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High-Frequency Electrically Stimulated Skeletal Muscle Contractions Increase P70S6k Phosphorylation Independent Of Known Igf-I Sensitive Signaling Pathways, Sarah Witkowski, Richard M. Lovering, Espen E. Spangenburg

Exercise and Sport Studies: Faculty Publications

Insulin-like growth factor (IGF-I) is hypothesized to be a critical upstream regulator of mammalian target of rapamycin (mTOR)-regulated protein synthesis with muscle contraction. We utilized a mouse model that expresses a skeletal muscle specific dominant-negative IGF-I receptor to investigate the role of IGF-I signaling of protein synthesis in response to unilateral lengthening contractions (10 sets, 6 repetitions, 100. Hz) at 0 and 3. h following the stimulus. Our results indicate that one session of high frequency muscle contractions can activate mTOR signaling independent of signaling components directly downstream of the receptor.