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Articles 1 - 5 of 5
Full-Text Articles in Life Sciences
A Phylogeny Of Belonolaimus Populations In Florida Inferred From Dna Sequences, Byron Adams, U. Gozel, K. Nguyen, R. Inserra, R. Giblin-Davis
A Phylogeny Of Belonolaimus Populations In Florida Inferred From Dna Sequences, Byron Adams, U. Gozel, K. Nguyen, R. Inserra, R. Giblin-Davis
Byron Adams
The D2-D3 and ITS regions of rDNA from 33 Florida populations of Belonolaimus spp. were sequenced and subjected to phylogenetic analysis. Our objective was to derive a theoretical evolutionary framework for interpreting phenotypic differences as they relate to the taxonomy of the genus. The most striking aspect of the phylogenetic analysis is that none of the three nominal species (B. longicaudatus, B. euthychilus, and B. gracilis) are monophyletic. Additionally, two taxa appear to have discordant ITS and LSU sequences. Three major clades of B. longicaudatus exhibited discernible, overlapping, geographic foci from east to west across the peninsula. Morphological character states …
Transcriptomic Analysis Of The Entomopathogenic Nematode Heterorhabditis Bacteriophora Tto1, Byron Adams, Xiaodong Bai, Todd Ciche, Sandra Clifton, Randy Gaugler, Parwinder Grewal, Saskia Hogenhout, John Spieth, Paul Sternberg, Richard Wilson
Transcriptomic Analysis Of The Entomopathogenic Nematode Heterorhabditis Bacteriophora Tto1, Byron Adams, Xiaodong Bai, Todd Ciche, Sandra Clifton, Randy Gaugler, Parwinder Grewal, Saskia Hogenhout, John Spieth, Paul Sternberg, Richard Wilson
Byron Adams
Background: The entomopathogenic nematode Heterorhabditis bacteriophora and its symbiotic bacterium, Photorhabdus luminescens, are important biological control agents of insect pests. This nematode-bacterium-insect association represents an emerging tripartite model for research on mutualistic and parasitic symbioses. Elucidation of mechanisms underlying these biological processes may serve as a foundation for improving the biological control potential of the nematode-bacterium complex. This large-scale expressed sequence tag (EST) analysis effort enables gene discovery and development of microsatellite markers. These ESTs will also aid in the annotation of the upcoming complete genome sequence of H. bacteriophora. Results: A total of 31,485 high quality ESTs were generated …
Decline In A Dominant Invertebrate Species Contributes To Altered Carbon Cycling In A Low-Diversity Soil Ecosystem, Byron Adams, J. Barrett, Ross Virginia, Diana Wall
Decline In A Dominant Invertebrate Species Contributes To Altered Carbon Cycling In A Low-Diversity Soil Ecosystem, Byron Adams, J. Barrett, Ross Virginia, Diana Wall
Byron Adams
Low-diversity ecosystems cover large portions of the Earth's land surface, yet studies of climate change on ecosystem functioning typically focus on temperate ecosystems, where diversity is high and the effects of individual species on ecosystem functioning are difficult to determine. We show that a climate-induced decline of an invertebrate species in a low-diversity ecosystem could contribute to significant changes in carbon © cycling. Recent climate variability in the McMurdo Dry Valleys of Antarctica is associated with changes in hydrology, biological productivity, and community composition of terrestrial and aquatic ecosystems. One of the greatest changes documented in the dry valleys is …
Desiccation Survival In An Antarctic Nematode: Molecular Analysis Using Expressed Sequenced Tags, Byron Adams, Bishwo Adhikari, Diana Wall
Desiccation Survival In An Antarctic Nematode: Molecular Analysis Using Expressed Sequenced Tags, Byron Adams, Bishwo Adhikari, Diana Wall
Byron Adams
Background: Nematodes are the dominant soil animals in Antarctic Dry Valleys and are capable of surviving desiccation and freezing in an anhydrobiotic state. Genes induced by desiccation stress have been successfully enumerated in nematodes; however we have little knowledge of gene regulation by Antarctic nematodes which can survive multiple environmental stresses. To address this problem we investigated the genetic responses of a nematode species, Plectus murrayi, that is capable of tolerating Antarctic environmental extremes, in particular desiccation and freezing. In this study, we provide the first insight into the desiccation induced transcriptome of an Antarctic nematode through cDNA library construction …
Environmental Dna Sequencing Primers For Eutardigrades And Bdelloid Rotifers, Byron Adams, Jeremy Whiting, Elizabeth Costello, Kristen Freeman, Andrew Martin, Michael Robeson, Steve Schmidt
Environmental Dna Sequencing Primers For Eutardigrades And Bdelloid Rotifers, Byron Adams, Jeremy Whiting, Elizabeth Costello, Kristen Freeman, Andrew Martin, Michael Robeson, Steve Schmidt
Byron Adams
Background: The time it takes to isolate individuals from environmental samples and then extract DNA from each individual is one of the problems with generating molecular data from meiofauna such as eutardigrades and bdelloid rotifers. The lack of consistent morphological information and the extreme abundance of these classes makes morphological identification of rare, or even common cryptic taxa a large and unwieldy task. This limits the ability to perform large-scale surveys of the diversity of these organisms. Here we demonstrate a culture-independent molecular survey approach that enables the generation of large amounts of eutardigrade and bdelloid rotifer sequence data directly …