Life Sciences Commons^™

Denial Of Pregnancy Or Cryptic Pregnancy, Nadia N. Shuriah, Elaine Mcfarland, Bethany Spenn, Kaisha Butz Mcmanus

BIO4960: Practicum in Biology I

Denial of pregnancy, or cryptic pregnancy, presents a complex situation where individuals are unaware of their pregnancy until the late stages of labor. This study delves into the multifaceted nature of concealed and denied pregnancies, exploring their prevalence, factors contributing to them, and their implications for both individuals and healthcare systems. By reviewing relevant literature and case studies, we aim to illuminate the difficulties healthcare providers face in identifying and managing cases of denial of pregnancy. Additionally, we stress the significance of proactive screening and comprehensive care to ensure the well-being of mothers and infants. This research endeavors to deepen …

Understanding The Microbial Biogeography Of Ancient Human Dentitions To Guide Study Design And Interpretation, Zandra Fagernäs, Domingo C. Salazar-Garcia, Maria Hadar Uriarte, Azucena Avilés Fernández, Amanda G. Henry, Joaquín Lomba Maurandi, Andrew T. Ozga, Irina M. Velsko, Christina Warinner

Biology Faculty Articles

The oral cavity is a heterogeneous environment, varying in factors such as pH, oxygen levels, and salivary flow. These factors affect the microbial community composition and distribution of species in dental plaque, but it is not known how well these patterns are reflected in archaeological dental calculus. In most archaeological studies, a single sample of dental calculus is studied per individual and is assumed to represent the entire oral cavity. However, it is not known if this sampling strategy introduces biases into studies of the ancient oral microbiome. Here, we present the results of a shotgun metagenomic study of a …

Medical & Psychological Clinical Case Competition, Halmos College Of Arts And Sciences (Hcas)

HCAS Case Competitions

No abstract provided.

Using Ecological Analyses To Characterize Socio-Economic Food Deserts, Annie Goyanes, J. Matthew Hoch

Marine & Environmental Sciences Faculty Datasets

“Food desert” refers to an area where citizens lack access to fresh, affordable, nutritious food. Food deserts are generally in low-income urban neighborhoods or rural towns and can be considered environmental or economic injustice. South Florida and Broward County have been documented as having a high food insecurity rate, indicative of households having difficulty putting food on the table. Given the high rate of food insecurity, it was hypothesized that a lack of access to food and the presence of socio-economic food deserts was at least partially responsible. Data was collected by visiting three supermarkets in each of the 13 …

"The Dead Shall Be Raised": Multidisciplinary Analysis Of Human Skeletons Reveals Complexity In 19th Century Immigrant Socioeconomic History And Identity In New Haven, Connecticut, Gary P. Aronsen, Lars Fehren-Schmitz, John Krigbaum, George D. Kamenov, Gerald J. Conlogue, Christina Warinner, Andrew T. Ozga, Krithivasan Sankaranarayanan, Anthony Griego, Daniel W. Deluca, Howard T. Eckels, Romuald K. Byczkiewicz, Tania Grgurich, Natalie A. Pelletier, Sarah A. Brownlee, Ana Marichal, Kylie Williamson, Yukiko Tonoike, Nicholas F. Bellantoni

Biology Faculty Articles

In July 2011, renovations to Yale-New Haven Hospital inadvertently exposed the cemetery of Christ Church, New Haven, Connecticut’s first Catholic cemetery. While this cemetery was active between 1833 and 1851, both the church and its cemetery disappeared from public records, making the discovery serendipitous. Four relatively well-preserved adult skeletons were recovered with few artifacts. All four individuals show indicators of manual labor, health and disease stressors, and dental health issues. Two show indicators of trauma, with the possibility of judicial hanging in one individual. Musculoskeletal markings are consistent with physical stress, and two individuals have arthritic indicators of repetitive movement/specialized …

Comparison Of Two Ancient Dna Extraction Protocols For Skeletal Remains From Tropical Environments, Maria A. Nieves-Colon, Andrew T. Ozga, William J. Pestle, Andrea Cucina, Vera Tiesler, Travis W. Stanton, Anne C. Stone

Biology Faculty Articles

Objectives

The tropics harbor a large part of the world's biodiversity and have a long history of human habitation. However, paleogenomics research in these climates has been constrained so far by poor ancient DNA yields. Here we compare the performance of two DNA extraction methods on ancient samples of teeth and petrous portions excavated from tropical and semi‐tropical sites in Tanzania, Mexico, and Puerto Rico (N = 12).

Materials and Methods

All samples were extracted twice, built into double‐stranded sequencing libraries, and shotgun sequenced on the Illumina HiSeq 2500. The first extraction protocol, Method D, was previously designed for …

Differential Preservation Of Endogenous Human And Microbial Dna In Dental Calculus And Dentin, Allison E. Mann, Susanna Sabin, Kirsten Ziesemer, Ashild J. Vagene, Hannes Schroeder, Andrew T. Ozga, Krithivasan Sankaranarayanan, Courtney A. Hofman, James A. Fellows Yates, Domingo C. Salazar-Garcia, Bruno Frohlich, Mark Aldenderfer, Menno Hoogland, Christopher Read, George R. Milner, Anne C. Stone, Cecil M. Lewis Jr., Johannes Krause, Corinne Hofman, Kirsten I. Bos, Christina Warinner

