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Veterinary Medicine

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Sperm

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Full-Text Articles in Life Sciences

Evaluation Of The Effects Of Ghrelin And Metformin On Sperm Parameters, Testosterone Hormones, And Immunohistochemical And Immunofluorescent Markers In An Experimental Diabetes Model, Saadet Belhan, Zübeyi̇r Huyut, Serkan Yildirim, Özlem Ergül Erkeç, Uğur Özdek Oct 2023

Evaluation Of The Effects Of Ghrelin And Metformin On Sperm Parameters, Testosterone Hormones, And Immunohistochemical And Immunofluorescent Markers In An Experimental Diabetes Model, Saadet Belhan, Zübeyi̇r Huyut, Serkan Yildirim, Özlem Ergül Erkeç, Uğur Özdek

Turkish Journal of Veterinary & Animal Sciences

Testicular dysfunction, a severe secondary complication of diabetes, negatively affects reproductive health. Ghrelin is a peptide that plays a role in many metabolic events, such as stimulating growth hormones and regulating appetite, blood pressure, and reproduction. Metformin has antiinflammatory and antioxidant properties and is widely used to treat type 2 diabetes. Therefore, in this study, to investigate the effects of ghrelin and metformin on testicular function in experimental diabetic rats given streptozotocin, placed on a high-fat diet, and that had their sperm parameters evaluated, testosterone hormone, caspase 3 and iNOS expression, and histopathological examinations were performed. In total, 38 Wistar …


Chemical Castration Using An Intratesticular Injection Of Mannitol: A Preliminary Studyin A Rat Model, Mohammad-Ali Maadi, Mehdi Behfar, Amir Rasaei, Ali Shalizar-Jalali, Gholamreza Najafi, Vahid Mohammadi Jan 2021

Chemical Castration Using An Intratesticular Injection Of Mannitol: A Preliminary Studyin A Rat Model, Mohammad-Ali Maadi, Mehdi Behfar, Amir Rasaei, Ali Shalizar-Jalali, Gholamreza Najafi, Vahid Mohammadi

Turkish Journal of Veterinary & Animal Sciences

This study was aimed to evaluated chemical sterilization using an intra-testicular injection of mannitol 20% in rats. Fifty male Wistar rats were divided into five groups, including 1) intact, 2) surgical castration and, 3 to 5) intra-testicular injections of normal saline, hypertonic saline, and mannitol, respectively. The baseline testosterone levels were measured. Surgical castration was performed in group 2. Intra-testicular injections were performed in groups 3, 4, and 5. On day 60, the testosterone level was evaluated. Then, sperm count, viability, motility, morphological abnormalities and DNA damage were studied. Gonadosomatic index was recorded on day 60. Serum testosterone level was …


Effects Of Different Extenders And Additives On Liquid Storage Of Awassi Ram Semen, Ömer Varişli, Abdullah Taşkin, Numan Akyol Jan 2018

Effects Of Different Extenders And Additives On Liquid Storage Of Awassi Ram Semen, Ömer Varişli, Abdullah Taşkin, Numan Akyol

Turkish Journal of Veterinary & Animal Sciences

The aim of this study was to evaluate the effects of different extenders and additives on ram semen during liquid storage and to demonstrate the role of oxidative stress parameters on this process. In the present study, ejaculates taken by artificial vagina twice a week from 4 rams during the breeding season were used. They were mixed and used if motility and viability were above 70% and there was a 95% intact acrosome. The semen specimens were diluted by Tris-citrate-glucose (TRIS), Tris-TES (TEST), HEPES-buffered Tyrode lactate (TL-HEPES), and Dulbecco?s phosphate-buffered saline (PBS) extenders supplemented with different additives [centrifuged egg yolk, …


Investigation Of Serum Testosterone Level, Scrotal Circumference, Body Mass,Semen Characteristics, And Their Correlations In Developing Mis Lambs, Nevena Maksimovic, Slavca Hristov, Branislav Stankovic, Milan P. Petrovic, Cvijan Mekic, Dragana Ruzic-Muslic, Violeta Caro-Petrovic Jan 2016

Investigation Of Serum Testosterone Level, Scrotal Circumference, Body Mass,Semen Characteristics, And Their Correlations In Developing Mis Lambs, Nevena Maksimovic, Slavca Hristov, Branislav Stankovic, Milan P. Petrovic, Cvijan Mekic, Dragana Ruzic-Muslic, Violeta Caro-Petrovic

