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Full-Text Articles in Life Sciences

Unique Genome-Wide Transcriptome Profiles Of Chicken Macrophages Exposed To Salmonella-Derived Endotoxin, Ceren Ciraci, Christopher K. Tuggle, Michael J. Wannemeuhler, Dan Nettleton, Susan J. Lamont Jul 2019

Unique Genome-Wide Transcriptome Profiles Of Chicken Macrophages Exposed To Salmonella-Derived Endotoxin, Ceren Ciraci, Christopher K. Tuggle, Michael J. Wannemeuhler, Dan Nettleton, Susan J. Lamont

Dan Nettleton

Background: Macrophages play essential roles in both innate and adaptive immune responses. Bacteria require endotoxin, a complex lipopolysaccharide, for outer membrane permeability and the host interprets endotoxin as a signal to initiate an innate immune response. The focus of this study is kinetic and global transcriptional analysis of the chicken macrophage response to in vitro stimulation with endotoxin from Salmonella typhimurium-798.

Results: The 38535-probeset Affymetrix GeneChip Chicken Genome array was used to profile transcriptional response to endotoxin 1, 2, 4, and 8 hours post stimulation (hps). Using a maximum FDR (False Discovery Rate) of 0.05 to declare genes as differentially …


Whole Egg Consumption Impairs Insulin Sensitivity In Rat Model Of Obesity And Type 2 Diabetes, Cassondra J. Saande, Megan A. Steffes, Joseph L. Webb, Rudy J. Valentine, Matthew J. Rowling, Kevin Schalinske Mar 2019

Whole Egg Consumption Impairs Insulin Sensitivity In Rat Model Of Obesity And Type 2 Diabetes, Cassondra J. Saande, Megan A. Steffes, Joseph L. Webb, Rudy J. Valentine, Matthew J. Rowling, Kevin Schalinske

Rudy Valentine

Background: The literature regarding the relation between egg consumption and type 2 diabetes (T2D) is inconsistent and there is limited evidence pertaining to the impact of egg consumption on measures of insulin sensitivity. Objective: The objective of this study was to investigate the effect of dietary whole egg on metabolic biomarkers of insulin resistance in T2D rats. Downloaded from https://academic.oup.com/cdn/advance-article-abstract/doi/10.1093/cdn/nzz015/5374517 by Iowa State University user on 28 March 2019 Methods: Male Zucker diabetic fatty rats (n=12; 6 wk of age) and their lean controls (n=12; 6 wk of age) were randomly assigned to a casein- or whole egg-based diet. At …


Transcriptome Analysis In Spleen Reveals Differential Regulation Of Response To Newcastle Disease Virus In Two Chicken Lines, Jibin Zhang, Michael G. Kaiser, Melissa S. Deist, Rodrigo A. Gallardo, David A. Bunn, Terra R. Kelly, Jack C.M. Dekkers, Huaijun Zhou, Susan J. Lamont Jan 2018

Transcriptome Analysis In Spleen Reveals Differential Regulation Of Response To Newcastle Disease Virus In Two Chicken Lines, Jibin Zhang, Michael G. Kaiser, Melissa S. Deist, Rodrigo A. Gallardo, David A. Bunn, Terra R. Kelly, Jack C.M. Dekkers, Huaijun Zhou, Susan J. Lamont

Jibin Zhang

Enhancing genetic resistance of chickens to Newcastle Disease Virus (NDV) provides a promising way to improve poultry health, and to alleviate poverty and food insecurity in developing countries. In this study, two inbred chicken lines with different responses to NDV, Fayoumi and Leghorn, were challenged with LaSota NDV strain at 21 days of age. Through transcriptome analysis, gene expression in spleen at 2 and 6 days post-inoculation was compared between NDV-infected and control groups, as well as between chicken lines. At a false discovery rate <0.05, Fayoumi chickens, which are relatively more resistant to NDV, showed fewer differentially expressed genes …


Gene Expression Response To Heat Stress In Two Broiler Lines, Jibin Zhang, Carl J. Schmidt, Susan J. Lamont Mar 2016

