Life Sciences Commons^™

The Aboveground And Belowground Growth Characteristics Of Juvenile Conifers In The Southwestern United States, N. L. Pirtel, R. M. Hubbard, J. B. Bradford, T. E. Kolb, M. E. Litvak, Scott Abella, S. L. Porter, Matthew Petrie

Life Sciences Faculty Research

Juvenile tree survival will play an important role in the persistence of coniferous forests and woodlands in the southwestern United States (SWUS). Vulnerability to climatic and environmental stress declines as trees grow, such that larger, more deeply rooted juveniles are less likely to experience mortality. It is unclear how juvenile conifers partition the aboveground and belowground components of early growth, if growth differs between species and ecosystem types, and what environmental factors influence juvenile carbon allocation above- or belowground. We developed a novel data set for four juvenile conifer groups (junipers, piñon pines, ponderosa pines, firs; 1121 juveniles sampled, 221 …

Microbiome Shifts Associated With The Introduction Of Wild Atlantic Horseshoe Crabs (Limulus Polyphemus) Into A Touch-Tank Exhibit, Ariel D. Friel, Sean A. Neiswenter, Cale O. Seymour, Lauren Rose Bali, Ginger Mcnamara, Fabian Leija, Jack Jewell, Brian P. Hedlund

Life Sciences Faculty Research

The Atlantic horseshoe crab (Limulus polyphemus) is a common marine aquarium species and model organism for research. There is potential monetary and conservation value in developing a stable captive population of horseshoe crabs, however, one major impediment to achieving captivity is a lack of knowledge regarding captive diseases. We utilized 16S rRNA gene amplicon sequencing to track changes in the microbiomes of four body locations in three wild-caught (tracked over 14 months in captivity) and three tank-acclimated (>2 years in captivity) adult L. polyphemus in a touch tank at Shark Reef Aquarium at Mandalay Bay in Las Vegas, NV. …

Phytoplankton Community And Algal Toxicity At A Recurring Bloom In Sullivan Bay, Kabetogama Lake, Minnesota, Usa, Victoria G. Christensen, Ryan P. Maki, Erin A. Stelzer, Jack E. Norlan, Eakalak Khan

Civil and Environmental Engineering and Construction Faculty Research

Kabetogama Lake in Voyageurs National Park, Minnesota, USA suffers from recurring late summer algal blooms that often contain toxin-producing cyanobacteria. Previous research identified the toxin microcystin in blooms, but we wanted to better understand how the algal and cyanobacterial community changed throughout an open water season and how changes in community structure were related to toxin production. Therefore, we sampled one recurring bloom location throughout the entire open water season. The uniqueness of this study is the absence of urban and agricultural nutrient sources, the remote location, and the collection of samples before any visible blooms were present. Through quantitative …

Heterogeneity In Surface Sensing Suggests A Division Of Labor In Pseudomonas Aeruginosa Populations, Catherine R. Armbuster, Calvin K. Lee, Jessica Parker-Gilham, Jaime De Anda, Aiguo Xia, Kun Zhao, Keiji Murakami, Boo Shan Tseng, Lucas R. Hoffman, Fan Jin, Caroline S. Harwood, Gerard Cl Wong, Matthew R. Parsek

Life Sciences Faculty Research

The second messenger signaling molecule cyclic diguanylate monophosphate (c-di-GMP) drives the transition between planktonic and biofilm growth in many bacterial species. Pseudomonas aeruginosa has two surface sensing systems that produce c-di-GMP in response to surface adherence. Current thinking in the field is that once cells attach to a surface, they uniformly respond by producing c-di-GMP. Here, we describe how the Wsp system generates heterogeneity in surface sensing, resulting in two physiologically distinct subpopulations of cells. One subpopulation has elevated c-di-GMP and produces biofilm matrix, serving as the founders of initial microcolonies. The other subpopulation has low c-di-GMP and engages in …

