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Articles 1 - 6 of 6
Full-Text Articles in Life Sciences
Development Of A Reliable Method To Express And Purify Vaccinia Virus Transcription Factors A7 And D6 In Bacteria, Younghoon Park
Development Of A Reliable Method To Express And Purify Vaccinia Virus Transcription Factors A7 And D6 In Bacteria, Younghoon Park
Seton Hall University Dissertations and Theses (ETDs)
Poxvirus early genes code for viral products involved in host interaction, immune evasion, replication, and transcription of the viral genome. These viral early genes contain a unique and conserved promoter sequence that is recognized by early transcription factors (ETFs). The working hypothesis is pharmacologically targeting the structure of the poxvirus early promoters can inhibit transcription of poxvirus early genes. Our group has identified unique secondary structure which deviates from a scrambled control near the Vaccinia Virus (VACV) E9L promoter. Targeting these unique structures, which most likely may be G-quadruplexes, with structure specific ligands may distort local DNA structures at the …
Mutational Analysis Of The Molluscum Contagiosum Virus Mc160 Protein, Henri Estanbouli
Mutational Analysis Of The Molluscum Contagiosum Virus Mc160 Protein, Henri Estanbouli
Seton Hall University Dissertations and Theses (ETDs)
The Molluscum Contagiosum Virus (MCV), belongs to a family of large DNA viruses called Poxviridae. MCV causes a common skin infection resulting in benign neoplasms. 122 million cases of MC were reported in 2010. The virus is not primarily lethal, though fatalities have occurred due to secondary bacterial infections. Even with the high volume of reported cases, MCV is very much understudied, and its pathogenicity is not well understood. Thus, with that in mind, a more in-depth understanding of the MCV-host interactions is a priority. Presumably during infection, MCV utilizes immune evasion molecules to inhibit activation of the host pro-inflammatory …
Development Of A Novel Method To Express And Purify Vaccinia Virus Early Transcription Factors A7 And D6 Using Bacteria, Omkar M. Gandbhir
Development Of A Novel Method To Express And Purify Vaccinia Virus Early Transcription Factors A7 And D6 Using Bacteria, Omkar M. Gandbhir
Seton Hall University Dissertations and Theses (ETDs)
The eradication of Smallpox is one of the greatest human achievements. However other poxviruses still exist infecting humans (Molluscum Contagiosum, monkeypox, and Vaccinia) or livestock (cowpox, sheeppox). Though vaccines and anti-virals exist, the side effects are serious, and viral resistance has been detected. The genome amongst poxviruses is highly conserved, early gene promoters maintain a specific critical consensus region that can be targeted for development of novel broad spectrum therapeutics. Using Vaccinia early transcription factors (VETFs) a novel method of expressing and purifying VETF A7 and D6 was developed. A7 and D6 are required for transcription of early genes recruiting …
Characterization Of A Mitochondrial Localizing Molluscum Contagiosum Virus Protein: Mc163r, Jesse Coutu
Characterization Of A Mitochondrial Localizing Molluscum Contagiosum Virus Protein: Mc163r, Jesse Coutu
Seton Hall University Dissertations and Theses (ETDs)
Molluscum Contagiosum Virus (MCV) is an obligate human, tumorigenic poxvirus which causes benign skin neoplasms. Reduced inflammation during an MCV infection has been attributed to production of MCV immune evasion molecules (IEMs). IEMs antagonize host immune responses allowing MCV to evade the host immune system and reprogram the host cell for viral growth. MCV IEMs alter apoptosis (MC159, MC160), deregulate the cell cycle targeting retinoblastoma (MC007), and produce a chemokine homolog (MC148). Bioinformatics analysis identified MC163, as another potential host-interacting protein. Further analysis of MC163 resulted in the identification of a mitochondrial localization sequence, putative superoxide dismutase (SOD)-like Cu2+ …
Modified Green Tea Polyphenol, Palmitoyl-Egcg And Black Tea Theaflavins: Promising Natural Herpes Simplex Virus 1 And 2 Agents, Aline Moraes De Oliveira
Modified Green Tea Polyphenol, Palmitoyl-Egcg And Black Tea Theaflavins: Promising Natural Herpes Simplex Virus 1 And 2 Agents, Aline Moraes De Oliveira
Seton Hall University Dissertations and Theses (ETDs)
Green tea and black tea originate from the leaves of Camellia Sinensis plant and contain antioxidants known to possess antiviral properties, including anti-HSV (Herpes Simplex Virus). There is no cure for the diseases caused by HSV infections. While there is a rising concern over the increase in resistant HSV strains, new effective agents for prevention are in critical need to be developed. Green tea’s polyphenol epigallocatechin gallate (EGCG), and its modification palmitoyl-EGCG (p-EGCG), along with black tea theaflavins TF1, TF2, TF3 have been used to study their antiviral effect against HSV type 1 (HSV-1) and HSV type 2 (HSV-2). These …
Functional Analysis Of The Molluscum Contagiosum Virus Mc160 Death Effector Domain-Containing Protein Rxdl Motif, Sarah Weber
Functional Analysis Of The Molluscum Contagiosum Virus Mc160 Death Effector Domain-Containing Protein Rxdl Motif, Sarah Weber
Seton Hall University Dissertations and Theses (ETDs)
The Molluscum contagiosum virus (MCV) is a member of the Poxviridae family that causes benign skin lesions. MCV lesions persist on average for 8-12 months in otherwise healthy individuals. MCV lesions are characterized by reduced inflammation. The persistence and reduction of inflammation at the site of MCV lesions have been attributed to MCV immune evasion genes. MCV encodes two death effector domain (DED) containing proteins, MC159 and MC160. DEDs are found in cellular proteins such as FADD and procaspase-8. These cellular proteins are involved in several innate immune responses such as apoptosis and activation of interferon (IFN). Presumably, MC159 and …