Life Sciences Commons^™

Stratification Of Potassium And Phosphorus In Tillage Vs. No-Till Systems And Uptake In Corn (Zea Mays) In Western Kentucky, Ava Isaacs, Kyle Krieger, Christopher Powell

Scholars Week

There are 12 nutrients essential for plant growth, two of these are phosphorus and potassium. The demand and need for these nutrients are very high in agricultural systems including corn. Phosphorus stimulates root and shoot growth and is essential for seedling growth and stability. Potassium increases tolerance to water stress, as well as resistance to disease, these are both critical in terms of soil fertility and in determining what fertilizers to apply as well as the amount of fertilizer needed. Both nutrients are not readily available for plant uptake in the soil and are largely lost during harvest, which could …

Attitudes Of South-Central Kentucky Residents Towards Coyotes And Their Management, Andrew T. Mcqueen, Stephen Sumithran Phd

Posters-at-the-Capitol

The existence of the coyote in the eastern U.S. is a recent development and the public’s perception of coyotes has been widely studied in urban areas. However, perceptions in rural areas, especially rural Appalachia, have been largely unstudied. It is necessary for wildlife managers to understand the public’s perceptions of coyotes and coyote management when creating management plans. We conducted a public survey in South-central Kentucky to determine perceptions toward coyotes and coyote management methods. The survey contained questions about demographics (age, gender, county of residence, landownership, etc.), perceptions (fears, opinions), knowledge (true/false questions), and preferred management practices (hunting, trapping, …

Lai As Affected By Flashing-Light Interception With Corn And Soybeans, Josh W. Pohl, Jondavid Mcclanahan, Jake Eaker

Scholars Week

LAI as Affected by Flashing-Light Interception With

Corn (Zea mays) and Soybeans (Glycine max)

Corn (Zea mays) and Soybean (Glycine max) are both staple crops in global agriculture. With growing concern regarding food security and production, indoor cultivation of corn and soybean plants has become a question of interest. This study looks at the application of different lighting regimes for corn and soybeans and effects on development. Light that can be used by plants is called photosynthetically active radiation (PAR). PAR is the solar radiation range of energy that plants use for …

Soil Properties Associated With Land Management Practices In Western Kentucky, Ava Isaacs, Gracey Moffitt

Scholars Week

Soil Properties Associated with Land Management Practices in Western Kentucky

1. A. Isaacs, G. Moffitt, W. Sides, and Corey Hale

Mentor: Dr. I.P. Handayani

Hutson School of Agriculture, Murray State University, Kentucky, USA

Abstract

One of the most important factors regarding soil and plant health is nutrient availability and fertility. Of these two factors that were mentioned available potassium is a key component. With that being said we decided that it would be beneficial to see how potassium can be traced within the soil in various locations after fertilizer is applied. There were two parts to this experiment. Part one was …

Foliar Application Of Acetic Acid And Effectiveness Against Drought At Different Time Points In Soybean (Glycine Max) Development, Miranda Rudolph, Megan Taylor Phd.

Posters-at-the-Capitol

Drought is arguably the most serious environmental factor affecting crop production today. A plant's response to drought stress is extremely complex, affecting numerous metabolic pathways that are all interconnected in their responses. Due to this complex nature, scientists do not fully understand the implications of different treatments and how they affect these processes. Acetic acid has been shown to improve plant growth and resistance to drought stress, but exactly how it accomplishes this is also unknown. Soybeans (Glycine max) are one of the most important crops worldwide and can suffer significant yield loss due to drought stress. As climate change …

Foliar Application Of Acetic Acid And Effectiveness Against Drought At Different Time Points In Soybean Development, Miranda Rudolph

Scholars Week

Abstract

Drought is arguably the most serious environmental factor affecting crop production today. A plants response to drought stress is extremely complex, affecting numerous metabolic pathways that are all interconnected in their responses. Due to this complex nature, scientists do not fully understand the implications of different treatments and how they affect these processes. Acetic acid has been shown to improve plant growth and resistance to drought stress, but exactly how it accomplishes this is also unknown. Soybeans (Glycine max) are one of the most important crops worldwide and can suffer significant yield loss due to drought stress. As climate …

Mapping Fetch And Diel Movements Of Silver Carp Hypophthalmichthys Molitrix Within Kentucky Lake And Lake Barkley With Gis, Levi Umland

Scholars Week

Title: Mapping Silver Carp movements, relative exposure, and fetch within inland reservoirs using Geographic Information Systems.

