Life Sciences Commons^™

Influence Of Testosterone On Male-Male Competition In The Red-Sided Garter Snake, Thamnophis Sirtalis Parietalis, Isabella M. G. Bukovich

Senior Honors Projects, 2020-current

Female mimicry in the red-sided garter snake, Thamnophis sirtalis parietalis, is described in several well-studied evolutionary advantages, but an aspect that remains elusive is how reproductive traits are linked to the 3-fold higher circulating androgens in males with this phenotype. In this study, we implanted male garter snakes (n=15 per group) with either a blank implant (SHAM; control) or a T implant, the latter simulating the female mimic phenotype. Following simulated low-temperature dormancy, males were scored for courtship intensity over three days of behavioral trials with females housed in the same facility. Males were sacrificed, and sperm were collected …

Efficacy Of Pulmonary Ultrasound Compared To N-Terminal Prohormone Brain Natriuretic Peptide As A Diagnostic Tool For Congestive Heart Failure In Patients Presenting With Acute Dyspnea In The Emergency Setting, Leah Krohn, Michael Burns

Physician Assistant Capstones, 2020-current

Objective: To determine the efficacy of using pulmonary ultrasound as a diagnostic tool in acute dyspnea of undetermined cause compared to the use of serum N-Terminal prohormone Brain Natriuretic Peptide (NT-proBNP) in the diagnosis of heart failure.

Design: Systematic literature review

Methods: Searches were conducted in PubMed and Scopus using the terms pulmonary ultrasound and congestive heart failure and studies within the last 10 years. Studies that used pulmonary ultrasound compared to BNP as a marker for the diagnosis of heart failure were included.

Results: All three studies found that the use of pulmonary ultrasound was a more specific …

The Effects Of Exercise On Breastmilk Composition And Supply: A Critical Literature Review, Jewell Mcroy

Masters Theses, 2020-current

It is well established that breastfeeding provides dual benefits to both the mother and the infant and is the preferred method of providing nutrients to infants essential for growth and development. Participating in regular physical exercise is also well-supported by research to be beneficial to one’s overall health and quality of life. Currently there are no physical activity guidelines developed for women who are postpartum and breastfeeding. An online database search was conducted; three articles met the inclusion criteria and were included within the review. Studies were included if there were direct measures of human breastmilk composition, supply/volume, and included …

Therapeutic Potential Of A Ketogenic Diet In The Treatment Of Major Depressive Disorder, Jordan A. Murrin

Masters Theses, 2020-current

Major depressive disorder (MDD) is the second most common mental health condition and a leading cause of disability in the world. It is theorized that MDD develops from a combination of biological, psychological, and social stressors. The condition is typically treated using pharmaceuticals and psychotherapy. However, not all individuals with MDD have access to or choose to use these treatments, or may prefer to incorporate therapeutic lifestyle changes such as exercise, sleep, and healthy eating. Even with treatment, MDD can alter brain structure and function, leading to the development of comorbid mental health and chronic metabolic conditions like obesity, cardiovascular …

Isolation, Enumeration And Antibiotic Profiling Of Vibrio Vulnificus And V. Parahaemolyticus From Coastal Virginia, Stanley E. Peyton Jr.

Masters Theses, 2020-current

Vibrio vulnificus and V. parahaemolyticus are gram-negative, halophilic bacteria that reside in estuarine waters and are associated with infections in humans. These bacteria can cause gastroenteritis through their presence in raw fish and shellfish consumed by humans. V. vulnificus can also produce wound infections leading to severe septicemia, and in some cases, death if not treated promptly. With increasing incidence of infections due to these two organisms, research efforts have focused on potential reservoirs and environmental conditions that can increase human exposure to, and infection, with these species of bacteria. This study was conducted in order to examine the role …

Population Status, Threats, And Conservation Of Preuss's Red Colobus (Piliocolobus Preussi) And Other Diurnal Primates In The Ndokbou Forest, Littoral Region, Cameroon, Ruth Bowers-Sword

