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Terrestrial and Aquatic Ecology

Eastern Kentucky University

Conservation

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Full-Text Articles in Life Sciences

Are Mussel Shells Environmental Dna Time Capsules? A Comparison Of Extraction Methods For Obtaining Dna From Shell Material., Kelsey Elizabeth Rogers Jan 2017

Are Mussel Shells Environmental Dna Time Capsules? A Comparison Of Extraction Methods For Obtaining Dna From Shell Material., Kelsey Elizabeth Rogers

Online Theses and Dissertations

Freshwater mussels have become some of the most imperiled species in North America and widespread populations have succumbed to pollution and many other anthropogenic-related factors. With molecular techniques evolving, a recent interest in ancient DNA and museum specimens has emerged and prompted a study to test the ability of several extraction methods to isolate DNA from museum mussel specimens. The purpose of this study was to determine if four DNA extraction methods had influence on total DNA yield (ng/mg) from mussel tissue. The hinge ligaments of freshwater mussels ranging in collection date (1984-2015) were used as the source of genetic …