Biology Faculty Articles

Dental calculus (calcified dental plaque) is prevalent in archaeological skeletal collections and is a rich source of oral microbiome and host-derived ancient biomolecules. Recently, it has been proposed that dental calculus may provide a more robust environment for DNA preservation than other skeletal remains, but this has not been systematically tested. In this study, shotgun-sequenced data from paired dental calculus and dentin samples from 48 globally distributed individuals are compared using a metagenomic approach. Overall, we find DNA from dental calculus is consistently more abundant and less contaminated than DNA from dentin. The majority of DNA in dental calculus is …

Hypocrisy And Corruption: How Disparities In Power Shape The Evolution Of Social Control, Omar T. Eldakar, J. Oliver Kammeyer, Nikhil Nagabandi, Andrew C. Gallup

Biology Faculty Articles

Altruism presents an evolutionary paradox, as altruistic individuals are good for the group yet vulnerable to exploitation by selfish individuals. One mechanism that can effectively curtail selfishness within groups is punishment. Here, we show in an evolutionary game-theoretical model that punishment can effectively evolve and maintain high levels of altruism in the population, yet not all punishment strategies were equally virtuous. Unlike typical models of social evolution, we explicitly altered the extent to which individuals vary in their power over others, such that powerful individuals can more readily punish and escape the punishment of others. Two primary findings emerged. Under …

A Novel On‐Chip Method For Differential Extraction Of Sperm In Forensic Cases, Fatih Inci, Mehmet O. Ozen, Yeseren Saylan, Morteza Miansari, Duygu Cimen, Raghu Dhara, Thiruppathiraja Chinnasamy, Mehmet Yuksekkaya, Chiara Filippini, Deepan Kishore Kumar, Semih Calamak, Yusuf Yesil, Naside Gozde Durmus, George Duncan, Leonard Klevan, Utkan Demirci

Biology Faculty Articles

One out of every six American women has been the victim of a sexual assault in their lifetime. However, the DNA casework backlog continues to increase outpacing the nation's capacity since DNA evidence processing in sexual assault casework remains a bottleneck due to laborious and time‐consuming differential extraction of victim's and perpetrator's cells. Additionally, a significant amount (60–90%) of male DNA evidence may be lost with existing procedures. Here, a microfluidic method is developed that selectively captures sperm using a unique oligosaccharide sequence (Sialyl‐LewisX), a major carbohydrate ligand for sperm‐egg binding. This method is validated with forensic mock samples dating …

Successful Enrichment And Recovery Of Whole Mitochondrial Genomes From Ancient Human Dental Calculus, Andrew T. Ozga, Maria A. Nieves-Colon, Tanvi P. Honap, Krithivasan Sankaranarayanan, Courtney A. Hofman, George R. Milner, Cecil M. Lewis Jr., Anne C. Stone, Christina Warinner

Biology Faculty Articles

Objectives

Archaeological dental calculus is a rich source of host‐associated biomolecules. Importantly, however, dental calculus is more accurately described as a calcified microbial biofilm than a host tissue. As such, concerns regarding destructive analysis of human remains may not apply as strongly to dental calculus, opening the possibility of obtaining human health and ancestry information from dental calculus in cases where destructive analysis of conventional skeletal remains is not permitted. Here we investigate the preservation of human mitochondrial DNA (mtDNA) in archaeological dental calculus and its potential for full mitochondrial genome (mitogenome) reconstruction in maternal lineage ancestry analysis.

Materials and …

Origins Of An Unmarked Georgia Cemetery Using Ancient Dna Analysis, Andrew T. Ozga, Raul Y. Tito, Brian M. Kemp, Hugh Matternes, Alexandra J. Obregon-Tito, Leslie Neal, Cecil M. Lewis Jr.

Biology Faculty Articles

Determining the origins of those buried within undocumented cemeteries is of incredible importance to historical archaeologists and, in many cases, the nearby communities. In the case of Avondale Burial Place, a cemetery in Bibb County, Georgia, in use from 1820 to 1950, all written documentation of those interred within it has been lost. Osteological and archaeological evidence alone could not describe, with confidence, the ancestral origins of the 101 individuals buried there. In the present study, we used ancient DNA extraction methods in well-preserved skeletal fragments from 20 individuals buried in Avondale Burial Place to investigate the origins of the …

Validation Of A Short Tandem Repeat Multiplex Typing System For Genetic Individualization Of Domestic Cat Samples, Nikia Coomber, Victor A. David, Stephen J. O'Brien, Marilyn Menotti-Raymond

Biology Faculty Articles

Aim To conduct developmental validation studies on a polymerase chain reaction (PCR) based short tandem repeat (STR) multiplex typing system, developed for the purpose of genetic individualization and parentage testing in domestic cat samples.

Methods To evaluate reproducibility of the typing system, the multiplex was amplified using DNA extracted from hair, blood, and buccal samples obtained from the same individual (n = 13). Additional studies were performed to evaluate the system’s species’ specificity, using 26 North American mammalian species and two prokaryotes Sacchromyces and Escherichia coli, sensitivity, and ability to identify DNA mixtures. Patterns of Mendelian inheritance and mutation …

Creating And Presenting A Poster At The Undergraduate Student Symposium, Beatrix Aukszi, Christopher A. Blanar, Weylin Sternglanz

Chemistry and Physics Faculty Proceedings, Presentations, Speeches, Lectures

No abstract provided.