Gene Expression Response To Heat Stress In Two Broiler Lines, Jibin Zhang, Carl J. Schmidt, Susan J. Lamont

Jibin Zhang

The fast growth rate and increased body weight of modern broilers makes them susceptible to heart failure and sensitive to heat stress. To find the genetic causes of these problems, gene expression was measured in hearts of two broiler lines that differed in their response to heat stress. Each line was separated into two groups that were kept under heat stress or at ambient temperature. The comparison of gene expression between the two lines and heat treatments suggests that expression of 325 genes were significantly changed in modern broilers under heat stress, while only 3 genes changed their expression in …


Comment On Faa Rule Revision - Transport Category Aircraft, Paul F. Eschenfelder, Valter Battistoni Nov 2015

Comment On Faa Rule Revision - Transport Category Aircraft, Paul F. Eschenfelder, Valter Battistoni

Paul F. Eschenfelder

No abstract provided.


Phytogenic Feed Additives As An Alternative To Antibiotic Growth Promoters In Broiler Chickens, G. R. Murugesan, Basharat Syed, Sudipto Haldar, Chasity Pender Aug 2015

Phytogenic Feed Additives As An Alternative To Antibiotic Growth Promoters In Broiler Chickens, G. R. Murugesan, Basharat Syed, Sudipto Haldar, Chasity Pender

G. R. Murugesan

The recent trend toward reduction of antibiotic growth promoters (AGP) in North American poultry diets has put tremendous pressure on the industry to look for viable alternatives. In this context, phytogenic feed additives (PFA) are researched to improve gut health and thereby performance. An experiment was conducted with the objective to evaluate the effects of PFA as an alternative to AGP on small intestinal histomorphology, cecal microbiota composition, nutrient digestibility, and growth performance in broiler chickens. A total of 432, day-old Vencobb 400 broiler chicks were randomly assigned to one of three dietary groups, each consisting of 12 replicate pens …


In Ovo Serial Skeletal Muscle Diffusion Tractography Of The Developing Chick Embryo Using Dti: Feasibility And Correlation With Histology, Zien Zhou, Zachary Delproposto, Lianming Wu, Jianrong Xu, Jia Hua, Yan Zhou, Yongquan Ye, Zishu Zhang, Jiani Hu, E Mark Haacke Jun 2015

In Ovo Serial Skeletal Muscle Diffusion Tractography Of The Developing Chick Embryo Using Dti: Feasibility And Correlation With Histology, Zien Zhou, Zachary Delproposto, Lianming Wu, Jianrong Xu, Jia Hua, Yan Zhou, Yongquan Ye, Zishu Zhang, Jiani Hu, E Mark Haacke

Yan Zhou 周彦文档

Abstract Background Magnetic resonance imaging is a noninvasive method of evaluating embryonic development. Diffusion tensor imaging (DTI), based on the directional diffusivity of water molecules, is an established method of evaluating tissue structure. Yet embryonic motion degrades the in vivo acquisition of long-duration DTI. We used a dual-cooling technique to avoid motion artifact and aimed to investigate whether DTI can be used to monitor chick embryonic skeletal muscle development in ovo, and to investigate the correlation between quantitative DTI parameters fractional anisotropy (FA) and fiber length and quantitative histologic parameters fiber area percentage (FiberArea%) and limb length. Results From 84 …


A Critical Review Of Electrical Water-Bath Stun Systems For Poultry Slaughter And Recent Developments In Alternative Technologies, Sara J. Shields, A. B. M. Raj Jun 2015

A Critical Review Of Electrical Water-Bath Stun Systems For Poultry Slaughter And Recent Developments In Alternative Technologies, Sara J. Shields, A. B. M. Raj

Sara Shields, PhD

Prior to slaughter, most farmed birds move through a constant-voltage, multiple- bird, electrical water-bath stun system. Using this system subjects live birds to stressful and painful shackling, and the potential exists for them to receive prestun electric shocks and induction of seizures while still conscious. The existing elec- trical water-bath stunner settings, particularly those used in U.S. slaughter plants, are not necessarily based on sound scientific data that they produce a consistent, immediate stun, and research indicates that they are not effective in all birds. Further, in multiple-bird, electrical water-bath systems, birds may miss the stunner completely. Evidence suggests that …


