Life Sciences Commons^™

The Comparison Of Different Wetland Fish Assemblages Over Time, Robert Edward Adelstein

Theses, Dissertations and Capstones

Wetlands provide essential ecosystem services. Historically, we have drained and filled 73% of wetlands for agricultural use throughout the United States from the 1780s to the 1980s (Dahl, 1990). A nationwide focus on restoring wetlands has since occurred. Literature on restored/mitigated wetlands is rife with examples that do and do not support the same ecosystem services as natural wetlands (Langston, 1997; Meil, 2014). Restoration of wetlands occurred at the Green Bottom Wildlife Management Area (GBWMA) over several decades. Various sections of the wetland were classified by age, water depth, and vegetation. One hypothesis was that differences in fish assemblage would …

Assessing The Unionid Assemblage Of The Robert C. Byrd Pool, Ohio River, Ethan Hunter Bellamy

Theses, Dissertations and Capstones

Unionid mussels are the most imperiled taxa in the United States and are vastly understudied. The entire Robert C. Byrd (RCB) pool of the Ohio River is the study area. I selected sites from the 2019 and 2013 ORSANCO RCB pool assessments that utilize random site selection across the pool. I used SCUBA to survey and collect data on unionid diversity, reproduction, and habitat. I collected 1,083 individuals over 19 species in RCB pool. I compared results from RCB pool survey to two similar surveys conducted in Greenup Pool. Both richness and abundance in the Upper Section of Greenup Pool …

Characterizing The Vegetation And Effects Of Climate Change On Parris Island, A Sea Island Ecosystem, Cody Hart Goodson

Theses, Dissertations and Capstones

Coastal habitats provide many ecosystem services, protecting coastlines from storm surges and erosion, diminishing the effects of eutrophication, sequestering large amounts of carbon, and acting as vital wildlife habitat. Sea-level rise and increased storm surge intensity associated with climate change are increasingly disrupting coastal habitats. These disturbances can shift environmental gradients that drive the zonation of coastal vegetation types, driving habitat conversion. Monitoring coastal habitat conversion can improve our understanding of the dynamic effects of climate change on these landscapes. Therefore, our objectives for chapter 1 were to identify and describe the distributions of vegetation types present on Marine Corps …

An Analysis Of The Preferences, Morphometrics, And Survey Efficacy Of The Freshwater Mussel Community In The Greenup Pool, Ohio River, Jacob Miller

Theses, Dissertations and Capstones

The Greenup Pool is one of the most unique pools of the Ohio River in terms of habitat richness and anthropogenic impact both historical and current. The Greenup Pool spans 61.8 miles reaching from Greenup Dam in Greenup, KY to Robert C. Byrd Dam in Apple Grove, WV. We surveyed 17 sites to assess mussel communities within the Greenup Pool using the 2017 West Virginia Mussel Survey Protocol. At these sites, six 100-meter transects were surveyed for surface and subsurface mussels. Each transect was divided into 10-meter intervals. These surveys resulted in 4,041 live individuals from 21 species, including eight …

Assessing The Toxicity Of A Reconstituted Water Simulating Streams Influenced By Mountaintop Mining In Central Appalachia, Benjamin David Browning

Theses, Dissertations and Capstones

Freshwater ecosystems in Central Appalachia experience increased concentrations of manganese (Mn) and total dissolved solids from the runoff of surface mines and valley fills. Biological communities have been impacted by these surface mining operations and it has been suggested that the increase in total dissolved solids may contribute to these negative effects, but standard laboratory toxicity tests have not found increased concentrations of total dissolved solids to have such negative effects as seen in the field. The elevated total dissolved solids in mining influenced streams may only be toxic in conjunction with another toxicant that is presence in these systems …

An Exceptionally Small New Polycotylid Plesiosaur (Reptilia: Sauropterygia) With Raptorial Eyes From The Western Interior Seaway Of North America, Robert O’Brien Clark

Theses, Dissertations and Capstones

Polycotylidae is a family of plesiosaurian marine reptiles that evolved during the Early Cretaceous and radiated into multiple genera during the Late Cretaceous, achieving a worldwide distribution. Derived polycotylids of the subclade Polycotylinae have a gracile and elongated rostrum, homodont dentition, an extended mandibular symphysis, and foreshortened temporal fenestrae. In this thesis, I describe a small and highly derived new polycotylid taxon based on three specimens from the Campanian of the Western Interior Seaway in North America. A high number of maxillary teeth, fused neural arches, propodials with well-defined facets, and heavily remodeled cortical bone indicate the specimens are adults, …

