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Mark R Wilson

2013

Articles 1 - 4 of 4

Full-Text Articles in Life Sciences

Structural Characterization Of Clusterin-Chaperone Client Protein Complexes, Amy Wyatt, Justin Yerbury, Mark Wilson May 2013

Structural Characterization Of Clusterin-Chaperone Client Protein Complexes, Amy Wyatt, Justin Yerbury, Mark Wilson

Mark R Wilson

Clusterin (CLU) is a potent extracellular chaperone that inhibits protein aggregation and precipitation otherwise caused by physical or chemical stresses (e.g. heat, reduction). This action involves CLU forming soluble high molecular weight (HMW) complexes with the client protein. Other than their unquantified large size, the physical characteristics of these complexes were previously unknown. In this study, HMW CLU-citrate synthase (CS), HMW CLU-fibrinogen (FGN), and HMW CLU-glutathione S-transferase (GST) complexes were generated in vitro, and their structures studied using size exclusion chromatography (SEC), ELISA, SDS-PAGE, dynamic light scattering (DLS), bisANS fluorescence, and circular dichroism spectrophotometry (CD). Densitometry of …

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Extracellular Chaperones Prevent A42-Induced Toxicity In Rat Brains, R Cascella, S Conti, F Tatini, E Evangelisti, T Scartabelli, F Casamenti, Mark Wilson, F Chiti, C Cecchi Dec 2012

Extracellular Chaperones Prevent A42-Induced Toxicity In Rat Brains, R Cascella, S Conti, F Tatini, E Evangelisti, T Scartabelli, F Casamenti, Mark Wilson, F Chiti, C Cecchi

Mark R Wilson

No abstract provided.

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Protease-Activated Alpha-2-Macroglobulin Can Inhibit Amyloid Formation Via Two Distinct Mechanisms, A Wyatt, P Constantinescu, H Ecroyd, C Dobson, Mark Wilson, J Kumita, J Yerbury Dec 2012

Protease-Activated Alpha-2-Macroglobulin Can Inhibit Amyloid Formation Via Two Distinct Mechanisms, A Wyatt, P Constantinescu, H Ecroyd, C Dobson, Mark Wilson, J Kumita, J Yerbury

Mark R Wilson

No abstract provided.

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Extracellular Chaperones And Proteostasis, A Wyatt, J Yerbury, H Ecroyd, Mark Wilson Dec 2012

Extracellular Chaperones And Proteostasis, A Wyatt, J Yerbury, H Ecroyd, Mark Wilson

Mark R Wilson

No abstract provided.

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