Muscular Apoptosis But Not Oxidative Stress Increases With Old Age In A Long-Lived Diver, The Weddell Seal, Kaitlin N. Allen, Jose Pablo Vazquez-Medina, John M. Lawler, Jo-Ann E. Mellish, Markus Horning, Allyson G. Hindle

Life Sciences Faculty Research

Seals experience repeated bouts of ischemia–reperfusion while diving, potentially exposing their tissues to increased oxidant generation and thus oxidative damage and accelerated aging. We contrasted markers of oxidative damage with antioxidant profiles across age and sex for propulsive (longissismus dorsi) and maneuvering (pectoralis) muscles of Weddell seals to determine whether previously observed morphological senescence is associated with oxidative stress. In longissismus dorsi, old (age 17–26 years) seals exhibited a nearly 2-fold increase in apoptosis over young (age 9–16 years) seals. There was no evidence of age-associated changes in lipid peroxidation or enzymatic antioxidant profiles. In pectoralis, 4-hydroxynonenal-Lys (4-HNE-Lys) levels increased …

Limnological Assistance For Lake Mead National Recreation Area: Quarterly Report, Period Ending September 30, 2012, Margaret N. Rees

Limnological Studies

Continuation of Project 1

• The research effort titled Growth of adult quagga mussels within Las Vegas Wash/Las Vegas Bay is ongoing.

Continuation of Project 2

• Additional content was contributed to the future NPS-funded U.S. Geological Survey Circular ‘state-of-the-science’ report for Lakes Mead and Mohave. UNLV performed a new co-editing and coordinating task related to this product as the major focus of this quarter’s effort; the master version of this report is on schedule for delivery to the editorial team during the first week of October 2012.

Modification Activities:

Extended Short-term Continued Limnological Assistance

Quagga Mussel Objectives:

• Most juvenile/adult quagga mussel …

Limnological Assistance For Lake Mead National Recreation Area: Quarterly Report, Period Ending December 31, 2011, Margaret N. Rees

Limnological Studies

Project 1

• Technical assistance with the implementation of the Interagency Monitoring Action Plan (IMAP) for Quagga Mussels is ongoing; remaining analyses are underway with report delivery rescheduled for 05/2012.
• Data have been delivered for Veliger abundance and settlement at different depths of Lake Mead with report delivery re-scheduled for 05/2012.
• Growth of adult quagga mussels within Las Vegas Wash/Las Vegas Bay
• The Interagency Quagga Mussel Meeting was held on 11/17/2011; the next meeting is scheduled for 02/16/2011.

Project 2

• Appendix 7, an addition to the Long-term Limnological and Aquatic Resource Monitoring and Research Plan for Lakes Mead and Mohave, has …

Effects Of Climate Change On Spring Ecosystem Hydroecology As A Guide To Developing Alternative Water Policies, Scott Mensing, Saxon E. Sharpe, Scott Bassett, Don Sada, Jim Thomas

Climate Change Seminar Series (NNE)

Hydroecology: the interface of ecological systems and water which combines the scientific disciplines of hydrology and ecology

Goal: evaluate the hydrologic and climate history using pollen, loss on ignition, total inorganic carbon, and invertebrates from spring sediments in Spring Valley, Eastern Nevada and Snake Valley, Western Utah

Limnological Assistance For Lake Mead National Recreation Area: Quarterly Report, Period Ending September 30, 2011, Margaret N. Rees

Limnological Studies

Project 1

• Technical assistance with the implementation of the Interagency Monitoring Action Plan (I-MAP) for Quagga Mussels is ongoing. During this quarter, processing of all 2010 juvenile/adult samples was completed; remaining analyses are underway. The project team assisted NPS with 2011 collections.
• A final report on Veliger abundance and settlement at different depths of Lake Mead is in preparation for delivery at the end of November 2011; sample analysis continues for Growth of adult quagga mussels within Las Vegas Wash/Las Vegas Bay with report delivery planned for the end of November 2011.
• The Interagency Quagga Mussel Meeting was held on …

Limnological Assistance For Lake Mead National Recreation Area: Quarterly Report, Period Ending June 30, 2011, Margaret N. Rees