Foraging Strategy Plasticity In Fiordland Penguins (Eudyptes Pachyrhynchus): A Stable Isotope Approach, Jeffrey Wayne White

Theses, Dissertations and Capstones

Foraging ecology in the crested penguins (genus: Eudyptes) is an active area of research, with new techniques rapidly increasing our understanding of these charismatic species. The most common techniques to assess foraging ecology include stomach content analysis, fecal DNA analysis, stable isotope analysis, tracking, and video loggers. Here we review dietary research on all 8 taxa within the genus Eudyptes to identify gaps in our current knowledge. However, foraging studies that assess dietary segregation require a method for quickly and accurately sexing penguins in the field. Obvious sexual dimorphism in plumage is largely absent in penguins leaving behavioral cues …

Laboratory Investigation On The Effects Of Conductivity On The Sensitive Early Life Stages Of Fishes From The Appalachian Region, Logan Ryan Beach

Theses, Dissertations and Capstones

While it is known there is a link between land disturbance and elevations in ionic constituents in streams, the relationship between elevated conductivity and aquatic taxa impairment is harder to define. Multiple field studies demonstrating correlations between conductivity and fish or benthic macroinvertebrate communities have not described the mechanisms of impairment and impairment has not been demonstrated with traditional toxicity testing. In an effort to explore more sensitive sub-lethal endpoints for evaluation of instream effects of mining effluent, chronic toxicity testing was conducted on eggs and early life stages of trout species and the fathead minnow, utilizing a simulated mining …

Eastern Diamondback Rattlesnake (Crotalus Adamanteus) Ambush Site Selection In Coastal Saltwater Marshes, Emily Rebecca Mausteller

Theses, Dissertations and Capstones

The eastern diamondback rattlesnake (Crotalus adamanteus; EDB) is a species of conservation concern associated with the imperiled longleaf pine-grassland ecosystem. The longleaf pine ecosystem is characterized by an open canopy and rich ground cover. Researchers have speculated that the vegetation structure of salt marshes may serve as a surrogate habitat for longleaf pine savannas. Although these marshes have little topography, they provide a heterogeneous landscape with patches of mud flats, sandy hard marsh along upper tidal areas, and salt marsh hummocks throughout. I used radio telemetry to monitor free-ranging EDBs on a South Carolina sea island. The goal of my …

Modeling Chlorophyll Concentrations On The Ohio River Using Remotely Sensed Data, Thaddaeus Stephen Tuggle

Theses, Dissertations and Capstones

Traditional direct water quality methodologies limit the ability to spatially and temporally predict algal blooms in lotic systems due to the size and characteristics of large river systems. Algal blooms potentially can be predicted by knowing the spatial and temporal patterns of change in cyanobacteria concentrations at large scales. Remote sensing studies investigating freshwater algal blooms, some known to secrete harmful toxins, are primarily conducted on lentic systems while large lotic systems are greatly ignored. In this study I developed a chlorophyll concentration estimation model for the Ohio River using a satellite remote sensing approach. Ground-truth water quality measures, including …

Freshwater Mussels Of The Greenup Navigational Pool, Ohio River, With A Comparison To Fish Host Communities, Mitchell David Kriege

Theses, Dissertations and Capstones

The Ohio River was historically a free-flowing system with diverse fish and freshwater mussel communities. Heavy industrialization, erosion from deforestation, and wide scale damming during the early-mid 20th century decimated riverine life. While mussel declines are well documented in the United States, in big river systems, freshwater mussel populations are poorly understudied. This thesis project mapped the mussel communities and site-specific sediments of the Greenup pool in the Ohio River for comparison to 2016 nighttime electrofishing data, provided by ORSANCO. Qualitative SCUBA surveys were performed at 18 randomly selected sites and two fixed sites between July and September. Each site …

