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A Beta-Linked Mannan Inhibits Adherence Of Pseudomonas Aeruginosa To Human Lung Epithelial Cells, Ali Azghani, Izola Williams, David B. Holiday, Alice R. Johnson
A Beta-Linked Mannan Inhibits Adherence Of Pseudomonas Aeruginosa To Human Lung Epithelial Cells, Ali Azghani, Izola Williams, David B. Holiday, Alice R. Johnson
Ali Azghani
Adherence through carbohydrate-binding adhesins is an early step in colonization of the lung by gram-negative organisms, and because published data indicate that binding involves mannose groups, we tested the ability of a β-linked acetylmannan (acemannan) to inhibit adherence of Pseudomonus aeruginosa to cultures of human lung epithelial cells. Adherence of radiolabelled P.aeruginosa to A549 cells (a type II-like pneurnocyte line) increased linearly with the duration of the incubation. Acemannan inhibited adherence of bacteria, and the extent of inhibition was related to the concentration of the mannan. Inhibition required continued contact between acemannan and the target epithelial cells; cells washed free …