Life Sciences Commons^™

Probiotics And Antimicrobial Effect Of Lactiplantibacillus Plantarum, Saccharomyces Cerevisiae, And Bifidobacterium Longum Against Common Foodborne Pathogens In Poultry, Joy Igbafe, Agnes Kilonzo-Nthenge, Samuel N. Nahashon, Abdullah Ibn Mafiz, Maureen Nzomo

Agricultural and Environmental Sciences Faculty Research

The probiotic potential and antimicrobial activity of Lactiplantibacillus plantarum, Saccharomyces cerevisiae, and Bifidobacterium longum were investigated against Escherichia coli O157:H7, Salmonella typhimurium and Listeria monocytogenes. Selected strains were subjected to different acid levels (pH 2.5–6.0) and bile concentrations (1.0–3.0%). Strains were also evaluated for their antimicrobial activity by agar spot test. The potential probiotic strains tolerated pH 3.5 and above without statistically significant growth reduction. However, at pH 2.5, a significant (p < 0.05) growth reduction occurred after 1 h for L. plantarum (4.32 log CFU/mL) and B. longum (5.71 log CFU/mL). S. cerevisiae maintained steady cell counts for the entire treatment period without a statistically significant (p > 0.05) reduction (0.39 log CFU/mL). The results indicate at 3% bile concertation, 1.86 log CFU/mL reduction was observed for L. plantarum, while S. cerevisiae, and B. longum growth …

Transcriptome Analysis And Expression Of Selected Cationic Amino Acid Transporters In The Liver Of Broiler Chicken Fed Diets With Varying Concentrations Of Lysine, Collins N. Khwatenge, Boniface M. Kimathi, Samuel N. Nahashon

Agricultural and Environmental Sciences Faculty Research

Amino acids are known to play a key role in gene expression regulation. Amino acid signaling is mediated via two pathways: the mammalian target of rapamycin complex 1 (mTORC1) and the amino acid responsive (AAR) pathways. Cationic amino acid transporters (CATs) are crucial in these pathways due to their sensing, signaling and transport functions. The availability of certain amino acids plays a key role in the intake of other amino acids, hence affecting growth in young birds. However, the specific mechanism for regulating lysine transport for growth is not clear. In this study, we analyze the transcriptome profiles and mRNA …

Transcriptome Analyses Of Liver In Newly-Hatched Chicks During The Metabolic Perturbation Of Fasting And Re-Feeding Reveals Thrspa As The Key Lipogenic Transcription Factor, Larry A. Cogburn, Nares Trakooljul, Xiaofei Wang, Laura E. Ellestad, Tom E. Porter

Biology Faculty Research

Background

The fasting-refeeding perturbation has been used extensively to reveal specific genes and metabolic pathways that control energy metabolism in the chicken. Most global transcriptional scans of the fasting-refeeding response in liver have focused on juvenile chickens that were 1, 2 or 4 weeks old. The present study was aimed at the immediate post-hatch period, in which newly-hatched chicks were subjected to fasting for 4, 24 or 48 h, then refed for 4, 24 or 48 h, and compared with a fully-fed control group at each age (D1-D4).

Results

Visual analysis of hepatic gene expression profiles using hierarchical and K-means …