Amphibians Among Road-Rut Pools In West Virginia, Abby L. Sinclair

Theses, Dissertations and Capstones

As anthropogenic environmental stressors such as urbanization continue to increase, it is necessary to understand their impact on amphibian ecology. Due largely to their biphasic life history amphibians are negatively impacted by the destruction of lotic habitats i.e., wetlands for the creation of roads and urban centers. I examined the effects of three human-made road rut pools on the reproductive success and species richness of amphibians in southwest West Virginia from May to October 2012. I employed three detection techniques: drift fence pitfall trap arrays, funnel traps and dipnet sampling. Additionally, habitat covariates were taken (i.e., water depth) at each …

A Comparative Analysis Of The Metabolism And Energetics Of Darters (Percidae), Emma Kirsten Kist

Theses, Dissertations and Capstones

Ecological niche theory suggests two species cannot live in the same ecological niche and differences should exist among species that appear to cohabitate. Variations in physiology and behavior that potentially enable species coexistence can be reflected in metabolism. This study investigated coexisting darter fishes by measuring the metabolism of greenside (Etheostoma blennioides) and variegate (E. variatum) darters over 48 h using intermittent-flow respirometry. Activity was analyzed using time-lapse videos. E. blennioides mean metabolic rate (154.64 ± SE 52.54 mg O2·kg-1 ·hr-1 ; n=14; p=0.0006) was significantly greater than and varied more than E. variatum’s mean rate (92.51 ± SE 32.70 …

An Assessment Of West Virginia Watersheds Through The Use Of Geographic Information System (Gis), Nathan Thomas Hoxie

Theses, Dissertations and Capstones

Analysis of West Virginia Department of Environmental Protection (WVDEP) agency’s Stream Condition Index and habitat assessment data compared two cycles of data. Geographic Information System (GIS) was used to develop a kriging model to project scores. Scores were calculated for Hydrologic Unit Code (HUC) eight and ten using Zonal statistics. The Getis-Ord Gi Star was used to identify high and low value clusters. Land use and impervious surface data was gathered from the United States Geological Survey (USGS) to assess impacts to watersheds. The results for WVSCI and habitat assessment showed that there was an improvement between cycles. Negative WVSCI …

Testing The Efficacy Of Anuran Callback Surveys, Matthew S. Grisnik

Theses, Dissertations and Capstones

Monitoring programs often suffer from imperfect detection resulting in skewed population estimates, biased estimates of changes in occupancy over time, and can result in an underestimated proportion of area occupied (PAO) by a species. To increase the detection probability, researchers must increase sampling both spatially and temporally. Callback surveys are an active form of sampling that have been used to monitor many avian and mammal species. During callback surveys, the call of a conspecific male is projected with the intention of increasing probability of detection by eliciting a response from territorial males. These methods work for organisms that establish breeding …

Population Level Responses To Direct Application Liming In Gyrinophilus Porphyriticus, Shelby Renea Timm

Theses, Dissertations and Capstones

Direct application liming (DAL) has been used to neutralize acidified streams to restore aquatic biota. This mitigation technique has been used globally for decades, yet little data exist on its effects on amphibian populations. My study investigated the effects of liming on amphibians by measuring variability in life histories of larval Gyrinophilus porphyriticus. I collected larvae from six streams in the Monongahela National Forest, West Virginia. I examined the effects of DAL on age structure, and I failed to detect a treatment effect. I used ANCOVAs to examine differences in body condition, body size, and gape size. I observed that …

Examining The Influence Of Mating Systems On Testes Size In Salamanders, Howard James Stanton Ii

Theses, Dissertations and Capstones

Sperm competition theory predicts that relatively larger testes sizes evolve in animals with polygamous mating systems compared to those in monogamous mating systems due to sperm competition. Whereas intensity of sperm competition is the significant predictor of testes sizes in other taxa such as mammals, frogs, birds, insects, and fish, in salamanders the intensity of male-male competition in the transfer of spermatophores to females is predicted to be a critical factor. This is because males have to deposit more spermatophores to secure reproductive pay-off under higher intensity of male-male competition. I hypothesized that salamander species that breed explosively as groups …

Quantitative Reconstruction And Two-Dimensional, Steady Flow Hydrodynamics Of The Plesiosaur Flipper, Mark Cruz Deblois