Masters Theses, 2020-current

The Ebo-Makombe-Ndokbou forest block in southwest Cameroon lies within the Gulf of Guinea biodiversity hotspot, characterized by extremely high levels of species richness and endemism, including those of primates. These forests may contain one of the last populations of the Critically Endangered Preuss’s red colobus monkey (Piliocolobus preussi; PRC), which is found only in southeastern Nigeria and western Cameroon. Gun hunting for bushmeat and habitat loss and degradation from logging and agriculture are the main threats to PRC. The conservation status of PRC and other primates in the Ndokbou forest are largely unknown, with most regional research efforts occurring in …

Impact Of 12-Weeks Of Nordic Pole Walking On Arterial Stiffness In Sedentary Overweight And Obese Adults, Arbër N. Shehu

Masters Theses, 2020-current

Arterial stiffness (AS) is an independent predictor for cardiovascular disease morbidity and mortality. Regular aerobic exercise is considered to improve AS. Nordic walking provides important health benefits, however, the effect of this walking method as treatment for AS remains unclear. We conducted a randomized controlled study to examine the effect of a 12-week supervised group walking intervention (controlled pace, ~3000 steps/day, 30 minutes/day, 5 times/week), with (NW) and without (SW) Nordic poles, on AS in sedentary overweight and obese adults. Fourteen individuals were randomly assigned to NW (n=7; median age 54.0 years; body mass index (BMI)=31.3 kg/m²) or …

Whole Genome Sequence Analysis Of A Transmissible Multidrug-Resistance Plasmid Captured Without Cultivation From Poultry Litter, Emma C. Eisemann

Senior Honors Projects, 2020-current

Use of antibiotics in the agricultural industry introduces selective pressure and, consequently, could increase the presence of antibiotic resistant organisms in surrounding environments. One such environment is litter (manure and bedding) produced during large-scale poultry production in the Shenandoah Valley. Litter, with its microorganisms, is commonly applied to fields within the Shenandoah River watershed. Antibiotic resistance (AR) and virulence genes are potentially transmissible between organisms through horizontal gene transfer of genetic mobile elements, for which poultry litter could be a reservoir. The typical, culture-based approach to detecting and analyzing AR plasmids and other mobile genetic elements is limited due to …

Investigation Of Potentially Catalytic Residues Of Uba5 Through Mutagenesis, Purification, And Structural Characterization, Grant Bradley

Senior Honors Projects, 2020-current

Ubiquitin-fold modifier 1 (Ufm1) is a member of the Ubiquitin (Ub) family of proteins whose primary function is degradation of proteins through a sequential mechanism of chemical reactions. Though Ufm1’s specific roles are largely unknown, this family of proteins has shown to play a part in a wide variety of processes, including regulation of the cell cycle¹, secretory functions of cells^2,3, and blood clotting⁴. Ufm1’s mechanism of action proceeds with the aid of three enzymes: an E1, E2, and E3. Uba5 is the E1 activating enzyme that is specific to Ufm1, and its mechanism of …

The Function Of The Intrinsic Foot Muscles During Turns And Mediolateral Shifts In Balance, Hope E. Kircher

Senior Honors Projects, 2020-current

Purpose: Atrophy of the intrinsic foot muscles (IFM) has been associated with foot deformity, balance deficiencies, and plantar ulceration in patients with diabetes. Nevertheless, little is understood regarding the function of the intrinsic foot muscles responsible for abduction of the toes, and no study has directly assessed the role of the abductors in maintaining mediolateral balance or foot alignment and integrity during turning and other nonlinear activities. The purpose of this study was to determine the activation levels of abductor hallucis (ABDH), abductor digiti minimi (ABDM), the first (PDIO1) and third (PDIO3) dorsal interossei, and flexor digitorum brevis (FDB) during …

Placebo-Induced Changes In Force Production For Types Of Muscle Contractions, Nicholas V. Antonacci

Senior Honors Projects, 2020-current

Purpose: To test the impact of a placebo drink on acute force production during isometric and isokinetic leg extensions in male and female college students. Methods: Nine male and five female subjects apparently healthy and free of leg injury completed familiarization testing and two counterbalanced trials. In one trial, participants were told they were consuming a performance-enhancing drink, although the drink contained only flavoring. In the other trial, participants were not given any drink (control). Both trials then included concentric and eccentric strength tests performed at 60 degrees per second, and isometric strength tests with the knee at a 70 …

