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Full-Text Articles in Life Sciences
Identification Of The Pre-Botzinger Complex Inspiratory Center In Calibrated “Sandwich” Slices From Newborn Mice With Fluorescent Dbx1 Interneurons, Araya Ruangkittisakul, Andrew Kottick, Marina Cristina D. Picardo, Klaus Ballanyi, Christopher A. Del Negro
Identification Of The Pre-Botzinger Complex Inspiratory Center In Calibrated “Sandwich” Slices From Newborn Mice With Fluorescent Dbx1 Interneurons, Araya Ruangkittisakul, Andrew Kottick, Marina Cristina D. Picardo, Klaus Ballanyi, Christopher A. Del Negro
Arts & Sciences Articles
Inspiratory active pre‐Bötzinger complex (preBötC) networks produce the neural rhythm that initiates and controls breathing movements. We previously identified the preBötC in the newborn rat brainstem and established anatomically defined transverse slices in which the preBötC remains active when exposed at one surface. This follow‐up study uses a neonatal mouse model in which the preBötC as well as a genetically defined class of respiratory interneurons can be identified and selectively targeted for physiological recordings. The population of glutamatergic interneurons whose precursors express the transcription factor Dbx1 putatively comprises the core respiratory rhythmogenic circuit. Here, we used intersectional mouse genetics to …
Laser Ablation Of Dbx1 Neurons In The Pre-Botzinger Complex Stops Inspiratory Rhythm And Impairs Output In Neonatal Mice, Xueying Wang, John A. Hayes, Hanbing Song, Andrew Kottick, Nikolas C. Vann, Maria Cristina D. Picardo, Victoria T. Akins, Christopher A. Del Negro, Ann L. Revill, Gregory D. Funk, M. Drew Lamar
Laser Ablation Of Dbx1 Neurons In The Pre-Botzinger Complex Stops Inspiratory Rhythm And Impairs Output In Neonatal Mice, Xueying Wang, John A. Hayes, Hanbing Song, Andrew Kottick, Nikolas C. Vann, Maria Cristina D. Picardo, Victoria T. Akins, Christopher A. Del Negro, Ann L. Revill, Gregory D. Funk, M. Drew Lamar
Arts & Sciences Articles
To understand the neural origins of rhythmic behavior one must characterize the central pattern generator circuit and quantify the population size needed to sustain functionality. Breathing-related interneurons of the brainstem pre-Botzinger complex (preBotC) that putatively comprise the core respiratory rhythm generator in mammals are derived from Dbx1-expressing precursors. Here, we show that selective photonic destruction of Dbx1 preBotC neurons in neonatal mouse slices impairs respiratory rhythm but surprisingly also the magnitude of motor output; respiratory hypoglossal nerve discharge decreased and its frequency steadily diminished until rhythm stopped irreversibly after 85 +/- 20 (mean +/- SEM) cellular ablations, which corresponds to …
Atoh1-Dependent Rhombic Lip Neurons Are Required For Temporal Delay Between Independent Respiratory Oscillators In Embryonic Mice, Srinivasan Tupal, Guang-Yi Ling, Paul A. Gray, Maria Cristina D. Picardo, Christopher A. Del Negro
Atoh1-Dependent Rhombic Lip Neurons Are Required For Temporal Delay Between Independent Respiratory Oscillators In Embryonic Mice, Srinivasan Tupal, Guang-Yi Ling, Paul A. Gray, Maria Cristina D. Picardo, Christopher A. Del Negro
Arts & Sciences Articles
All motor behaviors require precise temporal coordination of different muscle groups. Breathing, for example, involves the sequential activation of numerous muscles hypothesized to be driven by a primary respiratory oscillator, the preBotzinger Complex, and at least one other as-yet unidentified rhythmogenic population. We tested the roles of Atoh1-, Phox2b-, and Dbx1-derived neurons (three groups that have known roles in respiration) in the generation and coordination of respiratory output in embryonic mice. We found that Dbx1-derived neurons are necessary for all respiratory behaviors, whereas independent but coupled respiratory rhythms persist from at least three different motor pools after eliminating or silencing …
Pain Perception And Perspective Taking In Spinal Cord Injury Patients, Caitlin J. Duckett
Pain Perception And Perspective Taking In Spinal Cord Injury Patients, Caitlin J. Duckett
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