Theses, Dissertations and Capstones

Plesiosaurs are a group of extinct marine reptiles that thrived during the Mesozoic Era. They are unique for swimming with two hydrofoil-shaped flippers. Penguins, sea turtles, and cetaceans all have hydrofoil shaped flippers but penguins and sea turtles only use the front pair to produce thrust and cetaceans use their tail flukes. Consequently, the mode of swimming for plesiosaurs has long been debated. However, a quantitative study of the hydrodynamic properties of the flippers, which would constrain inference about their mode of swimming, has not yet been done. The main reason is that the trailing edge of the plesiosaur flipper …

Dietary Preference Of The Queensnake (Regina Septemvittata), Timothy J. Brust

Theses, Dissertations and Capstones

The Queensnake (Regina septemvittata) is a small secretive water snake found throughout the eastern United States. Once common, their numbers have declined to the extent that they are now threatened throughout most of their range, largely the result of pollutant-based reduction in prey species. These snakes are assumed to eat molted crayfish exclusively. For some common crayfish species, molting happens only twice a summer during a two- week period. It has not been documented if Queensnakes eat anything besides crayfish on a regular basis. The purpose of this study was to determine the prey preference of Queensnakes with particular focus …

Chronic Toxicity Testing In Mining Influenced Streams Of West Virginia, Leah J. Bitzer

Theses, Dissertations and Capstones

Whole effluent toxicity (WET) tests have become a common tool in the evaluation of effluent for discharge acceptability. In this study, four years of toxicity data from 119 sampling locations were analyzed to determine relationships with ions and conductivity as indicators of toxicity. West Virginia Stream Condition Index (WVSCI) scores were also examined to evaluate correlations between stream scores, conductivity, and IC25 endpoints from toxicity results. Conductivity was not an indicator of toxicity in the range of conductivities tested. Streams dominated by mining effluent sometimes exhibited toxicity to Ceriodaphnia dubia; however, toxicity was not found to be related to ionic …

A Virtual Phytosaur Endocast And Its Implications For Sensory System Evolution In Archosaurs, Waymon Holloway

Theses, Dissertations and Capstones

Due to the overall morphological similarities between the Triassic archosaurs of the order Phytosauria and extant crocodilians, most studies have assumed that the two shared similar lifestyles. Many studies involving phytosaurs have focused on the external cranial morphology of various taxa. Internal cranial anatomy has received relatively little attention. As a result, comparative morphology studies of the braincases interior, or endocast, of phytosaurs are an area of potential exploration. Just as modern medical Xray computed tomography (CT) can be used to create three-dimensional images of internal structures of living subjects, such technology offers a non-invasive means of studying the internal …

Plesiosaur Body Shape And Its Impact On Hydrodynamic Properties, Courtney D. Richards

Theses, Dissertations and Capstones

Despite the variability of cross-sectional body shape within Plesiosauria, its impact on plesiosaur buoyancy and stability has never been investigated. This study focused on Tatenectes, Cryptoclidus, and Muraenosaurus due to their variable body morphologies. Reconstructions were created based on measurements and photographs from fossil remains. The ability of computer models, based upon the reconstructions, to reach equilibrium after submersion, sink via lung deflation, and recover from a lateral roll was tested. For the computer models, Muraenosaurus was replaced with Thalassomedon, which had a similar morphology. Cryptoclidus and Thalassomedon recovered from submersion faster than Tatenectes. All models achieved negative buoyancy with …

The Little Coal River Improvement Project, West Virginia: An Initial Study Of Sediment, Bacteria And Benthic Macroinvertebrates, Emily Vargo

Theses, Dissertations and Capstones

The Little Coal River, located in southern West Virginia, has undergone many changes over the last few years in an effort to reverse the negative effects of various point source and non-point source pollutions. Marshall University, in cooperation with the West Virginia Department of Environmental Protection, Army Corp of Engineers, and the Coal River Group, has designed and implemented a plan for rehabilitation known as the Little Coal Improvement Project. Several improvement structures were strategically installed within the Little Coal River during the summer of 2007. The functions of these structures include sediment flushing and overall habitat improvement. Monitoring these …

Phylogenetic Analysis Of Blacknose Dace (Rhinichthys) In West Virginia Streams, Samantha Taylor

Theses, Dissertations and Capstones

Blacknose dace (Rhinichthys) are one of the most common cyprinid fishes in eastern North America. They also have been a topic of debate for over 30 years because morphology-based systematics has failed to clearly define their taxa. Taxonomists classify the complex into two species and one subspecies: the eastern form, R. atratulus atratulus; and the western form R. obtusus obtusus, and southern form R. obtusus meleagris. This research uses the mitochondrial cytochrome b gene and genomic RAG 2 gene in a phylogenetic analysis to help clarify species relations according to differences between each current species. Maps have been created to …