Turkish Journal of Veterinary & Animal Sciences

The aim of this study was to investigate changes of serum testosterone, scrotal circumference, body weight, and semen characteristics in a Meat Institute Sheep (MIS) population during their developing period (between 3 and 17 months). Correlative relationships among parameters were also tested. Body weight and scrotal circumference were measured monthly, blood samples for testosterone levels were taken bimonthly (3-17 months), and semen samples were collected by an electroejaculator between the ages of 9 and 17 months. The analysis showed that the average serum testosterone levels ranged from 1.83 to 13.28 ng/mL and significantly depended on the age of the lambs …


Dimensional Characteristics Of Spermatozoa Of Nili-Ravi Buffalo Bulls: Seasonaland Climatic Influences, Sajid Hameed, Saima Masood, Hafsa Zaneb, Muhammad Sarwar Khan, Muhammad Younis, Muhammad Avais, Muti Ur Rehman Khan Jan 2016

Dimensional Characteristics Of Spermatozoa Of Nili-Ravi Buffalo Bulls: Seasonaland Climatic Influences, Sajid Hameed, Saima Masood, Hafsa Zaneb, Muhammad Sarwar Khan, Muhammad Younis, Muhammad Avais, Muti Ur Rehman Khan

Turkish Journal of Veterinary & Animal Sciences

This study was conducted in 2 different climatic zones of Punjab Province of Pakistan, the Sahiwal and Bahawalpur divisions, simultaneously for the period of 1 year divided into seasons. Seven adult Nili-Ravi buffalo breeding bulls from each division were used. Dimensional characteristics of spermatozoa were determined. Season had a nonsignificant effect on the sperm head length in both divisions and a nonsignificant difference was also observed between bulls of the Sahiwal and Bahawalpur divisions. The buffalo bulls of both divisions exhibited significantly increased sperm head breadth in autumn, whereas a nonsignificant difference was observed between divisions. Sperm head shape values …


Molecular, Cellular, And Physiological Determinants Of Bull Fertility, Alexis Marie Parisi, Shannon Kate Thompson, Abdullah Kaya, Erdoğan Memi̇li̇ Jan 2014

Molecular, Cellular, And Physiological Determinants Of Bull Fertility, Alexis Marie Parisi, Shannon Kate Thompson, Abdullah Kaya, Erdoğan Memi̇li̇

Turkish Journal of Veterinary & Animal Sciences

Bull fertility, defined as the ability of the spermatozoon to fertilize and activate the ovum and then support embryonic and even fetal development, is a crucial factor influencing animal reproduction and production efficiency. Despite significant influences of fertility on herd genetic improvement and efficient cattle production, the mechanisms regulating this phenotype and reliable biomarkers associated with bull fertility are poorly defined. Specifically, there is a broad lack of knowledge in bull fertility. In this paper, bull fertility is reviewed within the context of animal systems physiology, and system biology markers associated with bull fertility are provided. This mini-review is intended …


Cholesterol Efflux From Sperm: Approaches And Applications, Zahid Naseer, Ejaz Ahmad, Meli̇h Aksoy Jan 2014

Cholesterol Efflux From Sperm: Approaches And Applications, Zahid Naseer, Ejaz Ahmad, Meli̇h Aksoy

Turkish Journal of Veterinary & Animal Sciences

Following ejaculation, sperm appear morphologically mature, but functionally they are inactive. To attain functional capability or capacitation in vivo, sperm must reside in the female reproductive tract for a certain period of time before fertilization. In the female reproductive tract, cholesterol efflux from the sperm plasma membrane is considered vital for the initiation of capacitation. A number of in vitro studies have used biological and nonphysiological means to understand the phenomenon of cholesterol efflux and capacitation. In this review, we present a short overview of the role of cholesterol efflux in regulating sperm capacitation using various agents. This review provides …


Antioxidant Supplementation And Purification Of Semen For Improved Artificial Insemination In Livestock Species, Peter Petruska, Marcela Capcarova, Peter Sutovsky Jan 2014

Antioxidant Supplementation And Purification Of Semen For Improved Artificial Insemination In Livestock Species, Peter Petruska, Marcela Capcarova, Peter Sutovsky