A Comparison Of The Welfare Of Hens In Battery Cages And Alternative Systems, Sara Shields, Ian J.H. Duncan Jun 2015

A Comparison Of The Welfare Of Hens In Battery Cages And Alternative Systems, Sara Shields, Ian J.H. Duncan

Sara Shields, PhD

Housing systems for egg-laying hens range from small, pasture-based flocks to large, commercial-scale operations that intensively confine tens of thousands of hens indoors. The overwhelming majority of laying hens used for commercial egg production in the United States are confined in battery cages and provided 432.3 cm2 (67 in2) of space per bird. Cages prevent hens from performing the bulk of their natural behavior, including nesting, perching, dustbathing, scratching, foraging, exercising, running, jumping, flying, stretching, wingflapping, and freely walking. Cages also lead to severe disuse osteoporosis due to lack of exercise. Alternative, cage-free systems allow hens to move freely through …


Differential Expression Of Cell Cycle Regulators During Hyperplastic And Hypertrophic Growth Of Broiler Subcutaneous Adipose Tissue.Pdf, Jibin Zhang, Yeunsu Suh, Young Min Choi, Paula Chen, Michael Davis, Kichoon Lee May 2015

Differential Expression Of Cell Cycle Regulators During Hyperplastic And Hypertrophic Growth Of Broiler Subcutaneous Adipose Tissue.Pdf, Jibin Zhang, Yeunsu Suh, Young Min Choi, Paula Chen, Michael Davis, Kichoon Lee

Jibin Zhang

Hyperplasticgrowth and hypertrophicgrowth within adiposetissue is tightly associated with cellcycle activity. In this study, CCNG2 and CDKN2C were found to be correlated with cellcycle inhibition during fat cell differentiation, whereas CCND3, CCNA1, and ANAPC5 were positively associated with cellcycle activity during fat cell proliferation after selection based on GEO datasets available on the NCBI website. The findings were validated through comparison of expressions of these genes among different tissues/fractions in broiler chickens and time points during primary cell culture using quantitative real-time PCR. Development of broilersubcutaneousadiposetissue was investigated on embryonic days 15 and 17 and on post-hatch days 0, 5, …


The Human/Animal Interface: Emergence And Resurgence Of Zoonotic Infectious Diseases, Michael Greger May 2014

The Human/Animal Interface: Emergence And Resurgence Of Zoonotic Infectious Diseases, Michael Greger

Michael Greger, MD, FACLM

Emerging infectious diseases, most of which are considered zoonotic in origin, continue to exact a significant toll on society. The origins of major human infectious diseases are reviewed and the factors underlying disease emergence explored. Anthropogenic changes, largely in land use and agriculture, are implicated in the apparent increased frequency of emergence and reemergence of zoonoses in recent decades. Special emphasis is placed on the pathogen with likely the greatest zoonotic potential, influenzavirus A.


Modeling Parasitism Rate And Parasitism Risk: An Illustration Using A Colonially Nesting Songbird, The Red-Winged Blackbird Agelaius Phoeniceus, Max Post Van Der Burg, Larkin A. Powell, Andrew J. Tyre Apr 2013

Modeling Parasitism Rate And Parasitism Risk: An Illustration Using A Colonially Nesting Songbird, The Red-Winged Blackbird Agelaius Phoeniceus, Max Post Van Der Burg, Larkin A. Powell, Andrew J. Tyre

Andrew J Tyre

Ornithologists interested in the drivers of nest success and brood parasitism benefit from the development of new analytical approaches. One example is the development of so-called “log exposure” models for analyzing nest success. However, analyses of brood parasitism data have not kept pace with developments in nest success analyses. The standard approach uses logistic regression which does not account for multiple parasitism events, nor does it prevent bias from using observed proportions of parasitized nests. Likewise, logistic regression analyses do not capture fine scale temporal variation in parasitism. At first glance, it might be tempting to apply log exposure models …


