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Full-Text Articles in Life Sciences

Weed Presence Altered Biotic Stress And Light Signaling In Maize Even When Weeds Were Removed Early In The Critical Weed‐Free Period, David P. Horvath, Stephanie Bruggeman, Janet Moriles-Miller, James V. Anderson, Munevver Dogramaci, Brian E. Scheffler, Alvaro G. Hernandez, Michael E. Foley, Sharon Clay Apr 2018

Weed Presence Altered Biotic Stress And Light Signaling In Maize Even When Weeds Were Removed Early In The Critical Weed‐Free Period, David P. Horvath, Stephanie Bruggeman, Janet Moriles-Miller, James V. Anderson, Munevver Dogramaci, Brian E. Scheffler, Alvaro G. Hernandez, Michael E. Foley, Sharon Clay

Agronomy, Horticulture and Plant Science Faculty Publications

Weed presence early in the life cycle of maize (typically, from emergence through the 8 to 12 leaf growth stage) can reduce crop growth and yield and is known as the critical weed‐free period (CWFP). Even if weeds are removed during or just after the CWFP, crop growth and yield often are not recoverable. We compared transcriptome responses of field‐grown hybrid maize at V8 in two consecutive years among plants grown under weed‐free and two weed‐stressed conditions (weeds removed at V4 or present through V8) using RNAseq analysis techniques. Compared with weed‐free plant responses, physiological differences at V8 were identified …


An Epigenetic Breeding System In Soybean For Increased Yield And Stability, Sunil K. Kenchanmane Raju, Mon-Ray Shao, Robersy Sanchez, Ying-Zhi Xu, Ajay Sandhu, George L. Graef, Sally A. Mackenzie Feb 2018

An Epigenetic Breeding System In Soybean For Increased Yield And Stability, Sunil K. Kenchanmane Raju, Mon-Ray Shao, Robersy Sanchez, Ying-Zhi Xu, Ajay Sandhu, George L. Graef, Sally A. Mackenzie

Department of Agronomy and Horticulture: Faculty Publications

Epigenetic variation has been associated with a wide range of adaptive phenotypes in plants, but there exist few direct means for exploiting this variation. RNAi suppression of the plant-specific gene, MutS HOMOLOG1 (MSH1), in multiple plant species produces a range of developmental changes accompanied by modulation of defence, phytohormone and abiotic stress response pathways along with methylome repatterning. This msh1-conditioned developmental reprogramming is retained independent of transgene segregation, giving rise to transgene-null ‘memory’ effects. An isogenic memory line crossed to wild type produces progeny families displaying increased variation in adaptive traits that respond to selection. This study …


Phytobiome And Transcriptional Adaptation Of Populus Deltoides To Acute Progressive Drought And Cyclic Drought, Benjamin Garcia, Jessy Labbé, Piet Jones, Piet Jones, Paul Abraham, Ian Hodge, Ian Hodge, Sharlee Climer, Sara Jawdy, Lee Gunter, Gerald Tuskan, Xiaohan Yang, Timothy Tschaplinski, Daniel Jacobson, Daniel Jacobson Jan 2018

Phytobiome And Transcriptional Adaptation Of Populus Deltoides To Acute Progressive Drought And Cyclic Drought, Benjamin Garcia, Jessy Labbé, Piet Jones, Piet Jones, Paul Abraham, Ian Hodge, Ian Hodge, Sharlee Climer, Sara Jawdy, Lee Gunter, Gerald Tuskan, Xiaohan Yang, Timothy Tschaplinski, Daniel Jacobson, Daniel Jacobson

Computer Science Faculty Works

Plant drought stress causes systematic changes to photosynthesis, metabolism, growth, and potentially the phytobiome. Additionally, drought affects plants in both a species-specific and water-deficit-driven manner, causing the response to drought to be dependent both on how drought is being experienced and on any adaptation to prior drought exposure. Thus, understanding the effect of drought on plants requires assessing drought response in multiple conditions, such as progressive acute drought and recurrent cyclic drought, and at different levels of severity. In this study, we have utilized RNA sequencing to identify changes to the plant transcriptome and the phytobiome during both acute progressive …


Transcriptional Reprogramming Of Legume Genomes: Perspective And Challenges Associated With Single-Cell And Single Cell-Type Approaches During Nodule Development, Marc Libault Jan 2018

Transcriptional Reprogramming Of Legume Genomes: Perspective And Challenges Associated With Single-Cell And Single Cell-Type Approaches During Nodule Development, Marc Libault

Department of Agronomy and Horticulture: Faculty Publications

Transcriptomic approaches revealed thousands of genes differentially or specifically expressed during nodulation, a biological process resulting from the symbiosis between leguminous plant roots and rhizobia, atmospheric nitrogen-fixing symbiotic bacteria. Ultimately, nodulation will lead to the development of a new root organ, the nodule. Through functional genomic studies, plant transcriptomes have been used by scientists to reveal plant genes potentially controlling nodulation. However, it is important to acknowledge that the physiology, transcriptomic programs, and biochemical properties of the plant cells involved in nodulation are continuously regulated. They also differ between the different cell-types composing the nodules. To generate a more accurate …


Transcriptome Analysis Of Root Development In Wheat Triticum Aestivum Using High Throughtput Sequencing Technologies, Ghana Challa Jan 2018

Transcriptome Analysis Of Root Development In Wheat Triticum Aestivum Using High Throughtput Sequencing Technologies, Ghana Challa

Electronic Theses and Dissertations

Root provides plant water, nutrients and anchorage from soil. Most our knowledge of molecular mechanisms of root development is from the dicot model plant Arabidopsis, but very few studies have done in monocot crop systems like rice, maize, and wheat. We are studying very short root (VSR) phenotype in wheat, and lack of a sequenced reference genome in wheat prompted us to sequence and assemble the root transcriptome of the reference cultivar Chinese Spring (CS). A root transcriptome was assembled from the sequenced reads generated from root tip and the mature root tissues of CS. Approximately 169 million reads were …


Microbial Communities And Their Impact On Bioenergy Crops In Dynamic Environments, Brandon Monier Jan 2018

Microbial Communities And Their Impact On Bioenergy Crops In Dynamic Environments, Brandon Monier

Electronic Theses and Dissertations

Native perennial grasses, such as prairie cordgrass (PCG, Spartina pectinata Link), and switchgrass (SG, Panicum virgatum L.) have a great potential as bioenergy crops, because they require fewer inputs, produce more energy, and reduce greenhouse gas emissions in comparison to annual cropping systems such as corn and soybean. SG has been selected by the U.S. Department of Energy for development as bioenergy crop, but studies have shown that PCG can outcompete switchgrass in terms of biomass production. These crops can also form associations with a wide variety of plant growth promoting microbes including arbuscular mycorrhial (AM) fungi. In CHAPTER 2, …