Dopamine Modulates The Lateral Giant Neuron And Serotonergic Facilitation In Crayfish, Joshua Scott Titlow

Theses, Dissertations and Capstones

The lateral giant (LG) neural circuit is a model system used to study the function of individual neurons. The LG circuit is part of a tail flip escape reflex that is a defensive behavior for crayfish. This thesis begins by addressing the effects of dopamine (DA), a neurotransmitter involved in normal and abnormal behaviors of most animals. Here it is shown that dopamine decreases the excitability of the LG neuron, a trigger for the escape reflex. An electrophysiology protocol was used to mimic sensory input to the LG neuron. Stimulating a sensory nerve in the last ganglion with an electrode …

The Natural History & Distribution Of Riverine Turtles In West Virginia, Linh Diem Phu

Theses, Dissertations and Capstones

Turtles are unique evolutionary marvels that evolved from amphibians and developed their protective shelled form more than 200 million years ago. In West Virginia, there are 10 native species of turtles, 9 of which are aquatic. Most of these aquatic turtles feed on carrion and dead plant matter, in the water and essentially "clean" our water systems. Turtles are long-lived animals with sensitive life stages that can serve as both long-term and short-term bioindicators of environmental health. With the increase in commercial trade, habitat fragmentation, degradation, destruction, there has been a marked decline in turtle species. The need for well …

The Coal River Basin: A 2009 Water Budget Study, R. Dale Biller

Theses, Dissertations and Capstones

The Coal River Watershed covers an area of some 890 square miles in southern West Virginia of which 863 square miles was investigated in this study. Both online and field data were collected over period from January 13, 2009 to January 13, 2010. The basin was studied as a closed system having an input, which was precipitation, and outputs, rainfall interception, evapotranspiration, and stream discharge that was separated into base flow and overland flow.

The effective rainfall of the watershed was calculated using the Thiessen polygon method to be 42 inches for the year, 48% of that water discharged by …

Determining The Distribution Of Antibiotic Resistant And Fecal Indicator Bacteria In The Ohio River, Caitlin Nicole Swecker

Theses, Dissertations and Capstones

The Ohio River extends 981 miles from Pittsburgh, PA to Cairo, Ill providing drinking water to over three million people, a natural habitat for aquatic life, a public recreation resource, a major transportation route, and a source of water for industry. The Guyandotte River is a highly impacted tributary emptying into the Ohio River in Huntington, WV. The objectives of this study were to determine if sediment load is correlated to the number of antibiotic resistant bacteria (ARB) and determine if a single surface sample is a sufficient representative measurement of ARB populations in a large river. In 2007, subsurface …

Distribution Of Woodland Salamanders Of The Valley And Ridge In West Virginia, H. Reid Downer

Theses, Dissertations and Capstones

Two terrestrial woodland salamanders of the genus Plethodon, P. punctatus and P. virginia, are endemic to the Valley and Ridge Physiographic Province in West Virginia and Virginia and occupy limited geographic ranges. Two common and widespread species, P. cylindraceus and P. cinereus, also inhabit this region. To evaluate the distribution and habitat of these terrestrial salamanders I surveyed wooded ridges and slopes throughout the geographic range of the two endemic species by conducting daytime cover object searches and nocturnal visual encounter surveys. From March through November 2008, I recorded the presence of 321 woodland salamanders at 91 sites. I used …

Terrestrial Habitat Use Of Marbled Salamanders Ambystoma Opacum: A Site Specific Approach, Frank Piccininni

Theses, Dissertations and Capstones

Habitat destruction, fragmentation and degradation have contributed heavily to the decline of amphibian populations such as Marbled Salamanders (Ambystoma opacum). Often, ambystomatid water resources are conserved without consideration for the equally important terrestrial habitat. This is partly due to a lack of information regarding the relationship between ecological succession, plant community composition, microhabitat and salamander abundance. Three sampling transects consisting of drift fence arrays, vegetation assessments and microhabitat surveys were extended 100 m into the terrestrial habitat surrounding a seasonal wetland at Beech Fork State Park in Wayne County, West Virginia. Principal components analysis was used to identify …