Author: Levi G Umland

Invasive species across our country have continued to threaten our native species, compete for habitat via interspecific competition, and have caused economic impacts. Silver Carp (Hypophthalmichthys molitrix), commonly referred to as Asian Carp, have remained in the invasive species spotlight throughout the southern and mid-western states as they have continued to spread. Silver Carp have successfully spread through our rivers and reservoirs, but little is known about Silver Carp movement rates/behavior within reservoirs due to their lower abundance within …

Using Bioenergetics To Model The Effects Of Climate Change On Bluntnose Minnows Pimephales Notatus In A Western Kentucky Stream, Christian Slone, Christian Slone

Student Scholarship & Creative Works

Bioenergetics considers a variety of factors like consumption, excretion, and metabolism to quantify the energy use of an organism. A common use of bioenergetics modeling is to help solve fish stock problems. This project looks to develop a bioenergetics model for Bluntnose Minnows Pimephales notatus to help predict effects of climate change on the biodiversity of western Kentucky streams. We used R and NetLogo to create an agent-based bioenergetics model to simulate the size distribution of Bluntnose Minnows under various temperature regimes. Bluntnose Minnow metabolism increases, and consumption decreases with an increase in water temperature. Fish growth seems to be …

Morph- And Sex-Specific Differences In Corticosterone Of The Arizona Tiger Salamander (Ambystoma Mavortium Nebulosum), Megan Zerger

Scholars Week

Life history morph, sex, and body condition are traits that may influence stress within salamander populations because of differences in physiology and environmental conditions. Given widespread declines and the effects chronic stress can have on amphibian health, it is important to understand within-population drivers of stress and how population level variation may influence population viability. Thus, the objective of our study was to assess how corticosterone varies within the Arizona tiger salamander (Ambystoma mavortium nebulosum) population at the Mexican Cut Nature Preserve. We used a non-invasive skin swabbing method to collect baseline and elevated corticosterone from paedomorph (aquatic …

Understanding Relationships Between Stress, Snake Fungal Disease, And Parasitism In Wild Cottonmouth Snake Populations, Emma Fehlker Campbell

Scholars Week

Chronically raised stress levels are known to correlate with decreased immune function in vertebrates, possible leading to increased susceptibility to infections including parasitism and Snake Fungal Disease (SFD). Helminth endoparasites and Hemoparasites are frequently found in wild populations but little is known about their physiological effects. Additionally, parasite infection may have interactive effects with diseases including SFD. To our knowledge few studies have assessed potential interactions between SFD and parasites in snakes. Thus, the objective of our study is to assess the individual and interactive effects of Helminth endoparasites, hemoparasites, and snake fungal disease on cottonmouth physiological stress. We are …

The Impact Of Hemp Cropping Systems On Soil Quality In Western Kentucky, Jack Eaker

Scholars Week

Recently, hemp farming systems are increasingly popular in Kentucky replacing tobacco cropping systems. However, the study on the impacts of hemp cropping on soil properties is rarely conducted, resulting in limited data on soil’s behavior/changes under these management practices. Therefore, the objective of this study was to determine soil characteristics including compaction, acidity level, moisture level, and water retention. The measurements were collected from three different hemp fields, three crop rotation studies ( in 2020 - corn, soybean and tobacco) and a bare field adjacent to these fields. All fields are located at Murray State University West Farm, Calloway County, …

The Nature Of Genes Expressed Differently After Environmental Drug Exposure, Nicci Siffel

Scholars Week

The Nature of Genes Expressed Differently after Environmental Drug Exposure

N. Siffel, S. Anderson, B. Subedi, D.R. Hammond-Weinberger

Drug use, of prescription or illicit varieties, alter bodily functions in different ways, especially in the nervous system. These drugs are found in sources of drinking water because they are not removed by wastewater treatment facilities. To investigate the effects of these drugs as they appear in mixtures, we exposed groups of zebrafish embryos to cocktails of drugs and screened for genes that were differentially expressed between the experimental and control groups and could thus give more insight into their biological functions. …

Evaluating The Impact Of Common Fertilizers On Soil Properties After Planting Tomatoes And Marigolds, Rachel Stuckey, Iin Handayani

Scholars Week

There are many name brand fertilizers that are used today in gardening in the state of Kentucky. Due to the standardization of name brand fertilizer production in factories, this can cause the fertilizers to compromise the nutrients of soil, while all-natural fertilizers are seen as better quality for the consumers, plants and soils. Alaska, Miracle Grow, Monty’s, Pure Gold, Tiger Bloom are the top five fertilizers sold in the state of Kentucky. This study aims to determine the impact of five top commercial fertilizers on soil quality indicators including organic matter, acidity level and moisture content. These fertilizers were applied …

Germination And Early Seedling Growth Of Vegetables Under Various Salinity Stresses, Waqas Ahmad, Muhammad Haider Ali, Iin P. Handayani Dr., Brian Parr Dr.

