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Articles 1 - 5 of 5
Full-Text Articles in Life Sciences
Delineation Of Precursors In Murine Spleen That Develop In Contact With Splenic Endothelium To Give Novel Dendritic-Like Cells., Jonathan Tan, Pravin Periasamy, Helen O'Neill
Delineation Of Precursors In Murine Spleen That Develop In Contact With Splenic Endothelium To Give Novel Dendritic-Like Cells., Jonathan Tan, Pravin Periasamy, Helen O'Neill
Jonathan Tan
Hematopoietic cell lineages are best described in terms of distinct progenitors with limited differentiative capacity. To distinguish cell lineages, it is necessary to define progenitors and induce their differentiation in vitro. We previously reported in vitro development of immature dendritic-like cells (DCs) in long-term cultures (LTCs) of murine spleen, and in cocultures of spleen or bone marrow (BM) over splenic endothelial cell lines derived from LTCs. Cells produced are phenotypically distinct CD11b(hi)CD11c(lo)CD8(-)MHC-II(-) cells, tentatively named L-DCs. Here we delineate L-DC progenitors as different from known DC progenitors in BM and DC precursors in spleen. The progenitor is contained within the …
Haematopoietic Stem Cells In Spleen Have Distinct Differentiative Potential For Antigen Presenting Cells., Jonathan Tan, Helen O'Neill
Haematopoietic Stem Cells In Spleen Have Distinct Differentiative Potential For Antigen Presenting Cells., Jonathan Tan, Helen O'Neill
Jonathan Tan
Dendritic cells (DC) are known to develop from macrophage dendritic progenitors (MDP) in bone marrow (BM), which give rise to conventional (c)DC and monocytes, both dominant antigen presenting cell (APC) subsets in spleen. This laboratory has however defined a distinct dendritic-like cell subset in spleen (L-DC), which can also be derived in long-term cultures of spleen. In line with the restricted in vitro development of only L-DC in these stromal cultures, we questioned whether self-renewing HSC or progenitors exist in spleen with restricted differentiative capacity for only L-DC. Neonatal spleen and BM were compared for their ability to reconstitute mice …
Basking Behavior Of Emydid Turtles (Chysemys Picta Marginata, Graptemys Geographica, And Trachemys Scripta Elegans) In An Urban Landscape, W. Peterman, Travis Ryan
Basking Behavior Of Emydid Turtles (Chysemys Picta Marginata, Graptemys Geographica, And Trachemys Scripta Elegans) In An Urban Landscape, W. Peterman, Travis Ryan
Travis J. Ryan
Basking is common in emydid turtles and is generally accepted to be in thermoregulatory behavior. In 2004, we quantified and described the basking behavior of turtles in the Central Canal of Indianapolis. This canal system runs through an urban landscape that is dominated by fragmented woodlots, residential areas. and commercial areas. We observed that basking turtles exhibited variable basking behavior. with spatial and temporal shins in basking behavior from east-facing banks in the morning to west-facing banks in the afternoon. Turtles in the Central Canal are subject to frequent disturbance, which altered basking behavior. Many turtles forewent aerial basking on …
A Rapid Direct Solvent Extraction Method For The Extraction Of 2-Dodecylcyclobutanone From Irradiated Ground Beef Patties Using Acetonitrile., Amit Kumar
Amit Kumar, DVM, MS, PhD
The amount of irradiated beef in the U.S. market is growing, and a reliable, rapid method is needed to detect irradiated beef and quantify the irradiation dose. The official analytical method (BS EN 1785 2003) that has been adopted by the European Union is time consuming. The objective of this study was to develop a rapid method for the analysis of 2-dodecylcyclobutanone (2-DCB) in irradiated beef. A 5 g sample of commercially irradiated ground beef patty (90/10) was extracted with n-hexane using a Soxhlet apparatus or with acetonitrile via direct solvent extraction. The Soxhlet hexane extract was evaporated to dryness, …
Architecture Of The Potyviruses, Michele Mcdonald, Amy Kendall, Wen Bian, Ian Mccullough, Elizabeth Lio, Wendy Havens, Said Ghabrial, Gerald Stubbs
Architecture Of The Potyviruses, Michele Mcdonald, Amy Kendall, Wen Bian, Ian Mccullough, Elizabeth Lio, Wendy Havens, Said Ghabrial, Gerald Stubbs
Ian McCullough
X-ray fiber diffraction data were obtained and helical pitch and symmetry were determined for seven members of the family Potyviridae, including representatives from the genera Potyvirus, Rymovirus, and Tritimovirus. The diffraction patterns are similar, as expected. There are, however, significant variations in the symmetries, as previously found among the flexible potexviruses, but not among the rigid tobamoviruses. Wheat streak mosaic virus, the only member of the genus Tritimovirus examined, displayed the largest deviations in diffraction data and helical parameters from the other viruses in the group.