Life Sciences Commons^™

Provitamin A Biofortification Of Cassava Enhances Shelf Life But Reduces Dry Matter Content Of Storage Roots Due To Altered Carbon Partitioning Into Starch, Getu Beyene, Felix R. Solomon, Raj D. Chauhan, Eliana Gaitán-Solis, Narayanan Narayanan, Jackson Gehan, Dimuth Siritunga, Robin L. Stevens, John Jifon, Joyce Van Eck, Edward Linsler, Malia Gehan, Muhammad Ilyas, Martin Fregene, Richard T. Sayre, Paul Anderson, Nigel Taylor, Edgar B. Cahoon

Center for Plant Science Innovation: Faculty and Staff Publications

Storage roots of cassava (Manihot esculenta Crantz), a major subsistence crop of sub-Saharan Africa, are calorie rich but deficient in essential micronutrients, including provitamin A β-carotene. In this study, β-carotene concentrations in cassava storage roots were enhanced by coexpression of transgenes for deoxy-d-xylulose-5-phosphate synthase (DXS) and bacterial phytoene synthase (crtB), mediated by the patatin-type 1 promoter. Storage roots harvested from field-grown plants accumulated carotenoids to ≤50 lg/g DW, 15- to 20-fold increases relative to roots from nontransgenic plants. Approximately 85%–90% of these carotenoids accumulated as all-trans-β-carotene, the most nutritionally efficacious carotenoid. β-Carotene-accumulating storage roots displayed delayed onset of postharvest …

Advanced Biotechnology Tools For Invasive Species Management, Invasive Species Advisory Committee

National Invasive Species Council

Increasingly, genetic tools are being used to detect and solve pressing environmental, social, and health-related challenges. It is clear that investments in technology innovation can be game changing, as advances in biotechnology may provide new methods to protect the nation’s resources from the negative impacts of invasive species. The current toolbox of management options is recognizably insufficient to deal with many of the high-impact species that have been introduced. However, “surrendering” to these species is generally not a viable option from ecological, health, economic, socio-cultural, or political perspectives. Cost-efficient solutions to these “grand invasive species challenges” need to be found. …

Managed Relocation: Reducing The Risk Of Biological Invasion, Edward E. Clark Jr., Dan Simberloff, Mark Schwartz, Brent Stewart, John Peter Thompson

National Invasive Species Council

Key Finding

Any organism that is relocated to a novel ecosystem has the potential to become an invasive species or spread “hitching” invasive species, or both. Managed Relocation is not congruent with Executive Order 13112 to the extent that it might facilitate “economic or environmental harm or harm to human, animal, or plant health.” Consequently, the actions by federal agencies or those entities supported by federal funding to engage in managed relocation need to be addressed in a manner consistent with EO 13751 Section 3(3), which compels Agencies to:

Refrain from authorizing, funding, or implementing actions that are likely to …

Enhancing Federal-Tribal Coordination Of Invasive Species, Blaine Parker, Chuck Bargeron, Sean Southey, Lori Buchanan, Miles Falck, Chris Fisher, Joe Maroney, Mervin Wright, Gintas Zavadkas

National Invasive Species Council

Invasive species are defined by the United States government to mean “with regard to a particular ecosystem, a non-native organism whose introduction causes or is likely to cause economic or environmental harm, or harm to human, animal, or plant health” (Executive Order [EO] 13751). The ecosystems to which invasive species are introduced or spread are not delimited by jurisdictional boundaries; they intersect with lands managed by federal, tribal, state, territorial, and county governments, as well as properties under private ownership. For this reason, effective coordination and cooperation across jurisdictions is of paramount importance in the prevention, eradication, and control of …

Avian Diversity, Abundance, And Nest Success Among Managed Prairies And Agricultural Plots In Oklahoma And Texas, Phillip J. Leonard, Douglas R. Wood, Wayne E. Meyer