Scholars Week

Vegetables are an important part of our daily diet. As salinity levels in soil and irrigation water have increased, biomass production of horticultural crops has been reduced during the past few years. The main objective of this study was to check the effect of different salinity levels (0mM, 25mM, 50mM, 100mM, 200mM NaCl) on the germination percentage and the growth rate of young seedlings of vegetables (tomato, turnip, onion, fenugreek and cauliflower) comprehensively. This experiment was designed through Factorial under Completely Randomize Design (CRD) and the results were analyzed through all Pair-wise comparison of means using Tukey test (P ≤ …

The Effects Of Erosion Structures On Triplett Creek From The Fall Of 2016, 2017, And 2018, Haylee Winters

Posters-at-the-Capitol

Triplett Creek is a tributary of the Licking River. It runs through Morehead, KY, where we are conducting our research.

The creek was modified in the 1970s but construction was halted before the project was complete. Unfortunately, the result was an increase in overflow and subsequent riverbank erosion. The city installed sediment trap rocks in hopes of slowing down the water and decreasing erosion. We are using drones and photogrammetric software to map the area and compare images of the river to determine erosion rates. The new images will be compared to ones taken in previous years to visualize the …

Prenylation In The Moss Physcomitrella Patens, Noela Botaka, Susana Perez-Martinez, Liang Bao, Parul Singh, Mark Running

Posters-at-the-Capitol

Protein prenylation is a post-translational modification that involves the addition of lipid groups to the end of a target protein and is necessary for protein activity. Prenylation has important roles in the cell: targeting and localizing proteins to subcellular compartments and promoting protein-protein interactions. Recently, we have found Protein Prenyltransferase Alpha Subunit-like (PPAL), which shares structural similarities to known prenylation enzymes. However, the biochemical function of PPAL is still unknown. PPAL is present in a single copy in other plants examined to date but is present in two copies in moss. Knockouts in our lab of either PpPAL1 or PpPAL2 …

A Crispr Platform For Rapid And Inducible Genome Editing In Human Non-Small Cell Lung Cancer Cells, Lloyd Bartley

Posters-at-the-Capitol

Non-small cell lung cancer (NSCLC) accounts for about 85% of lung cancer, which is the leading cause of cancer death in the world. High mortality rate associated with NSCLC is partially attributed to the limited understanding of NSCLC as well as ineffective therapeutic treatments. The initiation and progression of NSCLC involves genetic changes leading to alterations in the control of tissue development and homeostatic maintenance. Better knowledge about these genetic abnormalities is imperative for developing new chemotherapeutic drugs for NSCLC. Recent research demonstrates that the expression of paraoxonase 2 (PON2), a lactonase/arylesterase with anti-oxidant properties, are markedly enhanced in cancer …

Soil Responses To One-Season Winter Cover Crops, Mckenna Morgan, Iin Handayani

Posters-at-the-Capitol

Soil Responses to One-Season Winter Cover Crops

McKenna Morgan and I. P. Handayani

Hutson School of Agriculture Murray State University, KY, USA

Abstract

In regions like western Kentucky where wooded lots are common there is a growing interest in how to utilize them so that land profits can be maximized. The frequently poor soils in wooded areas due to erosion and lack of ground cover vegetation often cause complications in the creation of pasture areas. Therefore, the objective of this study was to assess the effect of growing winter cover crops on soils to prepare for pasture systems in the …

Consumption Profiles Of Illicit And Neuropsychiatric Drugs In Urban And Rural Communities In Kentucky Using Sewage Epidemiology, Tara Croft, Katelyn Foppe, Rhiannon Huffines, Bikram Subedi

Posters-at-the-Capitol

The drug overdose deaths in the USA increased by ~20% from 2015 to 2016 to the total >63,600 drug overdose deaths in 2016. Kentucky is among the highest rate of drug overdose death states in the USA. The current estimates of the prevalence of substance abuse are based on the self-reported surveys, overdose/toxicological reports, and drug-related crime statistics. Survey-based conventional approaches are not only cost and time-intensive but also underestimate the actual consumption of drugs. As “wastewater never lie,” the drug residues in raw wastewater collected from the centralized wastewater treatment plants were utilized to determine the consumption rate of …

Impact Of Aerobic Compost Tea On Growth Of Lactuca Sativa Within A Peat-Based Growing Media, Ella Potts, Jessica Stoner, Alyx Shultz, Iin Handayani

Posters-at-the-Capitol

Compost and compost tea have been shown in practice to be valuable soil amendments across many horticultural applications; however, little rigorous quantitative work has been completed. Researchers designed a side-by-side trial with two replications to quantifiably study the impact of compost tea on the growth and vigor of soil-grown lettuce. A recycled poultry-litter-based aerobic compost tea was used for the treatment. Six lactuca sativa plants received each treatment or control. Researchers hypothesized the treatment plants would show more vigor and vegetative growth than control. The null hypothesis was treatment plants would be identical to control lettuce. Researchers failed to reject …