A Study Of Mexican Free-Tailed Bat Chirp Syllables: Bayesian Functional Mixed Modeling Of Nonstationary Time Series Data With Time-Dependent Spectra, Josue G. Martinez, Kirsten M. Bohn, Raymond J. Carroll, Jeffrey S. Morris Feb 2013

A Study Of Mexican Free-Tailed Bat Chirp Syllables: Bayesian Functional Mixed Modeling Of Nonstationary Time Series Data With Time-Dependent Spectra, Josue G. Martinez, Kirsten M. Bohn, Raymond J. Carroll, Jeffrey S. Morris

Jeffrey S. Morris

We describe a new approach to analyze chirp syllables of free-tailed bats from two regions of Texas in which they are predominant: Austin and College Station. Our goal is to characterize any systematic regional differences in the mating chirps and assess whether individual bats have signature chirps. The data are analyzed by modeling spectrograms of the chirps as responses in a Bayesian functional mixed model. Given the variable chirp lengths, we compute the spectrograms on a relative time scale interpretable as the relative chirp position, using a variable window overlap based on chirp length. We use 2D wavelet transforms to …


New Peril + Old Promises = Bad Results, Paul Eschenfelder Feb 2013

New Peril + Old Promises = Bad Results, Paul Eschenfelder

Paul F. Eschenfelder

No abstract provided.


Erau Spring 2013 Newsletter, Paul Eschenfelder Jan 2013

Erau Spring 2013 Newsletter, Paul Eschenfelder

Paul F. Eschenfelder

No abstract provided.


Avma Guidelines For The Euthanasia Of Animals: 2013 Edition, Steven Leary, Wendy Underwood, Raymond Anthony, Samuel Cartner, Douglas Corey, Temple Grandin, Cheryl B. Greenacre, Sharon Gwaltney-Bran, Mary Ann Mccrackin, Robert Meyer, David Miller, Jan Shearer, Roy Yanong Jan 2013

Avma Guidelines For The Euthanasia Of Animals: 2013 Edition, Steven Leary, Wendy Underwood, Raymond Anthony, Samuel Cartner, Douglas Corey, Temple Grandin, Cheryl B. Greenacre, Sharon Gwaltney-Bran, Mary Ann Mccrackin, Robert Meyer, David Miller, Jan Shearer, Roy Yanong

Cheryl B Greenacre DVM

No abstract provided.


Influence Of Latitude On The Winter Abundance Of Red-Tailed Hawks (Buteo Jamaicensis) And American Kestrels (Falco Sparverius) In Illinois, Given Harper, Anna Groves, Vic Berardi, Paul Sweet, Jance Sweet, Angelo P. Capparella Dec 2012

Influence Of Latitude On The Winter Abundance Of Red-Tailed Hawks (Buteo Jamaicensis) And American Kestrels (Falco Sparverius) In Illinois, Given Harper, Anna Groves, Vic Berardi, Paul Sweet, Jance Sweet, Angelo P. Capparella

Given Harper

We use five years of data from 18 routes surveyed to determine the temporal stability of a first reported 100 years pattern, and reconfirmed that the abundance of winter 50 years ago tailed Hawk and American Kestrel decreases with increasing latitude, being more high in the central regions of Illinos in the northern regions. Trained volunteers conducted sampling ( n  = 143) a month driving along selected routes from December to February, from 2004-2005 to 2008-2009. We found significant increases in the abundance of both species from the northern regions to the central regions of Illinois. No significant effects of …


Rocky Mountain Birds: Birds And Birding In The Central And Northern Rockies, Paul A. Johnsgard Jul 2012

Rocky Mountain Birds: Birds And Birding In The Central And Northern Rockies, Paul A. Johnsgard

Paul Johnsgard

“The Rocky Mountain region has fascinated me ever since I traveled to Glacier and Yellowstone National Parks as a teenager, and saw for the first time such wonderful birds as ospreys, American dippers, and Lew­is’s woodpeckers.” This book is in part based on the author’s earlier Birds of the Rocky Moun­tains (1986, revised 2009), but over a third of the original text has been eliminated. The rest has been updated, expanded and modified to be less technical and more useful to birders in the field. Bird enthusiasts will find viewing loca­tions and updated contact information for hundreds of sites in …