The Prairie Naturalist

Over the last 50 years, grassland birds experienced rapid declines due to habitat loss and degradation as a result of agricultural practices. Our objective was to document the diversity, abundance, and nest success of bird communities using managed prairie and agricultural plots at the Tishomingo National Wildlife Refuge (NWR) in southern Oklahoma and Hagerman NWR in northern Texas. From April 1 to July 15, 2013–2014, point count surveys, nest searches, nest monitoring, and vegetation sampling were conducted among three habitat treatments: managed prairie, unharvested wheat, and fallow agricultural plots. Species richness values for potential nesting species were higher in managed …

Unique Nesting Behavior By Trumpeter Swans (Cygnus Buccinator), Heather M. Johnson, Michael Forsberg, Letitia Reichart, Mark P. Vrtiska

The Prairie Naturalist

Nesting behavior by Trumpeter swans (Cygnus buccinator) has been well documented for most populations. Both sexes cooperate in nest construction, which takes 11–35 days to complete (Hansen et al. 1971, Cooper 1979). Nests generally range from 1.5–3.6 meters in diameter and 1.5 meters in height and are typically built on solid structures such as a muskrat (Ondatra zibethicus) house or an island (Hansen et al. 1971, Cooper 1979). However, we discovered an atypical Trumpeter swan nest built of cattail (Typha angustifolia L.) located on Vaughn Lake, in Cherry County, Nebraska on 13 May 2016.

We monitored the atypical nest by …

One More Warbler: A Life With Birds., Laura Erickson

The Prairie Naturalist

Imagine going on a Victor Emanuel Nature Tour, seated in the van next to Victor Emanuel himself. Between birding stops, this man who has seen over 6,000 species as a world-renowned conservationist and innovator in ecotourism regales you with stories. He starts with his “spark bird”: when he was eight years old, he was taken with the beauty of a pair of Northern Cardinals (Cardinalis cardinalis), and was hooked. Whenever people pile back into the van after a birding stop, he starts another story.

That’s how One More Warbler: A Life with Birds reads, as if Emanuel sat down with …

Field Discrimination Of Prairie Deer Mice And White-Footed Mice Using Morphological Characteristics, Jacob L. Berl, Kelton M. Verble, Elizabeth A. Flaherty, Robert K.S. Swihart

The Prairie Naturalist

Field discrimination of prairie deer mice (Peromyscus maniculatus bairdii) and white-footed mice (P. leucopus noveboracensis) can be difficult throughout much of the central United States where they co-occur. We live-trapped prairie deer mice and white-footed mice within forested and row-crop habitats in central Indiana and used multiplex PCR with species-specific primers to positively determine species identification. We collected a suite of commonly measured external morphological traits (body weight and lengths of ear, hindfoot, tail, and body) from each captured animal. Individuals were assigned to species based on analysis of DNA; discriminant function analysis was used to …

The Great Plains Natural Science Society

The Prairie Naturalist

The Great Plains Natural Science Society, formerly the North Dakota Natural Science Society, was founded in 1967 and seeks to promote interest in and understanding of natural history in the Great Plains, to encourage the conservation of natural resources, and to provide communication among individuals, institutions, and organizations of like interests. The GPNSS actively promotes the study of natural history of the Great Plains region, including geology, plants, birds, mammals, fish, insects, and other forms of life. Together with local, state, and national conservation organizations, the GPNSS fosters natural resource conservation and preservation of outstanding natural areas. The GPNSS publishes …

Book Reviews: Raptors Of Mexico And Central America. William S. Clark And N. John Schmitt., Jack Jack Eitniear

The Prairie Naturalist

Whether a raptor is cryptically perched in the shadows or is viewed only as a silhouette soaring in the clouds below the glaring midday sun, raptor identification can be challenging. If you are observing raptors in the North Temperate Zone, several guides (e.g., Clark and Wheeler 2001 Dunne et al.2012) will aid you with such identifications, but few resources exist for visitors south of this zone. So, if you are interested in identifying the 69 species of diurnal raptors found in Mexico and Central America, the recently released Raptors of Mexico and Central America by William S. Clark and N. …

