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Full-Text Articles in Life Sciences
Occurrence, Antibiotic Resistance, And Survival Of Fecal Enterococci In Turkey Litter, Steven Glynn Mcbride Ii
Occurrence, Antibiotic Resistance, And Survival Of Fecal Enterococci In Turkey Litter, Steven Glynn Mcbride Ii
Masters Theses, 2010-2019
The United States Environmental Protection Agency’s National Water Quality Inventory and the Commonwealth of Virginia’s 305(b)/303(d) Water Quality Assessment Integrated Report show fecal bacteria to be the most common cause of impairment for both streams and estuaries. Human and animal sources have both been identified as significant contributors of pathogenic bacteria to surface waters. In this study, turkey litter from a farm in Shenandoah County, VA was surveyed for total culturable bacteria and total culturable enterococci before and after a transition to organic rearing practices. The enterococci were identified to species phenotypically using the Biolog Microbial Identification System and resistance …
Isolation And Enumeration Of Vibrio Vulnificus And Vibrio Parahaemolyticus From Coastal Virginia, Zackary A. Zayakosky
Isolation And Enumeration Of Vibrio Vulnificus And Vibrio Parahaemolyticus From Coastal Virginia, Zackary A. Zayakosky
Senior Honors Projects, 2010-2019
Vibrio vulnificus and V. parahaemolyticus are gram-negative, halophilic bacteria that are found throughout estuarial waters during the summer months, and are commonly associated with human infection. Gastroenteritis and other related symptoms can occur following infection from either organism, which most often occurs as a result of consumption of raw oysters or other seafood. V. vulnificus is particularly virulent, and can also produce wound infections that lead to severe septicemia and death. Due to the increasing rates of infection for these two organisms, recent research efforts have focused on potential environmental conditions and reservoirs that would be indicative of increased Vibrio …
Next-Generation Sequencing Of A Multi-Drug Resistance Plasmid Captured From Stream Sediment, Kevin G. Libuit
Next-Generation Sequencing Of A Multi-Drug Resistance Plasmid Captured From Stream Sediment, Kevin G. Libuit
Masters Theses, 2010-2019
Plasmids in agriculturally-impacted bodies of water may play a significant role in the dissemination of antibiotic resistance. Previously, Erika Gehr, as part of her M.S. thesis work in our laboratory, captured environmental plasmids without cultivation of host bacteria from stream sediment into Escherichia coli. Individual plasmids were capable of conferring resistance to a surprising array of antibiotics including aminoglycosides and extended-spectrum β-lactams. In this study, we developed a method to sequence multi-drug resistance plasmids using both Oxford Nanopore MinION and Ion Torrent Personal Genome Machine sequencers. Plasmid pEG1-1 was sequenced on both platforms and a hybrid assembly utilizing data …
The Potential For Replication And Transmission Of Antibiotic Resistance Plasmids In An E. Coli Population In Agriculturally Impacted Stream Sediment, Erika Gehr
Masters Theses, 2010-2019
The use of antibiotics in agriculture is thought to be a major cause of resistance in microorganisms found in the environment. Horizontal transfer of genetic information from transient to native and from native to transient bacterial populations may enhance the spread and recombination of resistance genes and might play a role in the formation of multi-resistant organisms in environmental reservoirs. Tetracycline resistance plasmids were compared using three isolation techniques -- traditional “endogenous” extraction from isolates, “exogenous” plasmid capture, and direct plasmid extraction from sediment --to determine the potential for plasmid born resistance in an E. coli population found in agriculturally-impacted …