Development Of A Pd-L1 Pet Imaging Biomarker, Caleb Jack Bridgwater

Posters-at-the-Capitol

Immunotherapy strategies are very promising treatments for cancer patients. Specifically, Immune checkpoint inhibitor therapy focusing on the PD-1/PD-L1 pathway shows long-lasting positive results in many cancer patients. Unfortunately, not all the patients can benefit from this highly effective treatment. Hence, there is a great need for predictive biomarkers. Immunohistochemical (IHC) staining has been used as a way of predicting patient response, yet shows many problems. For example, IHC utilizes an invasive biopsy and sample fixing, which creates an incomplete and delayed picture of the patient’s biochemistry and the tumor microenvironment, consequently ignoring metastases.

The purpose of this study is to …

Histone Deacetylation Is The Primary Epigenetic Mechanism For Silencing Of Tumor Suppressor Gene - Tissue Factor Pathway Inhibitor-2 In Hepatocellular Carcinoma Cells, Alisha Deshmukh, Shirish Barve

Posters-at-the-Capitol

Hepatocellular carcinoma (HCC) is the third leading cause of cancer related mortality worldwide. With a survival rate of less than 5 percent, a therapeutic treatment is desperately needed to manage this disease. Many epigenetic mechanisms that underlay HCC are being identified. A frequently silenced pathway tissue factor pathway inhibitor-2 (TFPI-2) is a critical tumor suppressor gene. In HCC, inactivation of TFPI-2 leads to tumor growth. Recent research indicated Fas L plays a major role in apoptosis as part of HCC. For the purposes of this study, the phytochemical Curcumin was explored to observe its possible effects on the epigenetic mechanisms …

Neuronal Degeneration And Short-Term Memory Impairment After Tbi, Obiamaraije Igwe

Posters-at-the-Capitol

Traumatic brain injury (TBI) was associated with impaired short-term memory with causes of vehicle accidents and falls. Protein plaques containing fibrinogen (Fg), are associated with memory loss. After TBI, Fg in blood was higher than normal (>~2 mg/ml), which resulted in increased Fg in extravascular space. Therefore, Fg bonded to its endothelial receptor intercellular adhesion molecule-1 (ICAM-1). Fg then interacted with cellular prion protein (PrPC), which had a strong effect on the loss of memory and cognition. Mechanisms of Fg and PrPC complex formation and its functional implication are not known. This present study tested the level of Fg-PrPC …

Swimming Mechanisms Of Temperate Forest Ants, Noah D. Gripshover, Evan M. Gora, Stephen P. Yanoviak

Posters-at-the-Capitol

Swimming Mechanisms of Temperate Forest Ants (Camponotus pennsylvanicus and Formica subsericea)

Noah D. Gripshover, Evan M. Gora, and Stephen P. Yanoviak

University of Louisville

Abstract

Environmental challenges shape the evolution of animal behavior and morphology. For wingless terrestrial invertebrates like ants, pools of water on the forest floor are particularly dangerous. Here we show that ants can overcome this obstacle using a modified gait to transverse the water surface. We compared the locomotor morphology and swimming performance of two arboreal ant species that are common in Kentucky (Camponotus pennsylvanicus and Formica subsericea). We defined performance as speed …

Kentucky Lake: An Opportunity For Long Term Monitoring Of Fish Communities, Bradley Hartman

Scholars Week

Global aquatic ecosystems are impacted by a variety of mechanisms including habitat destruction, fish overharvest, and the introduction of invasive species. Long term monitoring of an ecosystem’s fish community, along with abiotic and biotic factors that influence the fish community, is crucial in establishing effective management strategies. Currently, numerous monitoring programs exist across the United States. However, a long term monitoring program of non-game and non-commercial fish community dynamics has not been established within Kentucky Lake in Western Kentucky. Preliminary sampling of the Kentucky Lake fish community was performed using boat electroshocking and surface trawling. Four embayments within Kentucky Lake …

The Effects Of The Fungus Beauvaria Sp. On The Cave Cricket, Hadenoecus Subterraneus, Christina N. Walker, Derrick J. Jent, Claire A. Fuller

Scholars Week

The cave cricket, Hadenoecus subterraneus, is a keystone species in cave ecosystems within Mammoth Cave National Park (MCNP). Within MCNP, many cricket cadavers have been found

with a thick, white fungus growing on them; this fungus has previously been identified to be Beauveria bassiana. However, new molecular data suggests that this may actually be the species B. amorpha. Cricket cadavers with Beauveria sp. were collected from MCNP and cultured on potato dextrose agar. Cultures will be sent to the USDA for a genetic analysis and identification of the fungus. The purpose of this study is to examine if the relationship …