The Prairie Naturalist: The Journal Of The Great Plains Natural Science Society

The Prairie Naturalist

TABLE OF CONTENTS

45 EDITOR’S NOTE

RESEARCH ARTICLES

48 Avian Diversity, Abundance, and Nest Success among Managed Prairies and Agricultural Plots in Oklahoma and Texas Phillip J. Leonard, Douglas R. Wood, and Wayne E. Meyer

57 Severe Drought Impacts Female Pheasant Physiology in Southwest Nebraska Jessica A. Laskowski, Gwen C. Bachman, and Joseph J. Fontaine

66 Field Discrimination of Prairie Deer Mice and White-footed Mice using Morphological Characteristics Jacob L. Berl, Kelton M. Verble, Elizabeth A. Flaherty, and Robert K. Swihart

NOTES

72 Range Extension of the Virgina Opossum (Didelphis virginiana) in North Dakota Lisa L. Walsh

76 Prairie Falcon …

Energy Sprawl Solutions, Nicola Koper

The Prairie Naturalist

Energy sprawl affects all the major conservation issues of our age, from habitat loss and fragmentation to anthropogenic noise and climate change. As such, minimizing and solving its associated problems should be a key focus for conservation biologists. Kiesecker and Naugle provide an impressive and compact book that focusses on solving environmental and social problems likely to result from a transition from non- renewable to far less efficient renewable energy sources.

Energy Sprawl Solutions summarizes numerous relevant peer-reviewed academic papers, and outlines management and planning implications of each one, ultimately resolving these into fairly clear prescriptive recommendations for moving forward. …

Severe Drought Impacts Female Pheasant Physiology In Southwest Nebraska, Jessica A. Laskowski, Gwen C. Bachman, Joseph J. Fontaine

The Prairie Naturalist

In 2012, Nebraska experienced one of the worst droughts since the 1930s, accompanied by abnormally high temperatures. We studied the impacts of the 2012 summer drought on female ring-necked pheasant (Phasianus colchicus) body condition and baseline and stress-induced corticosterone concentrations (CORT). We hypothesized that drought conditions would reduce pheasant body condition, increase chronic stress resulting in elevated baseline CORT levels, and down-regulate pheasant stress response to acute stressors, resulting in reduced stress-induced CORT concentrations. In southwestern Nebraska, we captured female pheasants in 2012 (pre-drought) and 2013 (post-drought). Pheasants had poorer body condition after the drought. Although female CORT measures were …

A Swift Guide To Butterflies Of North America, Second Edition., Ann B. Swengel

The Prairie Naturalist

As soon as I got my hands on A Swift Guide to Butterflies of North America, I immediately checked the species account for Aphrodite Fritillary (Speyeria aphrodite). The prairie version of this species can be sufficiently different from the typically illustrated nominate subspecies such that it must be specifically explained as being distinct. This book passed my first test—describing details that were not only essential for identifying this fritillary species but also for realizing how similar its underside can be to our prairie specialty, the stunning Regal Fritillary (S. idalia).

Much of my research on butterflies occurs in grasslands. For …

Notes: Range Extension Of The Virginia Opossum (Didelphis Virginiana) In North Dakota, Lisa L. Walsh, Robert Seabloom, Cody W. Thompson

The Prairie Naturalist

The Virginia opossum (Didelphis virginiana) is broadly distributed across North America from Costa Rica in the south to southern Ontario in the north and from the southern Great Plains in the west to the eastern United States. The Virginia opossum also was introduced multiple times to thePacific Coast beginning in the late 1800s and has established populations in that region (Gardner and Sunquist 2003). This species is a habitat generalist known to frequent wetland and hardwood habitats but also can be found in grasslands, along forest edges, and in agricultural and suburban settings throughout its range (Gardner and Sunquist 2003, …

