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Activity Of 3\Beta-Hydroxysteroid Dehydrogenase Associated With Progesterone Production In Bovine Granulosa Cells Cultured Under Different Concentrations Of Serum, Insulin-Like Growth Factor I, And Gonadotropin, Özkan Şi̇mşek, Monika Mihm
Activity Of 3\Beta-Hydroxysteroid Dehydrogenase Associated With Progesterone Production In Bovine Granulosa Cells Cultured Under Different Concentrations Of Serum, Insulin-Like Growth Factor I, And Gonadotropin, Özkan Şi̇mşek, Monika Mihm
Turkish Journal of Veterinary & Animal Sciences
Three-\beta-hydroxysteroid dehydrogenase (3\beta-HSD) is the enzyme responsible for progesterone production. This study aimed to determine whether 3\beta-HSD activity can be shown to reflect progesterone production by granulosa cells cultured under different serum conditions and follicle stimulating hormone (FSH), luteinising hormone (LH), and insulin-like growth factor I (IGF-I) concentrations. Large bovine follicles were dissected from abattoir ovaries to recover granulosa cells. Cells were washed, stained for viability, and plated for 48 h in basic medium with or without 5% foetal calf serum (FCS). Subsequently, cells were exposed to FSH (1 ng/mL), LH (10 or 100 ng/mL), or FSH (1 ng/mL) + …
Repeated Superovulation Treatments In Kivircik Ewes During Breeding And Nonbreeding Seasons, Burcu Üstüner, Seli̇m Alçay, Yavuz Nak, Zekari̇ya Nur, Deni̇z Nak, Bi̇lgi̇ner Tuna, Gözde Şi̇mşek, Hakan Sağirkaya
Repeated Superovulation Treatments In Kivircik Ewes During Breeding And Nonbreeding Seasons, Burcu Üstüner, Seli̇m Alçay, Yavuz Nak, Zekari̇ya Nur, Deni̇z Nak, Bi̇lgi̇ner Tuna, Gözde Şi̇mşek, Hakan Sağirkaya
Turkish Journal of Veterinary & Animal Sciences
The present study compared superovulation and embryo recovery rates applied repeatedly during breeding and nonbreeding seasons in the same 20 Kivircik sheep. Trials 1 and 3 were performed during the breeding season and the trial 2 was performed in the nonbreeding season. We synchronized the sheep's estrus cycles and induced a superovulatory response with porcine follicle stimulating hormone (pFSH) injections. Natural mating took place 12 h after the last FSH injection. The embryos were collected by laparotomy 7 days after natural mating. There was no significant difference between the number of corpora lutea (CL) counted following the 1st (breeding) and …