Life Sciences Commons^™

Cartilage On The Move: Cartilage Lineage Tracing During Tadpole Metamorphosis, Ryan R. Kerney, Alison L. Brittain, Brian K. Hall, Daniel R. Buchholz

Biology Faculty Publications

The reorganization of cranial cartilages during tadpole metamorphosis is a set of complex processes. The fates of larval cartilage-forming cells (chondrocytes) and sources of adult chondrocytes are largely unknown. Individual larval cranial cartilages may either degenerate or remodel, while many adult cartilages appear to form de novo during metamorphosis. Determining the extent to which adult chondrocytes/cartilages are derived from larval chondrocytes during metamorphosis requires new techniques in chondrocyte lineage tracing. We have developed two transgenic systems to label cartilage cells throughout the body with fluorescent proteins. One system strongly labels early tadpole cartilages only. The other system inducibly labels forming …

Toxic Effects Of Copper, Cadmium, And Methoxychlor Shown By Neutral Red Retention Assay In Two Species Of Freshwater Molluscs, Nikolett Molnar, Peter Fong

Biology Faculty Publications

We used neutral red retention assay in lysosomes of digestive gland cells as an indicator for stress effects by the environmental contaminants Cu, Cd, and the pesticide methoxychlor in two freshwater molluscs, the unionid mussel, Elliptio complanata (Lightfoot) and the ramshorn snail Helisoma trivolvis (Say). Mussels and snails were exposed for 7 and 14 days to Cu and Cd each at nominal concentrations of 2.5 μg/L, 5.0 μg/L, and 10.0 μg/L, and to methoxychlor concentrations of 1.0 μg/L, 10.0μg/L, and 100.0 μg/L. Both mussels and snails exposed to Cu showed a significant increase in the percent of destabilized lysosomes compared …

Conserving Our Cultural Heritage: The Role Of Fungi In Biodeterioration, Hanna Szczepanowska, A. R. Cavaliere

Biology Faculty Publications

The objects of cultural heritage are composed of varied materials which can be affected by diverse microbial communities. The study of these complex and heterogeneous assemblies of materials and microorganisms require an inter- and multi-disciplinary approach. Development of a strategy towards prevention, mitigation of biodeterioration and removal of microorganisms, especially fungi begins with the understanding of the materials' fabric, assessment of causes behind the biodeterioration, and the context in which it occurs.

Three aspects of biodeterioration of cultural heritage are discussed: 1) the multitude of bio-agents' on cultural heritage materials, 2) fungal interaction with substrates, and 3) prevention and conservation …