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Full-Text Articles in Life Sciences
Spatial Localization Of Markers And 3d-Cell Model For Study Of The Human Cytomegalovirus Cytoplasmic Virion Assembly Compartment, Taylor Alan Vensko
Spatial Localization Of Markers And 3d-Cell Model For Study Of The Human Cytomegalovirus Cytoplasmic Virion Assembly Compartment, Taylor Alan Vensko
Wayne State University Theses
Human cytomegalovirus (HCMV) causes severe disease in immunocompromised individuals and is a leading cause of congenital disease. Efficient assembly of virions (viral particles) is an intricate process that requires modulation and modification of host systems. HCMV induces an extensive rearrangement of the cellular endocytic recycling compartment into the site of virion maturation and egress: the cytoplasmic virion assembly compartment (cVAC). The HCMV cVAC is a distinguishing characteristic of infection, although studies have only been performed in traditional cell culture with limited observations in infectedhuman tissues. To study the cVAC, viral and cellular markers that are discrete to their respective compartments …
Human Cytomegalovirus Cytoplasmic Virion Assembly Complex: Structure In Vivo And Role Of Pul103 In Its Biogenesis, Ma Christina Raye Lim
Human Cytomegalovirus Cytoplasmic Virion Assembly Complex: Structure In Vivo And Role Of Pul103 In Its Biogenesis, Ma Christina Raye Lim
Wayne State University Theses
Human Cytomegalovirus (HCMV) is a linear, double stranded DNA virus that causes severe disease in the immunocompromised, and is one of the common cause of congenital disease. Antivirals that are currently available for treatment target the DNA replication of the virus cycle, and are highly toxic. Finding new drug targets, such as proteins responsible for virion assembly and egress, would help to alleviate the disease burden.
HCMV remodels the host cell to form a structure called the cytoplasmic virion assembly complex (cVAC), a site of virion maturation and egress. The first objective of this work is to study the structure …
Hiv Integrase Mechanisms Of Resistance To Raltegravir, Elvitegravir, And Dolutegravir, Kyla Nicole Ross
Hiv Integrase Mechanisms Of Resistance To Raltegravir, Elvitegravir, And Dolutegravir, Kyla Nicole Ross
Wayne State University Theses
ABSTRACT
HIV INTEGRASE MECHANISMS OF RESISTANCE TO RALTEGRAVIR, ELVITEGRAVIR, AND DOLUTEGRAVIR
by
KYLA ROSS
December 2015
Advisor: Dr. Ladislau Kovari
Major: Biochemistry and Molecular Biology
Degree: Master of Science
HIV-1 integrase (HIV-1 IN or IN) is a multimeric enzyme that integrates the HIV-1 genome into the chromosomes of infected CD4+ T-cells. Currently there are three FDA approved HIV-1 IN strand transfer inhibitors (INSTIs) used in clinical practice: raltegravir (RAL), elvitegravir (ELV), and dolutegravir (DTG). The [Q148H], [Q148H, G140S], [Q148R], [Q148R, G140A] and [N155H, E92Q] mutations decrease IN susceptibility to RAL and ELV and may result in therapeutic failure. As an …
Characterization Of Bacteriophages From Environmental Water Samples And The Potential Of Bacteriophages Tailspike Proteins (Tsp) In Bacteria Detection, Gayathri Upeksha Gunathilaka
Characterization Of Bacteriophages From Environmental Water Samples And The Potential Of Bacteriophages Tailspike Proteins (Tsp) In Bacteria Detection, Gayathri Upeksha Gunathilaka
Wayne State University Theses
ABSTRACT
CHARACTERIZATION OF BACTERIOPHAGES FROM ENVIRONMENTAL WATER SAMPLES AND THE POTENTIAL OF BACTERIOPHAGES TAILSPIKE PROTEINS (TSP) IN BACTERIA DETECTION
by
GAYATHRI UPEKSHA GUNATHILAKA
May 2014
Advisor: Dr. Yifan Zhang
Major: Nutrition and Food Science
Degree: Master of Science
The high abundance of phage in the environment and their specificity with the bacteria host make them an excellent tool for bacteria detection. To characterize phage specific to Salmonella and E. coli from water samples in the Southeastern Michigan and to develop a novel bacterial detection method using phage, environmental water samples were collected from rivers and waste water treatment plant (WWTP) …