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Isolation And Characterization Of An A4 Mycobacteriophage From Central Illinois, Aida `Cheung, Jadeah Spindler, Ruchen Tian, Jordan Miller, Audrey Smith, Meghan Linder, Tiffany Remijas, Richard Alvey, Faculty Advisor
Isolation And Characterization Of An A4 Mycobacteriophage From Central Illinois, Aida `Cheung, Jadeah Spindler, Ruchen Tian, Jordan Miller, Audrey Smith, Meghan Linder, Tiffany Remijas, Richard Alvey, Faculty Advisor
John Wesley Powell Student Research Conference
Sixteen mycobacteriophages were isolated by students at Illinois Wesleyan University in Bloomington IL using a soil enrichment technique and a Mycobacterium smegmatis host. Each student created and archived a high titer lysate of his or her mycobacteriophage, and of these sixteen, two were selected to be sent in for sequencing, Eidsmoe and Morrow. Morrow was found just outside the Morrow Plots at the University of Illinois at Urbana-Champaign in 2014, and was found to be one of 64 members of the A4 subcluster. Its 51,411 base pair genome is comparable to the average A4 genome of 51,395 base pairs. However, …
Expression And Isolation Of The Bche Encoded Protein Of Rhodobacter Capsulatus In Rhodobacter Capsulatus, Jennifer Chlebek, David Bollivar, Faculty Advisor
Expression And Isolation Of The Bche Encoded Protein Of Rhodobacter Capsulatus In Rhodobacter Capsulatus, Jennifer Chlebek, David Bollivar, Faculty Advisor
John Wesley Powell Student Research Conference
Bacteriochlorophyll plays an essential role in the process of photosynthesis in photosynthetic bacteria, but several of the enzymes involved in the synthesis of this tetrapyrrole are yet to be entirely understood. The step in which the ring structure of the tetrapyrrole is formed is catalyzed by the enzyme Mg-protoporphyrin IX monomethyl ester cyclase (MPE-cyclase) which converts the substrate MPE into protochlorophyllide (Pchlide) and incorporates an oxygen atom from water. The gene bchE has been suggested to encode a protein required for MPE-cyclase activity in the photosynthetic bacterium Rhodobacter capsulatus. In order to study the cyclase enzyme, we attempted to isolate …