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P2x Antagonists Inhibit Styryl Dye Entry Into Hair Cells, M. A. Crumling, M. Tong, K. L. Aschenbach, L. Liu
P2x Antagonists Inhibit Styryl Dye Entry Into Hair Cells, M. A. Crumling, M. Tong, K. L. Aschenbach, L. Liu
University Faculty Publications and Creative Works
The styryl pyridinium dyes, FM1-43 and AM1-43, are fluorescent molecules that can permeate the mechanotransduction channels of hair cells, the sensory receptors of the inner ear. When these dyes are applied to hair cells, they enter the cytoplasm rapidly, resulting in a readily detectable intracellular fluorescence that is often used as a molecular indication of mechanotransduction channel activity. However, such dyes can also permeate the ATP receptor, P2X2. Therefore, we explored the contribution of P2X receptors to the loading of hair cells with AM1-43. The chick inner ear was found to express P2X receptors and to release ATP, similar to …
P2x Antagonists Inhibit Styryl Dye Entry Into Hair Cells, M. A. Crumling, M. Tong, K. L. Aschenbach, L. Liu
P2x Antagonists Inhibit Styryl Dye Entry Into Hair Cells, M. A. Crumling, M. Tong, K. L. Aschenbach, L. Liu
University Faculty Publications and Creative Works
The styryl pyridinium dyes, FM1-43 and AM1-43, are fluorescent molecules that can permeate the mechanotransduction channels of hair cells, the sensory receptors of the inner ear. When these dyes are applied to hair cells, they enter the cytoplasm rapidly, resulting in a readily detectable intracellular fluorescence that is often used as a molecular indication of mechanotransduction channel activity. However, such dyes can also permeate the ATP receptor, P2X2. Therefore, we explored the contribution of P2X receptors to the loading of hair cells with AM1-43. The chick inner ear was found to express P2X receptors and to release ATP, similar to …