Limnological Studies

Project 1

• Technical assistance with the implementation of the Interagency Monitoring Action Plan (I-MAP) for Quagga Mussels is ongoing. During this quarter, analysis was completed for 42 juvenile/adult samples and analyses of the remaining samples are underway.
• As of 7/07/11 all sample sets will have been collected for Veliger abundance and settlement at different depths of Lake Mead; sampling continues for Growth of adult quagga mussels within Las Vegas Wash/Las Vegas Bay.
• The Interagency Quagga Mussel Meeting was held on 05/17/11; the next meeting is scheduled for 08/25/11.

Project 2

• Appendix 7, an addition to the Long-term Limnological and Aquatic …

Limnological Assistance For Lake Mead National Recreation Area: Quarterly Report, Period Ending March 30, 2011, Margaret N. Rees

Limnological Studies

Project 1

• Technical assistance with the implementation of the Interagency Monitoring Action Plan (I-MAP) for Quagga Mussels is ongoing. During this quarter, analysis was completed for 13 juvenile/adult samples and analyses of the remaining samples are underway.
• Both research projects are well underway with 14 sample sets collected for Veliger abundance and settlement at different depths of Lake Mead and 12 samples collected for Growth of adult quagga mussels within Las Vegas Wash/Las Vegas Bay.

Project 2

• Annual summary sheets are under development for the topics listed within the Limnological and Aquatic Resource Monitoring and Research Plan for Lakes Mead …

Limnological Assistance For Lake Mead National Recreation Area: Quarterly Report, Period Ending January 31, 2011, Margaret N. Rees

Limnological Studies

Project 1

• Technical assistance with the implementation of the Interagency Monitoring Action Plan (I-MAP) for Quagga Mussels is ongoing. During this quarter, analysis was completed for 21 adult/juvenile samples and analyses of the remaining samples are underway. A manuscript describing the I-MAP and its history, current recommended monitoring, and recommended future monitoring and a separate manuscript describing the I-MAP’s monitoring protocol were accepted for publication in the journal Aquatic Invasions. Two other manuscripts describing other aspects of the I-MAP program were published in Lake and Reservoir Management.

Project 2

• Annual summary sheets are under development for the topics listed within …

Abundance And Size Of Quagga Mussels (Dreissena Bugensis) Veligers In Lake Mead, Nevada-Arizona, Shawn Gerstenberger, Sara Ann Mueting, Wai Hing Wong

Environmental & Occupational Health Faculty Publications

The planktonic veligers of the invasive quagga mussel were present year-round from April 2008 to March 2009 in Lake Mead, with high abundance from September to October (>20 veligers/L), whereas the percentage of competent veligers, in terms of the ability to settle, peaked from November 2008 to January 2009 (>60%). The results from this experiment are useful in understanding the life history and population dynamics of quagga mussels in the lower Colorado River Basin.

Quagga Mussels In The Western United States: Monitoring And Management, Wai Hing Wong, Shawn Gerstenberger

Environmental & Occupational Health Faculty Publications

Dreissenid mussels including zebra mussel (Dreissena polymorpha Pallas, 1771), originating from the Ponto-Caspian area, and quagga mussel (Dreissena rostriformis bugensis Andrusov, 1897), originating from the mouths of the Rivers Southern Bug and Dnieper are both species native to Eastern Europe, which were accidently introduced into the Laurentian Great Lakes in North America in the 1980s in ballast water (Carlton 2008; Van der Velde et al 2010). Dreissenid mussels have created severe ecological, recreational and economic impacts on many systems because they are biofoulers and efficient ecological engineers that filter large quantities of water. Examples of these wide-ranging impacts are discussed …

The 100th Meridian Initiative At The Lake Mead National Recreation Area, Nv, Usa: Differences Between Boater Behaviors Before And After A Quagga Mussel, Driessena Rostiformis Bugensis, Invasion, Sara Ann Mueting, Shawn Gerstenberger

