Open Access. Powered by Scholars. Published by Universities.®
- Institution
- Publication
- Publication Type
Articles 1 - 4 of 4
Full-Text Articles in Life Sciences
Sex Differences In Stress Reactivity, Brain Morphology, And Oxytocin In The Hypothalamus Of The Gray Short-Tailed Opossum (Monodelphis Domestica), Esperanza I. Zacarias, Daniela Rodriguez, Alexandra Chalons, Sasawan Heingraj, Nicole Altamirano, Joseph Rafac, John L. Vandeberg, Mario Gil
Sex Differences In Stress Reactivity, Brain Morphology, And Oxytocin In The Hypothalamus Of The Gray Short-Tailed Opossum (Monodelphis Domestica), Esperanza I. Zacarias, Daniela Rodriguez, Alexandra Chalons, Sasawan Heingraj, Nicole Altamirano, Joseph Rafac, John L. Vandeberg, Mario Gil
Research Colloquium
Understanding the effects of stress on behavior and cognition is important due to its impact on mental health and wellbeing (Schneiderman et al. 2005). Translational animal research can contribute to the development of new treatments that can improve therapeutic outcomes and our understanding of the neurobiology of stress. In the present study, we complement behavioral stress reactivity with immunohistochemical localization of oxytocin in the hypothalamus, a neuropeptide that regulates stress (Neumann & Slattery, 2016). Oxytocin has potential therapeutic use for mental health disorders (Neumann & Slattery, 2016), and the effects of oxytocin seem to be sexually dimorphic (Love, 2018). Using …
Characterizing Rat Lateral Amygdala Interneurons By Colocalization Of Calcium-Binding Proteins Via Serial Multiplex Immunohistochemistry, Ethan Gasteyer
Characterizing Rat Lateral Amygdala Interneurons By Colocalization Of Calcium-Binding Proteins Via Serial Multiplex Immunohistochemistry, Ethan Gasteyer
Honors Scholar Theses
The lateral amygdala is a brain structure that plays an important role in regulating fear and anxiety. Some anxiety disorders are hypothesized to develop from failures in this local inhibitory circuit. Distinct populations of these inhibitory neurons express patterns of calcium- binding proteins and neuropeptides that suggest differences in functionality within the lateral amygdala. Furthermore, these patterns of expression are compared between male and female rats to identify sex differences in the lateral amygdala. Previous studies have reported sex differences in amygdala activation and connectivity, but very little is known about the sexual dimorphism of calcium-binding protein and neuropeptide expression. …
Microglia-Neuron Interactions In A Mouse Model Of Low Grade Neuroepithelial Tumors, Veolette Hanna
Microglia-Neuron Interactions In A Mouse Model Of Low Grade Neuroepithelial Tumors, Veolette Hanna
Honors Scholar Theses
Microglia are the macrophages of the brain and spinal cord, playing an important role in the immune response to disease states of the nervous system. This study conducts an investigation on the activity of microglia in response to low grade neuroepithelial tumors. Using mouse models and microglial markers, a qualitative and quantitative analysis of microglia activation, migration, and invasion within the brain cortex during early stages of tumor development was conducted. It was found that the presence of a low grade neuroepithelial tumor in the cortex of one hemisphere of the brain causes significant microglia activation in comparison to the …
Optimization Of Primary Cilia Detection In Musculoskeletal Tissue, Ragen Engel
Optimization Of Primary Cilia Detection In Musculoskeletal Tissue, Ragen Engel
Williams Honors College, Honors Research Projects
The objective of this research project was to optimize a laboratory technique for visualizing primary cilia in musculoskeletal tissue of young swine, specifically the growth plate of long bones. This was accomplished through varying fixation procedures, antigen retrieval methods, antibody concentrations, and incubation times. By varying these parameters, a reproducible procedure was developed to examine the primary cilia in multiple tissues including ligament, fascia, and growth plate. This experiment introduced variances in fixation methods, with methanol or formalin. Paraffin and frozen embedding techniques were also varied for comparison with regards to cilia visualization. Results from the acetylated alpha tubulin (aTUB) …