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Full-Text Articles in Life Sciences

Msh1-Mediated Non-Genetic Phenotypic Variation In Plants: Prospects For Epigenetic Breeding In Sorghum Bicolor, Dikungwa Ketumile Aug 2017

Msh1-Mediated Non-Genetic Phenotypic Variation In Plants: Prospects For Epigenetic Breeding In Sorghum Bicolor, Dikungwa Ketumile

Department of Agronomy and Horticulture: Dissertations, Theses, and Student Research

Sorghum is the fifth most important cereal crop grown in the world, and is the primary staple food for many people in developing countries. Like other major crops, its improvement is constrained by depletion of genetic diversity within breeding germplasm. Investigated in this study is the unique creation of non-genetic phenotypic variation through altering MutS Homolog1 (MSH1), a plant-specific gene, and the potential it presents for sorghum improvement. Suppression of MSH1 results in non-genetic developmental reprogramming. The derived MSH1 memory lines, when used in crossing, result in heritable phenotypic variation that enhances plant vigor and agronomic performance. A …


Interaction Of Seeding And Nitrogen Rate On Grain Sorghum Yield In Southwest Kansas, A. J. Foster, A. Schlegel, J. D. Holman, I. A. Ciampitti, C. Thompson, D. Ruiz Diaz Jun 2017

Interaction Of Seeding And Nitrogen Rate On Grain Sorghum Yield In Southwest Kansas, A. J. Foster, A. Schlegel, J. D. Holman, I. A. Ciampitti, C. Thompson, D. Ruiz Diaz

Kansas Agricultural Experiment Station Research Reports

This study compared drilled planted sorghum at four seeding rates to planted sorghum at three different nitrogen (N) fertility levels at two locations in southwest Kansas (Garden City and Tribune). At the Garden City location, no difference was observed in yield among the drilled seeded sorghum populations greater than 27,000 seeds/a compared to the standard planted sorghum (sorghum planted at 27,000 seeds/a with a planter at 30 in.-row spacing). At Tribune, there was no difference in yield between the drilled sorghum and the standard planted sorghum (sorghum planted at 40,000 seeds/a with a planter at 30 in.-row spacing) regardless of …


Palmer Amaranth (Amaranthus Palmeri) Suppression With Half Rates Of Dicamba And Atrazine With Increasing Sorghum (Sorghum Bicolor) Density And Nitrogen Rate, I. B. Cuvaca, R. Currie, A. J. Foster Jun 2017

Palmer Amaranth (Amaranthus Palmeri) Suppression With Half Rates Of Dicamba And Atrazine With Increasing Sorghum (Sorghum Bicolor) Density And Nitrogen Rate, I. B. Cuvaca, R. Currie, A. J. Foster

Kansas Agricultural Experiment Station Research Reports

Palmer amaranth (PA) competition can result in severe yield loss in grain sorghum. Increasing sorghum density and nutrient supply could promote early/rapid canopy closure and therefore reduce the amount of light that could otherwise penetrate the canopy and promote PA growth in sorghum. A study was conducted at the Southwest Research-Extension Center near Garden City, KS, to determine if PA could be suppressed with dicamba and atrazine applied as PRE at half rates combined with increasing sorghum density (60,000, 90,000, and 120,000 seeds/a), and nitrogen rate (0, 100, 200 lb/a). Preliminary results indicate that increasing plant density and nitrogen rate …


A Comprehensive Analysis Of Alternative Splicing In Paleopolyploid Maize, Wenbin Mei, Sanzhen Liu, James C. Schnable, Cheng-Ting Yeh, Nathan M. Springer, Patrick S. Schnable, William Brad Barbazuk May 2017

A Comprehensive Analysis Of Alternative Splicing In Paleopolyploid Maize, Wenbin Mei, Sanzhen Liu, James C. Schnable, Cheng-Ting Yeh, Nathan M. Springer, Patrick S. Schnable, William Brad Barbazuk

Department of Agronomy and Horticulture: Faculty Publications

Identifying and characterizing alternative splicing (AS) enables our understanding of the biological role of transcript isoform diversity. This study describes the use of publicly available RNA-Seq data to identify and characterize the global diversity of AS isoforms in maize using the inbred lines B73 and Mo17, and a related species, sorghum. Identification and characterization of AS within maize tissues revealed that genes expressed in seed exhibit the largest differential AS relative to other tissues examined. Additionally, differences in AS between the two genotypes B73 and Mo17 are greatest within genes expressed in seed. We demonstrate that changes in the level …


Field-Observed Angles Of Repose For Stored Grain In The United States, Rumela Bhadra, Mark E. Casada, Sidney A. Thompson, Josephine M. Boac, Ronaldo G. Maghirang, Michael D. Montross, Aaron P. Turner, Samuel G. Mcneill Jan 2017

Field-Observed Angles Of Repose For Stored Grain In The United States, Rumela Bhadra, Mark E. Casada, Sidney A. Thompson, Josephine M. Boac, Ronaldo G. Maghirang, Michael D. Montross, Aaron P. Turner, Samuel G. Mcneill