The Art And Artistic Legacy Of Louis Agassiz Fuertes, Paul A. Johnsgard Jul 2012

The Art And Artistic Legacy Of Louis Agassiz Fuertes, Paul A. Johnsgard

Paul Johnsgard

Perhaps some Nebraska birders will not immediately recognize the name Louis Agassiz Fuertes, as he died almost 80 years ago. Yet he influenced the art of bird painting as much as did John James Audubon, and provided wonderful artwork for many major state bird reference books. He also personally tutored George Miksch Sutton, the Nebraska-born artist and biologist who provided the NOU with its Burrowing Owl logo, and who contributed greatly to American ornithology, especially that of the southern Great Plains. It is interesting that, like John James Audubon, Roger Tory Peterson, and George Miksch Sutton, we would never think …


A Nebraska Bird-Finding Guide, Paul A. Johnsgard Jul 2012

A Nebraska Bird-Finding Guide, Paul A. Johnsgard

Paul Johnsgard

Nebraska lies in the transition zone between North American eastern and western avifaunas and is home to more than 200 breeding and 150 migrant species. This definitive guide to Nebraska birdwatching by the state’s preeminent ornithologist includes a county-by-county rundown of the best sites, a calendar of migrations, an annotated checklist of regularly occurring Nebraska birds, and recommendations for optical equipment, publications and reference materials, and contact information for conservation and ornithological groups. It features 48 maps as well as photographs and drawings by the author. Paul Johnsgard, Foundation Professor Emeritus of Biological Sciences at the University of Nebraska–Lincoln, is …


Review Of 'My Way To Ornithology' By Olin Pettingill, Jr., Paul A, Johnsgard Jul 2012

Review Of 'My Way To Ornithology' By Olin Pettingill, Jr., Paul A, Johnsgard

Paul Johnsgard

Most Nebraskans who recognize this author's name probably will remember him as a cinematographer and lecturer in the Audubon Screen Tour series. The series was a regular part of live entertainment in Lincoln and Omaha until the early 1960s. Other bird-oriented people will know that Pettingill wrote two of the first and still best regional bird-finding books (detailed guidebooks to bird-finding localities in the states east and west of the Mississippi, respectively). Perhaps still others will remember him as a one-time teacher of ornithology at Carleton College and the University of Michigan Biological Station, or as a director of the …


Historic Birds Of Lincoln's Salt Basin Wetlands And Nine-Mile Prairie, Paul A. Johnsgard Jul 2012

Historic Birds Of Lincoln's Salt Basin Wetlands And Nine-Mile Prairie, Paul A. Johnsgard

Paul Johnsgard

The changes that have occurred in the bird life of the Lincoln area during the past century must certainly be great, but we have little evidence to document this point. There is, however, an annotated bird list from 1900 for the salt basin wetlands of western Lincoln, an area then gradually being developed for recreational use. This list was published by J. S. Hunter in the Proceedings of the First Annual Meeting of the Nebraska Ornithologists' Union (1900, 18-21). At this time, the recently impounded but still saline lake was 2 to 3 feet deep, and it covered about two …


Erau Aviation Wildlife Hazard Newsletter, Paul F. Eschenfelder Dec 2011

Erau Aviation Wildlife Hazard Newsletter, Paul F. Eschenfelder

Paul F. Eschenfelder

No abstract provided.


Aviation Wildlife Hazard - Uk Flight Safety Committee, Paul F. Eschenfelder Sep 2011

Aviation Wildlife Hazard - Uk Flight Safety Committee, Paul F. Eschenfelder

Paul F. Eschenfelder

No abstract provided.


High Speed Flight At Low Altitude: Hazard To Commercial Aviation ?, Paul F. Eschenfelder , Capt. Mar 2011

High Speed Flight At Low Altitude: Hazard To Commercial Aviation ?, Paul F. Eschenfelder , Capt.

