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Erv41p And Erv46p New Components Of Copii Vesicles Involved In Transport Between The Er And Golgi Complex, Stefan Otte, William J. Belden, Matthew Heidtman, Jay Liu, Ole N. Jensen, Charles Barlowe
Erv41p And Erv46p New Components Of Copii Vesicles Involved In Transport Between The Er And Golgi Complex, Stefan Otte, William J. Belden, Matthew Heidtman, Jay Liu, Ole N. Jensen, Charles Barlowe
Dartmouth Scholarship
Proteins contained on purified COPII vesicles were analyzed by matrix-assisted laser desorption ionization mass spectrometry combined with database searching. We identified four known vesicle proteins (Erv14p, Bet1p, Emp24p, and Erv25p) and an additional nine species (Yip3p, Rer1p, Erp1p, Erp2p, Erv29p, Yif1p, Erv41p, Erv46p, and Emp47p) that had not been localized to ER vesicles. Using antibodies, we demonstrate that these proteins are selectively and efficiently packaged into COPII vesicles. Three of the newly identified vesicle proteins (Erv29p, Erv41p, and Erv46p) represent uncharacterized integral membrane proteins that are conserved across species. Erv41p and Erv46p were further characterized. These proteins co- localized to …