Environmental & Occupational Health Faculty Publications

The 100th Meridian Initiative was developed to help prevent the spread of aquatic nuisance species (ANS) through boater education and research on boater movement patterns and behaviors. Surveys employing these elements were conducted at Lake Mead National Recreation Area (LMNRA) in 2002-2003 before the discovery of the quagga mussel (Dreissena rostiformis bugensis Andrusov 1897) and in 2007-2008 after an established population of quagga mussels was found in the Lake. Boaters were asked questions in a personal interview or a mail-in survey regarding what body of water they had previously launched their watercraft in, where they were planning to launch next, …

Interagency Monitoring Action Plan (I-Map) For Quagga Mussels In Lake Mead, Nevada-Arizona, Usa, Kent Turner, Wai Hing Wong, Shawn Gerstenberger, Jennell M. Miller

Environmental & Occupational Health Faculty Publications

Following the discovery of quagga mussels in Lake Mead, Nevada-Arizona, USA, a variety of federal, state and regional agencies set up monitoring programs to evaluate and gain information to help minimize the impacts, or potential impacts, of quagga mussels to their facilities and lake ecology. While the agencies have worked closely and shared monitoring data and findings from the beginning of the infestation, there has been no documented comprehensive monitoring program to describe and record the various quagga mussel-related monitoring needs. Ad hoc interagency quagga mussel meeting representatives established an Interagency Monitoring Action Plan (I-MAP), which outlines agency objectives related …

Environmental Factors Affecting Settlement Of Quagga Mussel (Dreissena Rostriformis Bugensis) Veligers In Lake Mead, Nevada-Arizona, Usa, Dong Chen, Shawn Gerstenberger, Sara Ann Mueting, Wai Hing Wong

Environmental & Occupational Health Faculty Publications

Environmental factors that can affect the settlement rate of quagga mussel veligers include flow velocity, water temperature, pH, dissolved oxygen (DO), conductivity, total organic carbon (TOC), and the surface roughness of monitoring substrates. In the present study, six artificial substrates, Acrylonitrile Butadiene Styrene (ABS) plastic, High Density Polyethylene (HDPE) plastic, Concrete Underlayment Board (CUB), aluminum, stainless steel and fiberglass, were used to monitor the settlement of quagga mussel veligers at different water depths in Lake Mead, Nevada-Arizona, USA. Considering the hierarchical data structure of observed mussel densities, we investigated the relationship between mussel settlement on monitoring substrates and the surrounding …

A Standardized Design For Quagga Mussel Monitoring In Lake Mead, Nevada-Arizona, Wai Hing Wong, Shawn Gerstenberger, Jennell M. Miller, Craig Palmer, Bryan Moore

Environmental & Occupational Health Faculty Publications

The discovery of quagga mussels (Dreissena rostriformis bugensis) in Lake Mead, Nevada-Arizona, on January 6, 2007 is the first known occurrence of dreissenid species in the western United States. This study developed elements of a cost-effective and standardized quagga mussel-monitoring program for Lake Mead using preliminary data to arrive at statistically based numbers of sampling sites. To represent the abundance of adult/juvenile quagga mussels in Lake Mead’s heterogeneous floor with 95% confidence, a stratified simple random sampling design revealed a requirement of 41 samples from hard substrates (i.e., rocky areas) and 97 samples from soft substrates (i.e., sandy and muddy …

Limnological Assistance For Lake Mead National Recreation Area: Quarterly Report, Period Ending September 30, 2010, Margaret N. Rees

Limnological Studies

Project 1

• Technical assistance with the implementation of the Interagency Monitoring Action Plan (I-MAP) for Quagga Mussels is ongoing. During this quarter, analysis was completed for 11 adult/juvenile samples from the winter 2009 – spring 2010 sampling. Two manuscripts related to the I-MAP were submitted to the peer-reviewed journal, Aquatic Invasions.