Relationship Between Muscular Strength And Balance In An Elderly Population, Mikayla N. Basil

Senior Honors Projects, 2020-current

Purpose: To examine the association between grip strength, leg strength and balance test performance in elderly individuals.Methods: Fourmales and four females (average age = 72.8 ± 2.1 years, average height = 167.8 ± 9.4 cm, average weight = 74.8 ± 14.4 kg) participated in this study. Participants were administered the Berg Balance Test and assigned a score out of 56. They then performed a maximal grip strength test, and maximal isokinetic leg extension and flexion tests on their dominant leg to obtain peak torque. Correlations were calculated between all testing variables using Pearson correlation coefficients.Results: No significant correlations …

Characterizing The Role Of Β-Amylase3 In Cold Stress Response And Recovery In Arabidopsis Thaliana, Isabelle G. Houston

Senior Honors Projects, 2020-current

Starch is a polymer of glucose that is used as an energy store in plants. Mobilization of starch has implications in abiotic stress survival and recovery. While the importance of carbon and energy allocation in plant survival has been explored, the specific roles of starch degrading enzymes in plant responses to stress are still unclear. β-Amylase3, or BAM3, is the principle starch degrading enzyme at night and is transcriptionally upregulated in response to cold stress in the plant Arabidopsis thaliana. Using single and quadruple knockout mutant plants, I aimed to clarify the role of BAM3 in the response to …

Effect Of Ambient Temperature On Recovery Of Surgically Instrumented Sprague-Dawley Rats, Gianna Mangone

Senior Honors Projects, 2020-current

Based on current guidelines for housing rodents after surgical instrumentation, rodents may be housed at temperatures that hinder surgical recovery and cause thermal stress– room temperature (21°C) being one of them. Rodents are often housed at room temperature since this temperature is easy for humans to be caring for them. However, if recovering rodents are thermally stressed, experimental results will be confounded. To address this issue, Sprague-Dawley rats were surgically instrumented with radiotelemtry thermoprobes (Data Sciences, #TA-10F40) to monitor their core temperatures, then housed at one of five temperatures to assess the effect ambient temperature had on multiple aspects of …

Novel Method To Determine Paleodiet Of Extinct Equid Merychippus Sp. Using Dental Calculus, Ranjit Z. S. Virk

Senior Honors Projects, 2020-current

Within the Miocene Epoch, the emergence of grasslands within North America coincided with the incidence of higher-crowned teeth (hypsodonty) within the proto-horse Merychippus that allowed for the inclusion of these expanding grasslands as a food source. As herbivorous paleofauna consume plant matter, microscopic remains become incorporated within dental calculus and, due to their diagnostic morphology, can be used to identify dietary components. In Merychippus, the recovery of plant microfossils holds the potential to provide greater clarity on the paleodiet of these equids. In the present study, the purpose was to quantify and compare Merychippus paleodiet constituents among sample groups. …

Do Innexins Function In The Extreme Cold Response Of Drosophila Melanogaster, Madison A. Ward

Masters Theses, 2020-current

Nociception is an organism’s ability to detect, process and reflexively respond to potentially damaging stimuli. While the process of nociception has clear, protective advantages, inappropriate and prolonged signaling can lead to chronic pain in humans. Nociception is a vital and genetically conserved process, thus cold nociception in Drosophilaprovides a model for identifying molecular components required for nociceptor function in vertebrates. Drosophila Class III dendritic arborization (da) neurons have previously been shown to be involved in the cold nociceptive response. Due to the importance of fast response to damaging stimuli, we hypothesize that electrical synapses are involved in cold nociception. …

The Impact Of Acute Exercise On Factor Viii In Young E-Cigarette Smokers, Andrew R. Allen

Masters Theses, 2020-current

Abstract

Background: There is a strong association between factor VIII (FVIII) and presence of cardiovascular disease (CVD). Exercise is a known trigger for myocardial infarction, and the majority of ischemic events from physical stress occur due to an occlusive thrombus. FVIII increases acutely with exercise and remains elevated hours after exercise. Furthermore, FVIII is also higher in cigarette smokers, however, it has not been investigated in e-cigarette smokers. The purpose of this study is to identify potential differences in FVIII before exercise, after exercise, and its magnitude of change in e-cigarette users versus non-users.

