Life Sciences Commons^™

Characterization And Functional Rescue Of Congenital Muscular Dystrophy With Megaconial Myopathy In A Mouse Model Of The Disease, Ambreen A. Sayed

Electronic Theses and Dissertations

Congenital muscular dystrophy with megaconial myopathy (MDCMC) is an autosomal recessive disorder characterized by progressive muscle weakness and wasting. Megamitochondria in skeletal muscle biopsies and cognitive impairments in MDCMC patients are observations exclusive to this type of muscular dystrophy. The disease is caused by loss of function mutations in the choline kinase beta (CHKB) gene which results in dysfunction of the Kennedy pathway for the synthesis of phosphatidylcholine (PC). A rostro-caudal muscular dystrophy (rmd) mouse with a deletion in the Chkb gene resulting in MDCMC-like symptoms has been reported by our lab. In order to test if the rmd mice …

Assessing The Upper Critical Limit Of The Thermoneutral Zone In Laboratory Mice, Teumbo Ngunte

Electronic Theses and Dissertations

Endothermic organisms such as mammals and birds rely on high energy budgets to regulate body temperature. Many studies have previously investigated the thermo-regulation of mammals under the lower critical temperature of the thermoneutral zone, yet our knowledge in determining the upper critical limits is still scarce. As an endotherm, lab mice (Mus musculus) are perfect models to determine the cost to maintain constant body temperature as ambient temperature increases. The upper critical temperature of the thermoneutral zone of this species has been estimated to be above 32°C and below 34°C. By utilizing different genetic backgrounds of lab mice in this …

Providing Experimental Evidence For Mouse Telomerase Reverse Transcriptase As A Novel And Unique Adult Neural Stem Cell Marker, Caroline Dean Curtis

Electronic Theses and Dissertations

With the prevalence of neurodegenerative pathologies in our society today it is imperative that we begin to look at novel approaches to the underlying problem of dying neurons that are not replaced. Adult neural stem cells exist naturally and could potentially be manipulated into targeted repair of damaged brains, given substantial research. The first step in this process is to find a way to specifically mark the earliest subset of these cells, the quiescent adult neural stem cells. Here we provide evidence for the existence of a novel and unique qANSC marker in mouse telomerase reverse transcriptase (mTERT). mTERT has …

Modeling Human Cancer Therapy Response In Patient Derived Xenografts, Joan Malcolm

Electronic Theses and Dissertations

Patient‐derived xenografts (PDXs) generated by implanting human tumor tissue into a transplant compliant mouse host have been of increasingly importance to preclinical development and have been demonstrated to have advantages compared to cancer cell lines and cell‐line xenografts (CLX) for modeling therapeutic responses in cancer. Nevertheless, many open questions remain regarding the relationship between study design factors and classification of treatment response and the molecular fidelity of tumors passaged in PDXs relative to the original patient tumor(s). The research described in this dissertation addresses both of these significant issues related to the use of PDXs as a tool for modeling …

Wildlife Use Of Vernal Pools In An Urbanizing Landscape With A Focus On Population Vitality Of Vernal Pool-Breeding Amphibians, Carly Eakin

Electronic Theses and Dissertations

Vernal pools in the northeastern United States provide essential habitat for pool-breeding amphibians and provide resources for other forest-dwelling wildlife. These pools and pool-breeding amphibians in particular are threatened by land conversion associated with urbanization and urban-associated factors. The responses of these amphibians and of birds and mammals using vernal pools to intermediate levels of urban development are largely unknown. I used field observations and lab experiments to study the amphibians, birds, and mammals associated with vernal pools along an urban development gradient in greater Bangor, Maine.

In Chapter 1, I examined bird and mammal use and assemblage composition at …