Paul F. Eschenfelder

Commercial aircraft are capable of, and in fact, do, operate at high speed (>250 knots indicated airspeed [KIAS]) at low altitude (below 10,000’ above ground level) worldwide. Design, construction and certification standards for these aircraft were developed over 40 years ago. Since the development of these standards populations of large flocking birds have increased dramatically in many parts of the world. Yet neither design/construction standards nor operational practice have changed to reflect the new threat. Subsequent serious damage resulting from recent collisions indicates change is necessary. Since 2003, flight rules in Canada and the United States have been amended, …


Integrating Avian Radar Into The Aviation Operating Environment, Richard Sowden, Paul Eschenfelder Mar 2011

Integrating Avian Radar Into The Aviation Operating Environment, Richard Sowden, Paul Eschenfelder

Paul F. Eschenfelder

Avian radar technology has matured to the point where robust data and analysis tools are now able to provide the aviation industry with high quality information to support bird strike risk mitigation activities. The aviation operating environment is dynamic and challenging with complex interactions between the primary bird strike risk mitigation stakeholders; airport operators, air traffic service providers and flight crews. The transfer of this proof of concept technology into a suite of tools that is integrated into the aviation industry requires the engagement and support of the user community in the next critical evolutionary step of this emerging technology. …


Erau Aviation Wildlife Hazard Newsletter, Paul Eschenfelder Jan 2011

Erau Aviation Wildlife Hazard Newsletter, Paul Eschenfelder

Paul F. Eschenfelder

No abstract provided.


Development Of Endothermy In Nestling Bank Swallows (Riparia Riparia), Richard L. Marsh Dec 2010

Development Of Endothermy In Nestling Bank Swallows (Riparia Riparia), Richard L. Marsh

Richard Marsh

Body temperature (Tb) measurements after exposure to air temperatures (Tₐ) of 20 C or 27 C for 2 h in conjunction with metabolism measurements were used to describe the timing of and basis for developing temperature regulation in nestling swallows. As growth proceeds from hatching to 10 g there is a gradual increase in the Tb after exposure to low Tₐ. As growth continues beyond this point, the ability of nestlings to maintain Tb above Tₐ improves rapidly, such that nestlings weighing over 14 g are completely homeothermic when exposed to 20 C. Conductance (C, in cal [g⋅h⋅°C]⁻¹) decreases throughout …


Effects On Nestling Age And Burrow Depth On Co₂ And O₂ Concentrations In The Burrows Of Bank Swallows (Riparia Riparia), Steven J. Wickler, Richard L. Marsh Dec 2010

Effects On Nestling Age And Burrow Depth On Co₂ And O₂ Concentrations In The Burrows Of Bank Swallows (Riparia Riparia), Steven J. Wickler, Richard L. Marsh

Richard Marsh

Gas samples were taken from the nest chambers of bank swallows (Riparia riparia) and analyzed for CO₂ and O₂ content. The mean CO₂ content was 2.62% with a maximum value of 5.58%, and the mean O₂ content was 17.83% with a minimum value of 14.61%. There was a positive and significant correlation of increasing CO₂ content with both increasing nestling age and increasing total metabolizing mass (adults plus young). With increasing metabolizing mass there is a linear increase in CO₂ content, which suggests no active regulation of gas concentrations. Burrow depth also affected CO₂, particularly in burrows with older nestlings. …


Winter Fattening In The American Goldfinch And The Possible Role Of Temperature In Its Regulation, William R. Dawson, Richard L. Marsh Dec 2010

Winter Fattening In The American Goldfinch And The Possible Role Of Temperature In Its Regulation, William R. Dawson, Richard L. Marsh

Richard Marsh

We investigated whether environmental temperature has any causal role in the winter fattening in certain finches of the subfamily Carduelinae. Correlational analyses between fat content of American goldfinches (Carduelis tristis) and various short- and long-term measures of temperature provide no evidence for a proximate role of this environmental variable in determining the degree of fattening of these birds in southeastern Michigan. Their fat content shows the best correlations (r = −.61 to −.63) with the long-term average minimum temperature or record low temperature for the date of capture. Furthermore, inclusion of long-term thermal measures in multivariate analyses excludes from significance …