Life Sciences Commons^™

Overexpression Of Sbmyb60 In Sorghum Bicolor Impacts Both Primary And Secondary Metabolism, Erin D. Scully, Tammy Gries, Nathan A. Palmer, Gautam Sarath, Deanna L. Funnell-Harris, Lisa Baird, Paul Twigg, Javier Seravalli, Thomas E. Clemente, Scott E. Sattler

Center for Plant Science Innovation: Faculty and Staff Publications

• Few transcription factors have been identified in C₄ grasses that either positively or negatively regulate monolignol biosynthesis.
• Previously, the overexpression of SbMyb60 in sorghum (Sorghum bicolor) has been shown to induce monolignol biosynthesis, which leads to elevated lignin deposition and altered cell wall composition. To determine how SbMyb60 overexpression impacts other metabolic pathways, RNA-Seq and metabolite profiling were performed on stalks and leaves.
• 35S::SbMyb60 was associated with the transcriptional activation of genes involved in aromatic amino acid, S-adenosyl methionine (SAM) and folate biosynthetic pathways. The high coexpression values between SbMyb60 and genes assigned to these pathways indicate …