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Growth Of Eimeria Alabamensis From Cattle In Vitro And The Changes In Fine Structure Associated With Schizogony, J. Robert Sampson
Growth Of Eimeria Alabamensis From Cattle In Vitro And The Changes In Fine Structure Associated With Schizogony, J. Robert Sampson
All Graduate Theses and Dissertations, Spring 1920 to Summer 2023
Monolayer primary cultures of cells from bovine embryonic intestine (BEint), kidney (BEK), spleen (BES), and thyroid (BET) and cell line cultures of embryonic bovine trachea ( EBTr) and synovium (BESy)as well as established cell line cultures of Madin-Darby bovine kidney (MDBK), human intestine (Int 407), and Syrian hamster kidney (BHK-21) were inoculated with freshly excysted sporozoites of Eimeria alabamensis and observed for 4 days. Sporozoites penetrated all cell types during the first 24 hours in culture. Numerous intracellular sporozoites, trophozoites, and binucleate schizonts were seen in all cell cultures except Int 407. The best development occurred in BES and MDBK …