Methods: Eighteen individuals (8 …

Habitat Utilization And Impact Of Flooding On James Spinymussel (Parvaspina Collina) Populations In Virginia Streams, Christine A. Verdream

Masters Theses, 2020-current

The James spinymussel (Parvaspina collina) is Critically Endangered and endemic to Virginia’s James River basin. P. collina was listed as Endangered in 1988 and more than 90% of the species has been lost over the last 30 years (USFWS 1990). Despite the development of a recovery plan in 1990, there has not been a comprehensive reassessment of P. collina in over 30 years. This study explored the relationship between flooding and P. collina population dynamics in a flood prone stream and further explained habitat occupancy. Study sites included Swift Run and Little Oregon Creek, both of which still …

Modeling Species Distribution And Habitat Suitability Of American Ginseng (Panax Quinquefolius) In Virginia, Jacob D. J. Peters

Masters Theses, 2020-current

American ginseng (Panax quinquefolius) is a well-known and sought-after medicinal plant native to North America that is facing increased threat of extinction due to overharvesting, herbivory, and habitat loss. Species distribution and habitat suitability models may be valuable to landowners interested in sustainable harvest or to institutions interested in the conservation and restoration of the species. With unequal sampling efforts across a region of interest, it is likely that some locations with appropriate habitat may be misrepresented in model predictions. This study refined a state-derived species distribution model for ginseng through increased sampling effort across the Cumberland Plateau …

Antibacterial Properties Of Novel Amphiphiles, Ariana Simeone

Masters Theses, 2020-current

Hospital acquired infections, particularly antibiotic resistant infections, are a growing concern due to their infection and mortality rate, as well as their growing economic burden, and it has been estimated that by the year 2050 the associated mortality rate will be around 10 million deaths per year. A solution to this is better means of prevention, and amphiphiles, compounds with at least one hydrophilic head group and at least one hydrophobic tail, are promising candidates for stopping the spread of infectious bacteria. Novel amphiphiles with two bipyridinium head groups, separated by a hydrophobic carbon spacer of varying lengths, and each …

Research And Development Of A Computational Simulation Of A Rat Tail, Abed Adawi

Masters Theses, 2020-current

To better understand the potential use of synergies in the neural control of muscle in the rat tail during the NWR, a new computational biomechanical model of the tail was created in Matlab/Simulink based on experimentally measured parameters – length, mass, stiffness, and damping - of the tail. The tail was simplified from 28 coccygeal vertebrae to 12 equal length segments. The model was validated by applying known forces to the tail and comparing the observed and predicted movements. Using forward dynamics assisted data tracking (FDADT), we determined that while a single tendon synergy was adequate for small and proximal …

Market To Table: A Physical Activity And Healthful Eating Approach To Diabetes Prevention, Alison Schwartz

Masters Theses, 2020-current

According to the Center of Disease Control and Prevention (2019), one out of three US adults has prediabetes and healthful eating and physical activity can help to prevent or delay the onset of Type 2 diabetes. This study examines physical activity and healthful eating behavior following a 6-week Market to Table intervention that includes an experiential workshop series among participants who have prediabetes or are at risk for prediabetes. Current participants or graduates of the Sentara RMH Medical Center Diabetes Prevention Program and James Madison University faculty or staff who had been diagnosed with prediabetes or are at risk were …

Effects Of Unilateral And Bilateral Squatting On Rotational Speed In Male And Female Collegiate Golfers, Emmett Van Der Snick

Masters Theses, 2020-current

Resistance training in combination with plyometrics and medicine ball routines has been shown to improve rotational speed and power. One area that has not been observed has been the difference between bilateral squatting and unilateral squatting on rotational speed. The purpose of the study was to observe the effects of unilateral and bilateral squatting on club head speed in collegiate golfers. Eight male and nine female collegiate golfers were randomly assigned to a unilateral squat group which was the experimental and a bilateral squat group which was the control group and participated in twelve training sessions over the course of …