Biosystems and Agricultural Engineering Faculty Publications

Bulk grain angle of repose (AoR) is a key parameter for inventorying grain, predicting flow characteristics, and designing bins and grain handling systems. The AoR is defined for two cases, piling (dynamic) or emptying (static), and usually varies with grain type. The objective of this study was to measure piling angles of repose for corn, sorghum, barley, soybeans, oats, and hard red winter (HRW) wheat in steel and concrete bins in the United States. Angles were measured in 182 bins and 7 outdoor piles. The piling AoR for corn ranged from 15.7° to 30.2° (median of 20.4° and standard deviation …


Validation Of Qtl Mapping And Transcriptome Profiling For Identification Of Candidate Genes Associated With Nitrogen Stress Tolerance In Sorghum, Malleswari Gelli, Anji Reddy Konda, Kan Liu, Chi Zhang, Thomas E. Clemente, David R. Holding, Ismail M. Dweikat Jan 2017

Validation Of Qtl Mapping And Transcriptome Profiling For Identification Of Candidate Genes Associated With Nitrogen Stress Tolerance In Sorghum, Malleswari Gelli, Anji Reddy Konda, Kan Liu, Chi Zhang, Thomas E. Clemente, David R. Holding, Ismail M. Dweikat

Department of Agronomy and Horticulture: Faculty Publications

Background: Quantitative trait loci (QTLs) detected in one mapping population may not be detected in other mapping populations at all the time. Therefore, before being used for marker assisted breeding, QTLs need to be validated in different environments and/or genetic backgrounds to rule out statistical anomalies. In this regard, we mapped the QTLs controlling various agronomic traits in a recombinant inbred line (RIL) population in response to Nitrogen (N) stress and validated these with the reported QTLs in our earlier study to find the stable and consistent QTLs across populations. Also, with Illumina RNA-sequencing we checked the differential expression of …


Expression Of The Maize Dof1 Transcription Factor In Wheat And Sorghum, Pamela A. Pena, Truyen Quach, Shirley Sato, Zhengxiang Ge, Natalya Nersesian, Taity Changa, Ismail M. Dweikat, Madhavan Soundararajan, Tom E. Clemente Jan 2017

Expression Of The Maize Dof1 Transcription Factor In Wheat And Sorghum, Pamela A. Pena, Truyen Quach, Shirley Sato, Zhengxiang Ge, Natalya Nersesian, Taity Changa, Ismail M. Dweikat, Madhavan Soundararajan, Tom E. Clemente

Department of Agronomy and Horticulture: Faculty Publications

Nitrogen is essential for plant growth and development. Improving the ability of plants to acquire and assimilate nitrogen more efficiently is a key agronomic parameter that will augment sustainability in agriculture. A transcription factor approach was pursued to address improvement of nitrogen use efficiency in two major commodity crops. To this end, the Zea mays Dof1 (ZmDof1) transcription factor was expressed in both wheat (Triticum aestivum) and sorghum (Sorghum bicolor) either constitutively, UBI4 promoter from sugarcane, or in a tissue specific fashion via the maize rbcS1 promoter. The primary transcription activation target of ZmDof1 …


Interaction Of Seeding And Nitrogen Rate On Grain Sorghum Yield In Southwest Kansas, A. J. Foster, A. Schlegel, J. D. Holman, I. A. Ciampitti, C. Thompson, Dorivar Ruiz Diaz Jan 2017

Interaction Of Seeding And Nitrogen Rate On Grain Sorghum Yield In Southwest Kansas, A. J. Foster, A. Schlegel, J. D. Holman, I. A. Ciampitti, C. Thompson, Dorivar Ruiz Diaz

Kansas Agricultural Experiment Station Research Reports

This study compared drilled planted sorghum at four seeding rates to planted sorghum at three different nitrogen (N) fertility levels at two locations in southwest Kansas (Garden City and Tribune). At the Garden City location, no difference was observed in yield among the drilled seeded sorghum populations greater than 27,000 seeds/a compared to the standard planted sorghum (sorghum planted at 27,000 seeds/a with a planter at 30 in.-row spacing). At Tribune, there was no difference in yield between the drilled sorghum and the standard planted sorghum (sorghum planted at 40,000 seeds/a with a planter at 30 in.-row spacing) regardless of …


Palmer Amaranth (Amaranthus Palmeri) Suppression With Half Rates Of Dicamba And Atrazine With Increasing Sorghum (Sorghum Bicolor) Density And Nitrogen Rate, I. B. Cuvaca, R. Currie, A. J. Foster Jan 2017

Palmer Amaranth (Amaranthus Palmeri) Suppression With Half Rates Of Dicamba And Atrazine With Increasing Sorghum (Sorghum Bicolor) Density And Nitrogen Rate, I. B. Cuvaca, R. Currie, A. J. Foster

Kansas Agricultural Experiment Station Research Reports

Palmer amaranth (PA) competition can result in severe yield loss in grain sorghum. Increasing sorghum density and nutrient supply could promote early/rapid canopy closure and therefore reduce the amount of light that could otherwise penetrate the canopy and promote PA growth in sorghum. A study was conducted at the Southwest Research-Extension Center near Garden City, KS, to determine if PA could be suppressed with dicamba and atrazine applied as PRE at half rates combined with increasing sorghum density (60,000, 90,000, and 120,000 seeds/a), and nitrogen rate (0, 100, 200 lb/a). Preliminary results indicate that increasing plant density and nitrogen rate …