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Full-Text Articles in Life Sciences
Self-Assembling Short Oligopeptides And The Promotion Of Angiogenesis, Alisha Sarang-Sieminski, Daria Narmoneva, Olumuyiwa Oni, Shugang Zhang, Jonathan Gertler, Roger Kamm, Richard Lee
Self-Assembling Short Oligopeptides And The Promotion Of Angiogenesis, Alisha Sarang-Sieminski, Daria Narmoneva, Olumuyiwa Oni, Shugang Zhang, Jonathan Gertler, Roger Kamm, Richard Lee
Alisha L. Sarang-Sieminski
Because an adequate blood supply to and within tissues is an essential factor for successful tissue regeneration, promoting a functional microvasculature is a crucial factor for biomaterials. In this study, we demonstrate that short self-assembling peptides form scaffolds that provide an angiogenic environment promoting long-term cell survival and capillary-like network formation in three-dimensional cultures of human microvascular endothelial cells. Our data show that, in contrast to collagen type I, the peptide scaffold inhibits endothelial cell apoptosis in the absence of added angiogenic factors, accompanied by enhanced gene expression of the angiogenic factor VEGF. In addition, our results suggest that the …
Salmon Fibrin Supports An Increased Number Of Sprouts And Decreased Degradation While Maintaining Sprout Length Relative To Human Fibrin In An In Vitro Angiogenesis Model, Alisha Sarang-Sieminski, Keith Gooch
Salmon Fibrin Supports An Increased Number Of Sprouts And Decreased Degradation While Maintaining Sprout Length Relative To Human Fibrin In An In Vitro Angiogenesis Model, Alisha Sarang-Sieminski, Keith Gooch
Alisha L. Sarang-Sieminski
Salmon-derived fibrin has been proposed as a preferred alternative to human or bovine fibrin because of its reduced potential for disease transmission. Here we evaluate salmon fibrin as an alternative ECM support for therapeutic angiogenesis applications, such as vascularizing engineered tissues. Human umbilical vein endothelial cells (HUVEC) seeded on gelatin beads and suspended in either salmon or human fibrin sprouted and formed capillary-like structures. Sprout length was generally increased with the addition of bFGF and VEGF and further increased with the addition of phorbol myristate acetate (PMA). The number of sprouts per bead was increased 61-188% in salmon fibrin relative …
Migration Of Tumor Cells In 3d Matrices Is Governed By Matrix Stiffness Along With Cell-Matrix Adhesion And Proteolysis, Alisha Sarang-Sieminski, Muhammad Zaman, Linda Trapani, Drew Mackellar, Haiyan Gong, Roger Kamm, Alan Wells, Douglas Lauffenburger, Paul Matsudaira
Migration Of Tumor Cells In 3d Matrices Is Governed By Matrix Stiffness Along With Cell-Matrix Adhesion And Proteolysis, Alisha Sarang-Sieminski, Muhammad Zaman, Linda Trapani, Drew Mackellar, Haiyan Gong, Roger Kamm, Alan Wells, Douglas Lauffenburger, Paul Matsudaira
Alisha L. Sarang-Sieminski
Cell migration on 2D surfaces is governed by a balance between counteracting tractile and adhesion forces. Although biochemical factors such as adhesion receptor and ligand concentration and binding, signaling through cell adhesion complexes, and cytoskeletal structure assembly/disassembly have been studied in detail in a 2D context, the critical biochemical and biophysical parameters that affect cell migration in 3D matrices have not been quantitatively investigated. We demonstrate that, in addition to adhesion and tractile forces, matrix stiffness is a key factor that influences cell movement in 3D. Cell migration assays in which Matrigel density, fibronectin concentration, and β1 integrin binding are …