterize the radiometric and photometric characteristics of light, while relative spectrometers are not able to fully, quantitatively characterize the radiometric response from a light source, thus they are less expensive. The spectrometer created in this research is able to measure and produce comparative lighting parameters as well as an actual radiometer spectrometer.

The photoreceptors cells within the human eye allow …

Growth And Survival Of Wild And Head-Started Blanding’S Turtles (Emydoidea Blandingii), Callie Klatt Golba

Graduate Research Theses & Dissertations

Blanding’s Turtles (IUCN Endangered) are long-lived reptiles with delayed sexual maturity. Anthropogenic landscape changes have increased threats to juvenile turtles, resulting in unnaturally low recruitment. Head-starting has become a popular conservation strategy that aims to increase juvenile recruitment by avoiding the increased predation of the vulnerable nest and hatchling age-class. However, there is still debate about whether or not it is an effective management tool. Assessments of head-starting are becoming more prevalent, but long-term studies are needed to critically evaluate the success of such interventions. In particular, information is needed on how head-starts fare compared to wild-born turtles. The Lake …

The Risk For Developing Eating Disorders Among Indivdiuals With Diabetes, Nicole Havlik

Graduate Research Theses & Dissertations

Objective: The objective and purpose of this research was to determine if individuals with type 1 and type 2 diabetes are at risk for developing eating disorders. Currently, there is a gap in literature on the topic of eating disorders and diabetes. Further, there is little research on those with type 2 diabetes and their risk for eating disorder development. Management of diabetes and eating disorder treatment contradict each other. Therefore, there is a need to understand the risk involved in the treatment of diabetes in order to prevent eating disorder development.

Methods: The current study used a Likert-type scale …

Effects Of Habitat Disturbance On The Teeth Of Propithecus Diadema In The Forests Of Tsinjoarivo, Madagascar: Implications For Species Viability And Conservation, Katie Susann Heffernan

Graduate Research Theses & Dissertations

The accelerated transformation of Madagascar’s ecosystems is a direct result of habitat destruction which has devastating consequences for the viability of the island’s animals. This dissertation focuses on the critically endangered diademed sifaka, Propithecus diadema, living in the rainforests of Tsinjoarivo, Madagascar. Tsinjoarivo has been heavily impacted by slash and burn agriculture and transformed into isolated forest fragments. Previous research by Irwin and colleagues has demonstrated that the diet and group dynamics of Propithecus diadema differ between continuous and degraded forests, but whether fragmented landscapes provide tougher foods and lead to behavioral changes through increased tooth wear has not been …

Eastern Tallgrass Prairie Management And Its Impact On Grassland Birds, Heather Herakovich

Graduate Research Theses & Dissertations

The majority of tallgrass prairie has been converted to agriculture over the past two centuries, making it one of the most threatened ecosystems globally. Agricultural conversion of prairie fragmented the landscape, causing declines and local extirpation of many grassland birds. Restoration projects have sought to increase the quality and size of prairie fragments by converting cultivated land back to prairie through revegetation and management with prescribed fire, hypothetically increasing breeding habitat for grassland birds. Bison and other grazers are now being reintroduced to prairie restorations as a final step in the restoration process to increase habitat heterogeneity.

The goal of …

Effects Of Different Dual Tasking Modalities On The Standing Balance Performance Of College Students, John Patrick Manning

Graduate Research Theses & Dissertations

Center of pressure (CoP) and related-metrics are widely accepted indicators of balance performance. Traditional indices of balance performance (e.g. sway area) have been seen as incapable of fully characterizing CoP dynamics, therefore newer measures have been used in contemporary literature to characterize regularity, complexity, and dimensionality of CoP signals. A popular method of increasing standing task difficulty is through the addition of a cognitive task, referred to as dual-task. PURPOSE: The present study sought to investigate the potential of a hierarchical attentional difficulty within the dual-task paradigm and to propose way to order those difficulties by examining differences in static …