Life Sciences Commons^™

Effect Of Radiation And Corynebacterium Parvum On The In Vivo Elimination Of Friend Virus-Induced Leukemia In C57bl/6 Mice, Betty A. Wu

Loma Linda University Electronic Theses, Dissertations & Projects

Administration of Corynebacterium parvum in combination with localized irradiation has been shown to be more efficient than radiation treatment alone in prolonging the survival time of methylethrane induced tumor in C3H mice. Fractionated low dose total body irradiation has been demonstrated to be a promising therapy for patients with chronic lymphocytic leukemia. The purpose of this study was to determine the effect of the combined treatment of total body irradiation and C. parvum on Friend virus induced leukemia in C57BL/6 mice. ^₁₂₅I-labeled FBL-3 cells were injected intraperitoneally into mice. Intraperitoneal injection of C. parvum and and single dose total …

The Effects Of Inducing Functional Class Iii Malocclusion On Actively Growing Rat Mandibles, Aarin Chu

Loma Linda University Electronic Theses, Dissertations & Projects

[Abstract Not Included]

The Effect Of Endodontic Therapy On The Incidence Of Radiation Caries In Dogs, James A. Abbott

Loma Linda University Electronic Theses, Dissertations & Projects

Endodontic therapy was evaluated as a possible control or prevention modality of radiation type dental caries in dogs. Two adult male dogs with permanent dentitions had endodontic therapy performed on alternating teeth in each jaw. The nontreated teeth served as controls.

The dogs were irradiated with 7000 rad Cobalt-60 radiation fractionated over an eight week period. The dogs were killed at 60 and 182 days post-irradiation. The teeth were removed from the jaws and immersed in methylene blue dye, sectioned, and the dye penetration pattern photographed. Diffusion of the dye into the tooth structure was quantified and statistically analyzed.

A …

Human Placental Alkaline Phosphatase And Steroid Metabolism By Rat Adrenals, Allen G. Meier

Loma Linda University Electronic Theses, Dissertations & Projects

Human placental alkaline phosphatase was purified by butanol extraction, methanol precipitation and chromatography on DEAE-cellulose. The pH optimum was found to be 10.9. The enzyme was competitively inhibited by di-ethyl-p-nitrobenzyl-phosphonate and copper sulfate. An increase in enzymatic activity occurred in the presence of the phosphate ion (HPO₄) and ATP.

The enzyme was crystallized from a 40% saturated solution of ammonium sulfate and shown to be homogeneous by sedimentation analysis. Homogeneity of the enzyme was also demonstrated using poly-acrylamide-gel-electrophoresis. At a pH of 7.5% the electrophoretic pattern yielded a single band and showed no significant contamination.

The metabolic transformation …

Apical Closure Induction Using Bone Growth Factors And Mineral Trioxide Aggregate, Kenneth W. Tittle

Loma Linda University Electronic Theses, Dissertations & Projects

This study compared the ability of three different bone growth factor combinations with that of Ca(OH)₂ to stimulate apical closure as well as the effectiveness of Mineral Trioxide Aggregate (MTA) as an apical barrier. Fifty-six roots of second and fourth mandibular premolars of seven six- month old beagle dogs were used. Periradicular lesions were created over a four week period. The canals were cleaned and shaped and filled with Ca(OH)₂ for one week. They were then irrigated and received one of seven different treatments in a balanced design. Growth factors were placed in a carboxymethylcellulose carrier and delivered …