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Histomonas Elisa, Jessica L Martinez
Histomonas Elisa, Jessica L Martinez
EURēCA: Exhibition of Undergraduate Research and Creative Achievement
Histomonas meleagridis is a protozoan parasite of avians and is the causative agent in Histomonosis, commonly known as Blackhead Disease. Current methods for diagnosing the presence of H. meleagridis are limited to parasite culture or Polymerase Chain Reaction (PCR) to amplify target DNA. This project aims to develop an enzyme-linked immunosorbent assay (ELISA) for faster and more sensitive diagnosis of Histomonas infections. Cultures of H. meleagridis parasites were purified, and surface antigens were extracted using a spectrum of chemical solutions. The various antigen solutions were subjected to an ELISA, with serum from birds immunized for H. meleagridis as positive controls. …
Volume 13, Number 1 (Spring/Summer 2017), Ut Institute Of Agriculture
Volume 13, Number 1 (Spring/Summer 2017), Ut Institute Of Agriculture
Tennessee Land, Life and Science Magazine
Issue Highlights:
- The four pillars of the Institute of Agriculture
- Alumna forges partnership amid Waldo Canyon fire
- Fishery biologists revive a river
Volume 13, Number 2 (Fall/Winter 2017), Ut Institute Of Agriculture
Volume 13, Number 2 (Fall/Winter 2017), Ut Institute Of Agriculture
Tennessee Land, Life and Science Magazine
Issue Highlights:
- The four pillars of the Institute of Agriculture
- Alumna forges partnership amid Waldo Canyon fire
- Fishery biologists revive a river