Determining The Impacts Of Urbanization On Song Structure And Its Function In Territorial Defense For Gray Catbirds (Dumetella Carolinensis), Morgan L. Rhodes

Masters Theses, 2020-current

In urbanized habitats, animals are faced with novel selection pressures such as differences in community structure and increased urban noise. Urban noise pollution can negatively impact songbirds as low-frequency noise often masks portions of birds’ mating signal and reduces signal transmission. Previous research has demonstrated that songs of birds in more urban habitats have structural differences that enhance signal transmission when noise is present. These studies have focused on species that deliver short, stereotyped songs with limited repertoires. Gray catbirds (Dumetella carolinensis, family: Mimidae) sing long bouts containing imitated, improvised, and invented song elements, and therefore may have …

The Impact Of E-Cigarette Smoking On Pulmonary Responses To Maximal Exercise, Chelsea N. Robinson

Masters Theses, 2020-current

Electronic cigarettes (e-cigarettes) have emerged as popular alternatives to smoking conventional tobacco, particularly in a younger demographic. However, there is emerging and conflicting evidence on the magnitude of airway damage with e-cigarette usage. While evaluating airway health can be challenging, using a stimulus such as exercise may be used to elucidate the effects of e-cigarettes on the pulmonary system. Purpose: To determine the impact of an acute maximal exercise on changes in pulmonary function (i.e. bronchodilation and bronchoconstriction) in young adult exclusive e-cigarette smokers (S) compared to nonsmokers (NS). We hypothesized that S will have lower post-exercise bronchodilation after an …

Splitting Up A Complex Mess: The Effectiveness Of Statistical Analysis On Delimiting Species Complexes, Sara N. Schoen

Masters Theses, 2020-current

Recent studies have highlighted a need for more refined tools in species delimitation. This is especially true when considering diversity within species complexes, where members are morphologically similar and traditional tools have thus far failed to provide clearly defined boundaries between species. This project seeks to refine our traditional tools of species delimitation and apply new tools to the challenges created by species complexes. The focus organisms of this study are the anurans of the Limnonectes kuhlii complex. This species complex comprises more than 25 species of stream frogs from Southeast Asia. Traditionally, morphometrics (particularly linear measures) has been the …

Microglia And Fractalkine Involvement In Shaping Multimodal Midbrain Circuitry, Cooper A. Brett

Masters Theses, 2020-current

Microglial cells (MGCs) are highly dynamic and have been implicated in shaping discrete neural maps in several systems. MGCs respond to numerous cues in their microenvironment, including a neuronally-expressed chemokine, CX3CL1 (fractalkine). The present study examines microglial and CX3CL1 patterns with regard to the emerging modular-extramodular framework within the lateral cortex of the inferior colliculus (LCIC). The LCIC is a shell region of the auditory midbrain where discrete compartments receive modality-specific connections, whereby somatosensory inputs terminate within modules and auditory inputs target surrounding extramodular zones. A reliable modular marker, glutamic acid decarboxylase (GAD), enables visualization of emerging modular domains in …

The Effect Of Varying Fatty Acid Composition In A High-Fat Meal And Its Impact On Postprandial Airway Inflammation, Breanna L. Davidson

Masters Theses, 2020-current

The Western Diet is typically high in saturated fats (SF) or omega-6 polyunsaturated fatty acids (O6FA) with insufficient amounts of omega-3 polyunsaturated fatty acids (O3FA). When chronic, this diet has been associated with an increased risk of respiratory diseases. PURPOSE: To examine the effect of varying the fatty acid composition of an acute High-Fat Meal (HFM) on postprandial airway inflammation. METHODS: Fifteen individuals [6 M, 9 F; body mass index (BMI) = 25.3 ± 6.6 kg/m²] consumed three HFM smoothies separated by at least 48 hours. The three smoothies were high in SF, O6FA, and O3FA …