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Full-Text Articles in Life Sciences
Fluorescence Measurements Of Phototrophic Sulfur Bacteria For Applications In Water Column Profiling, Simran Gurdasani
Fluorescence Measurements Of Phototrophic Sulfur Bacteria For Applications In Water Column Profiling, Simran Gurdasani
Undergraduate Research Conference
Unlike plants that produce oxygen during photosynthesis, phototrophic sulfur bacteria use sulfide and sunlight to produce carbohydrates and elemental sulfur. These bacteria require a unique aquatic environment to thrive: one that is anoxic (depleted of oxygen) and rich in hydrogen sulfide. Such conditions are found in a number of stratified lakes around the world including several in Northern Indiana. Studying the ecology and geochemical conditions that promote habitable conditions for phototrophic bacteria in lakes provides insight into the Early Earth (thought to be anoxic), ocean anoxic events of the Mesozoic (70-250 million years ago) and modern low oxygen conditions of …
Methods In Extracting Dna: Using Phenol-Chloroform On Formalin Fixed Human Brain Tissue, Stefanie Rigg, Nathaniel Kennedy
Methods In Extracting Dna: Using Phenol-Chloroform On Formalin Fixed Human Brain Tissue, Stefanie Rigg, Nathaniel Kennedy
Undergraduate Research Conference
Genetic markers of diseases can be studied post-mortem with the isolation of DNA from tissues. While protocols exist for DNA extraction from fresh and formalin-fixed-paraffin embedded tissue and formalin fixed hard tissues such as bone, there are no published methods for formalin fixed soft tissue such as brain. Extractions from formalin fixed soft tissues are more difficult due to the higher amount of cellular degradation that results from fixation. The development of efficient DNA extraction protocols for formalin fixed soft tissues will allow post-mortem study of genetic markers and aid in identification of genetic diseases. Existing techniques of phenol-chloroform extraction …