Turkish Journal of Veterinary & Animal Sciences

Improving sire reproductive performance in artificial insemination (AI) programs and maximizing the use of sires with valuable genomes are high on the list of priorities for livestock breeders in the United States and worldwide. While fixed-time AI and accurate estrous detection have already ameliorated the reproductive management of cows and sows, substantial gains remain to be made in the selection and utilization of male animals for field AI. In addition to better, more objective evaluation of breeding soundness and semen quality, numbers of spermatozoa per AI dose could be reduced and numbers of AI doses per collection could be increased …


Effect Of Dietary Energy Intake And Somatotropin Administration After Weaning On Growth Rate And Semen Characteristics Of Granadina Goat Bucks, Miguel Mellado, Cesar A. Meza-Herrera, Jose R. Arevalo, Jose E. Garcia, Francisco G. Veliz Jan 2012

Effect Of Dietary Energy Intake And Somatotropin Administration After Weaning On Growth Rate And Semen Characteristics Of Granadina Goat Bucks, Miguel Mellado, Cesar A. Meza-Herrera, Jose R. Arevalo, Jose E. Garcia, Francisco G. Veliz

Turkish Journal of Veterinary & Animal Sciences

Twenty-eight growing Granadina goat bucks, divided into 4 groups of 7, in a 2 × 2 factorial arranged design, were used to study the effect of post-weaning levels of dietary energy and administration of recombinant bovine somatotropin (rbST) on growth and semen characteristics. Every 14 days, bucks were offered sustenance at rates of either 1.0 (standard diet, 2.36 Mcal EM kg^{-1}; 15% CP) or 1.25 (increased diet, 2.95 Mcal EM kg^{-1}; 18% CP) times the requirements for growth energy and protein, for a total of 99 days and in combination with or without subcutaneous administration of 125 mg rbST. The …


Comparison Of The Fatty Acid Compositions Of Egg, And Sperms Of Brood Stock Rainbow Trout (Oncorhynchus Mykiss), N. Mevlüt Aras, H. İbrahi̇m Hali̇loğlu, Muhammed Atamanalp, E. Mahmut Kocaman Jan 2003

Comparison Of The Fatty Acid Compositions Of Egg, And Sperms Of Brood Stock Rainbow Trout (Oncorhynchus Mykiss), N. Mevlüt Aras, H. İbrahi̇m Hali̇loğlu, Muhammed Atamanalp, E. Mahmut Kocaman

Turkish Journal of Veterinary & Animal Sciences

The aim of this research was to compare the fatty acid composition of egg, sperm and diet of 3-year-old brood stock rainbow trout. The results showed that there were statistically significant (P < 0,01) differences between egg, sperm and diet levels in terms of SFA, MUFA, PUFA (n-3, n-6) and EPA/DHA, which are known as important agents for fish nutrition.


Investigation Of In Vitro Effects Of Fluoride On Bovine Sperm, Sadetti̇n Tanyildizi, Tanzer Bozkurt Jan 2002

Investigation Of In Vitro Effects Of Fluoride On Bovine Sperm, Sadetti̇n Tanyildizi, Tanzer Bozkurt

Turkish Journal of Veterinary & Animal Sciences

The in vitro effects of various concentrations of sodium fluoride on the percentages of motile spermatozoa and abnormal spermatozoa with the hyaluronidase activities of semen were investigated. Holstein bulls (n=20), aged between 4 and 5 years, were used in this study. The semen samples obtained from all animals were divided into five parts and one of them was used as the control sample. Sodium fluoride solutions were prepared at concentrations of 30, 60, 120 and 240mM and then these solutions were mixed 1:1 with semen samples to determine semen hyaluronidase activity, sperm motility and morphological abnormality of spermatozoa at 5, …


An Investigation Of The Effects Of Ivermectin On Blood Serum, Semen Hyaluronidase Activities And Spermatological Characteristics In Sheep, Sadetti̇n Tanyildizi, Tanzer Bozkurt Jan 2002

An Investigation Of The Effects Of Ivermectin On Blood Serum, Semen Hyaluronidase Activities And Spermatological Characteristics In Sheep, Sadetti̇n Tanyildizi, Tanzer Bozkurt

Turkish Journal of Veterinary & Animal Sciences

This study was carried out to investigate the effects of ivermectin on the hyaluronidase activities of blood serum and semen and on the sperm characteristics in sheep. In this investigation 18 sheep, at the age of 2 years and weighing between 50-60kg were used. After the administration of ivermectin subcutanously at a dose of 0.2 mg/kg, semen and blood samples were taken at different times. The hyaluronidase activities of blood serum and semen samples in sheep were determined to increase significantly (p