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Life Sciences Commons

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Terrestrial and Aquatic Ecology

Online Theses and Dissertations

Theses/Dissertations

2019

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The Use Of Environmental Dna For The Detection Of Palaemonias Ganteri (Hay, 1901), A Federally Endangered Cave Species, Andrew Joseph Stump Jan 2019

The Use Of Environmental Dna For The Detection Of Palaemonias Ganteri (Hay, 1901), A Federally Endangered Cave Species, Andrew Joseph Stump

Online Theses and Dissertations

Kentucky’s limestone caves and karst water systems are an ecologically important part of its natural heritage and home to many unique subterranean species. In addition to being geologically interesting, it is also home to one, of only three, extant North American atyids: the federally endangered Kentucky Cave Shrimp (Palaemonias ganteri, Hay 1901). However, access for monitoring and management objectives involving this species and other cave inhabitants can be difficult, highly contingent upon environmental conditions, and time consuming with low yields using traditional techniques. Advancements in metabarcoding and Next Generation Sequencing (NGS) technologies provide tools that may allow researchers and managers …