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More Fish (1 Of 4), George Mcnamara
More Fish (1 Of 4), George Mcnamara
George McNamara
More Stellaris FRISH and GPU Deconvolution, 20140313Thur - part 1 of 4
Please see other content in the FISH Imaging section of
http://works.bepress.com/gmcnamara/subject_areas.html
for details on the microscope. Briefly: Leica DMI6000 inverted microscope, SOLA lamp, Hamamatsu FLASH4.0 sCMOS camera, 100 nm XY, 200 nm Z pixels, 32 planes (16 um Z), MetaMorph 7.8 acquisition software.
Four zip files:
http://works.bepress.com/gmcnamara/43
http://works.bepress.com/gmcnamara/44
http://works.bepress.com/gmcnamara/45
http://works.bepress.com/gmcnamara/46
with two dataset (001, 002), raw and deconvolved using Bruce & Butte GPU deconvolution in Fiji ImageJ,
http://www.ncbi.nlm.nih.gov/pubmed/23482010
http://www.opticsinfobase.org/vjbo/fulltext.cfm?uri=oe-21-4-4766&id=249375 (free download, as are all Optics Express papers).
http://tcell.stanford.edu/software.html
http://lists.umn.edu/cgi-bin/wa?A2=ind1312&L=confocalmicroscopy&P=5534
Note: "001 raw" (bepress 43) contains my "imaging SOP" …
More Fish (2 Of 4), George Mcnamara
More Fish (2 Of 4), George Mcnamara
George McNamara
More Stellaris FISH and GPU Deconvolution, 20140313Thur - part 2 of 4
Please see other content in the FISH Imaging section of
http://works.bepress.com/gmcnamara/subject_areas.html
for details on the microscope. Briefly: Leica DMI6000 inverted microscope, SOLA lamp, Hamamatsu FLASH4.0 sCMOS camera, 100 nm XY, 200 nm Z pixels, 32 planes (16 um Z), MetaMorph 7.8 acquisition software.
Four zip files:
http://works.bepress.com/gmcnamara/43
http://works.bepress.com/gmcnamara/44
http://works.bepress.com/gmcnamara/45
http://works.bepress.com/gmcnamara/46
with two dataset (001, 002), raw and deconvolved using Bruce & Butte GPU deconvolution in Fiji ImageJ,
http://www.ncbi.nlm.nih.gov/pubmed/23482010
http://www.opticsinfobase.org/vjbo/fulltext.cfm?uri=oe-21-4-4766&id=249375 (free download, as are all Optics Express papers).
http://tcell.stanford.edu/software.html
http://lists.umn.edu/cgi-bin/wa?A2=ind1312&L=confocalmicroscopy&P=5534
Note: "001 raw" (bepress 43) contains my "imaging SOP" …
More Fish (3 Of 4), George Mcnamara
More Fish (3 Of 4), George Mcnamara
George McNamara
More Stellaris FISH and GPU Deconvolution, 20140313Thur - part 3 of 4
Please see other content in the FISH Imaging section of
http://works.bepress.com/gmcnamara/subject_areas.html
for details on the microscope. Briefly: Leica DMI6000 inverted microscope, SOLA lamp, Hamamatsu FLASH4.0 sCMOS camera, 100 nm XY, 200 nm Z pixels, 32 planes (16 um Z), MetaMorph 7.8 acquisition software.
Four zip files:
http://works.bepress.com/gmcnamara/43
http://works.bepress.com/gmcnamara/44
http://works.bepress.com/gmcnamara/45
http://works.bepress.com/gmcnamara/46
with two dataset (001, 002), raw and deconvolved using Bruce & Butte GPU deconvolution in Fiji ImageJ,
http://www.ncbi.nlm.nih.gov/pubmed/23482010
http://www.opticsinfobase.org/vjbo/fulltext.cfm?uri=oe-21-4-4766&id=249375 (free download, as are all Optics Express papers).
http://tcell.stanford.edu/software.html
http://lists.umn.edu/cgi-bin/wa?A2=ind1312&L=confocalmicroscopy&P=5534
Note: "001 raw" (bepress 43) contains my "imaging SOP" …
More Fish (4 Of 4), George Mcnamara
More Fish (4 Of 4), George Mcnamara
George McNamara
More Stellaris FISH and GPU Deconvolution, 20140313Thur - part 4 of 4
Please see other content in the FISH Imaging section of
http://works.bepress.com/gmcnamara/subject_areas.html
for details on the microscope. Briefly: Leica DMI6000 inverted microscope, SOLA lamp, Hamamatsu FLASH4.0 sCMOS camera, 100 nm XY, 200 nm Z pixels, 32 planes (16 um Z), MetaMorph 7.8 acquisition software.
Four zip files:
http://works.bepress.com/gmcnamara/43
http://works.bepress.com/gmcnamara/44
http://works.bepress.com/gmcnamara/45
http://works.bepress.com/gmcnamara/46
with two dataset (001, 002), raw and deconvolved using Bruce & Butte GPU deconvolution in Fiji ImageJ,
http://www.ncbi.nlm.nih.gov/pubmed/23482010
http://www.opticsinfobase.org/vjbo/fulltext.cfm?uri=oe-21-4-4766&id=249375 (free download, as are all Optics Express papers).
http://tcell.stanford.edu/software.html
http://lists.umn.edu/cgi-bin/wa?A2=ind1312&L=confocalmicroscopy&P=5534
Note: "001 raw" (bepress 43) contains my "imaging SOP" …
Time Series Data For 3b Image Processing, George Mcnamara
Time Series Data For 3b Image Processing, George Mcnamara
George McNamara
Time series data for 3B image processing
Four time series data sets of Stellaris single molecules RNA FISH (fluorescence in situ hybridization). All four datasets are 301 imaqe planes. FISH probes are to TOP1 mRNA (Topoisomerase I)in Saos osterosarcoma cells.
One dataset is 10 millisecond exposures (very dim), acquired in Streaming acquisition mode (no hardware overhead).
The other three datasets are a three consecutive planes of the same XY field of view.
I have included image processing results for:
PiMP - a fast method developed by Sebastian Munck et al 2012, http://www.ncbi.nlm.nih.gov/pubmed/?term=22357945
and greatly improved performance by Glen Macdonald (Seattle). …