Project 2

• Topics and analyses have been approved for annual report development as prescribed by the Limnological and Aquatic Resource Monitoring and Research Plan for Lakes Mead and Mohave.
• Content, including links and photos for the front page, four limnology category web pages, and one associated page have been …

Limnological Assistance For Lake Mead National Recreation Area: Quarterly Report, Period Ending June 30, 2010, Margaret N. Rees

Limnological Studies

Project 1

• Technical assistance with the implementation of the Interagency Monitoring Action Plan (I-MAP) for Quagga Mussels is ongoing. During this quarter, analysis was completed for 14 adult/juvenile samples from the fall 2009 – spring 2010 sampling.
• Sampling for the first of two small-scale research projects funded by this task agreement (Abundance and settlement at different depths of Lake Mead) was initiated on June 16, 2010.

Project 2

• Topics and proposed analyses have been drafted for annual report development as prescribed by the Plan.
• A plan for developing and organizing NPS limnology-related web pages has been documented and content has …

Limnological Assistance For Lake Mead National Recreation Area: Quarterly Report, Period Ending March 30, 2010, Margaret N. Rees

Limnological Studies

Project 1

• Technical assistance with the implementation of the Interagency Monitoring Action Plan (I-MAP) for Quagga Mussels is ongoing. Three sampling events took place this quarter and analysis of all samples (fall 2009 – spring 2010) is underway.

• Two graduate students are undergoing necessary training for the two small-scale research projects funded by this task agreement. Sampling is anticipated to begin in June 2010.

• The quarterly Interagency Quagga Mussel Meeting was held in March 2010.

Project 2

• Protocols relevant to the Long-term Limnological Aquatic Resource Monitoring and Research Plan (Plan) for Lakes Mead and Mohave have …

Limnological Assistance For The Lake Mead National Recreation Area In Meeting The Challenge Of The Water 2025 Initiative: Executive Summary And Concise Statement Of Accomplishments, Limnological Assistance For Lake Mead National Recreation Area

Limnological Studies

Limnological Assistance for the Lake Mead National Recreation Area in Meeting the Challenge of the Water 2025 Initiative was a Southern Nevada Public Land Management Act (SNPLMA)-funded project. It was implemented by the University of Nevada, Las Vegas (UNLV) Public Lands Institute (PLI) cooperatively with the UNLV Department of Environmental and Occupational Health and the UNLV Harry Reid Center for Environmental Studies on behalf of and in cooperation with the National Park Service, Lake Mead National Recreation Area (Lake Mead NRA).

Joint Fire Science Program – Lake Mead National Recreation Area Revegetating Burned Arid Lands: Identifying Successful Native Species Using Trait And Competition Analysis: Quarterly Progress Report, Time Period: July 1 — September 30, 2009, Margaret N. Rees

Fire Science

• Weighed biomass for competitive hierarchy study.
• Completed more seed granivory trials at Goodsprings.
• Monitored and maintained nursery plots.
• Submitted revised article to Journal of Arid Environments.

Limnological Assistance For The Lake Mead National Recreation Area In Meeting The Challenge Of The Water 2025 Initiative: Quarterly Report, Period Ending September 30, 2009, Margaret N. Rees

Limnological Studies

Project 1: Two advisory team meetings were attended this year; technical input was provided as appropriate; and summaries were prepared and delivered to Kent Turner. The SCOP Selenium Management Plan has been reviewed with two reports provided.

Project 2: Whitepapers on the carp die-off due to Koi Herpes Virus (KHV) and on lead-base paint use on launch ramps were finalized and delivered to Mr. Turner.

Project 3: The Interagency Monitoring Action Plan (I-MAP) for Quagga Mussels has been approved for implementation.

