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Full-Text Articles in Life Sciences

Apoptosis Of Dedifferentiated Hepatoma Cells Is Independent Of Nf-Jb Activation In Response To Lps, M. Ryan Reidy, Janette Ellis, Erin A. Schmitz, David M. Kraus, Gary A. Bulla Jan 2007

Apoptosis Of Dedifferentiated Hepatoma Cells Is Independent Of Nf-Jb Activation In Response To Lps, M. Ryan Reidy, Janette Ellis, Erin A. Schmitz, David M. Kraus, Gary A. Bulla

Gary A. Bulla

Dedifferentiated hepatoma cells, in contrast to most other cell types including hepatoma cells, undergo apoptosis when treated with lipopolysaccharide (LPS) plus the protein synthesis inhibitor cycloheximide (CHx). We recently reported that the dedifferentiated hepatoma cells also exhibit a strong and prolonged NF-jB induction phenotype upon exposure to LPS, suggesting that NF-jB signaling may play a pro-survival role, as reported in several other cell systems. To test the role of NF-jB in preventing LPS-mediated apoptosis, we examined the dedifferentiated cell line M38. Results show that antioxidants strongly inhibited LPS + CHx-mediated cell death in the M38 cells, yet only modestly inhibited …


Dissociation Of The Hepatic Phenotype From Hnf4 And Hnf1x Expression, Gary A. Bulla, David M. Kraus Dec 2004

Dissociation Of The Hepatic Phenotype From Hnf4 And Hnf1x Expression, Gary A. Bulla, David M. Kraus

Gary A. Bulla

Dedifferentiated cells have served as tools to understand the molecular consequences of the loss of tissue-specific pathways. Here we report the characterization of one of these cell lines, M29, which lacks the liver-enriched HNF4-HNF1x pathway, in order to determine if this class of variant cell lines could provide additional information regarding requirements for tissue-type expression. We report that although the liver-specific x1-antitrypsin (a1AT) gene remains silent despite reactivation of the HNF4/HNF1x pathway in the M29 cells, the frequency of activation of an integrated x1AT-APRT transgene is increased 1000-fold in response to these transcription factors. The human x1AT locus (introduced via …


Expression Of Gp73, A Resident Golgi Membrane Protein, In Viral And Nonviral Liver Disease, Raleigh D. Kladney, Xiaoyen Cui, Gary A. Bulla, Elizabeth M. Brunt, Claus J. Fimmel Jun 2002

Expression Of Gp73, A Resident Golgi Membrane Protein, In Viral And Nonviral Liver Disease, Raleigh D. Kladney, Xiaoyen Cui, Gary A. Bulla, Elizabeth M. Brunt, Claus J. Fimmel

Gary A. Bulla

GP73 is a novel type II Golgi membrane protein of unknown function that is expressed in the hepatocytes of patients with adult giant-cell hepatitis (Gene 2000;249:53-65). Its expression pattern in human liver disease and the regulation of its expression in hepatocytes have not been systematically studied. The aims of the present study were to compare GP73 protein levels in viral and nonviral human liver disease and in normal livers, to identify its cellular sources, and to study the regulation of its expression in hepatoma cells in vitro. GP73 protein levels were quantitated in explant livers of patients with well-defined disease …