Project 5: Seven articles based on presentations at the Lake Mead Science Symposium have been approved by the technical …

Interagency Management Action Plan (I-Map) For Quagga Mussels, David Wong, Shawn Gerstenberger

Public Lands Institute Publications

Following the discovery of quagga mussels in Lake Mead, a variety of agencies, including National Park Service (NPS) Lake Mead National Recreational Area (LMNRA), Bureau of Reclamation (USBR), Southern Nevada Water Authority (SNWA), Nevada Department of Wildlife (NDOW), U.S. Fish and Wildlife (USFWS), Clean Water Coalition (CWC), and U.S. Geological Survey (USGS) have set up monitoring programs to evaluate and gain information to help minimize the impacts or potential impacts of quagga mussels to their facilities and lake ecology. Current monitoring activities and anticipated environmental impacts are depicted in Figures 1 and 2. While the agencies have worked closely and …

Limnological Assistance For The Lake Mead National Recreation Area In Meeting The Challenge Of The Water 2025 Initiative: Annual Report, Period Ending July 1, 2009, Margaret N. Rees

Limnological Studies

Project 1: Eighteen advisory team meetings were attended this year; technical input was provided as appropriate; and summaries were prepared and delivered to Kent Turner. The SCOP Selenium Management Plan has been reviewed and a report is in preparation.

Project 2: A formal report titled, Surface Water Monitoring for Indicator Bacteria in High-use Sites of the Lake Mead National Recreation Area, was delivered in the second quarter of this year. Subsequently, a poster presentation on this topic was delivered at the Lake Mead Science Symposium and a manuscript has been prepared for submission to the journal, Lake and Reservoir Management. …

Joint Fire Science Program – Lake Mead National Recreation Area: Revegetating Burned Arid Lands: Identifying Successful Native Species Using Trait And Competition Analysis, Quarterly Progress Report, April 1 — June 30, 2009, Margaret N. Rees

Fire Science

• Initiated a competitive hierarchy study.
• Monitored and maintained outplanting at Goodsprings.
• Completed more seed granivory trials at Goodsprings.
• Mentored student to completion of undergraduate research project.
• Monitored and maintained nursery plots.
• Revising article submitted to Journal of Arid Environments.

Limnological Assistance For The Lake Mead National Recreation Area In Meeting The Challenge Of The Water 2025 Initiative: Quarterly Report, Period Ending April 1, 2009, Margaret N. Rees

Limnological Studies

Project 1: Technical input has been provided at two advisory team meetings attended this quarter.

Project 2: Surface Water Monitoring for Indicator Bacteria in High-use Sites of the Lake Mead National Recreation Area (poster) was presented at the Lake Mead Science Symposium; a journal article is in preparation.

Project 3: A draft document titled “Interagency Monitoring Action Plan (I-MAP): Quagga Mussels in Lakes Mead and Mohave” has been subdivided into four major sections and corresponding sub-groups have been formed to provide review and input. Six oral presentations related to quagga mussels were presented at the Lake Mead Science Symposium. Two …

Joint Fire Science Program – Lake Mead National Recreation Area: Revegetating Burned Arid Lands: Identifying Successful Native Species Using Trait And Competition Analysis, Quarterly Progress Report, January 1— March 31, 2009, Margaret N. Rees

Fire Science

• Monitored and watered native plants in nursery plots.

• Exotic grasses seeded into nursery plots.

• Nitrogen treatment applied to nursery plots.

• Granivory study trials performed each month and corresponding abstract submitted to ESA.

• Competitive hierarchy study germination trials are underway.

• Monitored seeding and outplanting experiments at Goodsprings.

• Submitted article to Mojave Applied Ecology Notes newsletter updating on JFS work.

• Mentoring UNLV undergraduate student in research project for ENV 492.

• Prepared presentation materials for two scientific meetings.

Joint Fire Science Program – Lake Mead National Recreation Area: Revegetating Burned Arid Lands: Identifying Successful Native Species Using Trait And Competition Analysis, Quarterly Progress Report, October 1 — December 31, 2008, Margaret N. Rees

Fire Science

• Presented poster at Natural Areas Conference in Nashville, TN

• Initiated granivory study at Goodsprings.

• Outplanted 2376 plants for Experiment 1 at Lake Mead nursery.

• Presented poster at Wildfires and Invasive Plants in American Deserts conference in Reno, NV.

• Submitted manuscript covering our distance from road - microsite invasibility study to Journal of Arid Environments.