The Considerations Given To Determining Authorship, Christopher N. Jacques

The Prairie Naturalist

Greetings GPNSS members! Over the past year, I have been asked by prospective authors and colleagues whether The Prairie Naturalist has criteria for authorship. Given that this issue continues to arise, it is comforting to know that I am not the only one who struggles with considerations given to determining authorship. I checked the current submission guidelines and found nothing specific, which in turn motivated me to explore what other journals such as the Journal of Wildlife Management, Proceedings of the National Academy of Sciences, and Ecology recommend to prospective authors (Merrill 2015). Much to my surprise, discussions of authorship …

Prairie Falcon Depredation Attempts On A Greater Prairie-Chicken Lek In South-Central Nebraska, Andrew J. Caven, Joshua D. Wiese, William R. Wallauer

The Prairie Naturalist

Little information exists concerning Prairie falcons’ (Falco mexicanus; PRFA) seasonal movements, habitat use, and diet outside of the breeding season; this is especially true in the eastern portion of its wintering and migratory range (Steenhof 1998, Sharpe et al. 2001, Steenhof et al. 2005). Prairie falcons prey on ground squirrels (Spermophilus spp.), prairie dogs (Cynomys spp.), wood rats (Neotoma spp.), and other rodents during the breeding season (Bond 1936, 1942, MacLaren et al. 1988, Steenhof 1998, Steenhof et al. 1999, Johnsgard 2013). They utilize avian prey regionally as well, including western meadowlarks (Sturnella neglecta …

Horticulture For Pollinator Conservation, Carter M. Westerhold

Department of Agronomy and Horticulture: Dissertations, Theses, and Student Research

Pollinators worldwide are declining. Consequently, the agricultural and ecological services these insects provide are in danger of being lost. Land use intensification, via urbanization, has greatly influenced this decline in pollinators. Luckily, through targeted horticultural practices, stable populations of pollinators can be sustained within urban areas. The horticultural practices of planting diverse floral resources and managing pollinator habitat in urban areas can sustain these populations. Two studies were conducted with the intent to identify horticultural knowledge gaps that could be reduced to aid in pollinator conservation efforts. First, a study to compare Nebraska native and non-native ornamental plants was conducted. …

Influence Of An Internal Parasite Control On Cattle Grazing Behavior And Production, Jace Stott

Department of Agronomy and Horticulture: Dissertations, Theses, and Student Research

Six herds of 45 to 90 cow/calf pairs grazing on upland range were used to examine the efficacy of an injectable extended release eprinomectin parasite control on production traits, activity behavior, and efficacy against internal parasitism. In 2016, treatment cows were given a subcutaneous injection of LongRange^tm. In 2017, all cows in the study were treated with a short acting Synanthic^tm treatment and only treatment cows were given an additional LongRange^tm treatment. In both 2016 and 2017, fecal egg counts were significantly lower (P < 0.05) in eprinomectin treated cows compared to control cows. Calf gains were 4.8 kg and 8.7 kg greater (P < 0.1) for the calves of dams treated with eprinomectin compared to calves of control cows in 2016 and 2017, respectively. Activity characteristics of cattle were inconclusive, with treated cows having lower (P < 0.01) grazing and traveling times compared to control cows in 2016, and more (P < 0.01) grazing and traveling in 2017.

Differences in grazing behaviors based on time within pasture at different times …

Substantial Contribution Of Genetic Variation In The Expression Of Transcription Factors To Phenotypic Variation Revealed By Erd-Gwas, Hung-Ying Lin, Qiang Liu, Xiao Li, Jinliang Yang, Sanzhen Liu, Yinlian Huang, Michael J. Scanlon, Dan Nettleton, Patrick S. Schnable

Department of Agronomy and Horticulture: Faculty Publications

Background: There are significant limitations in existing methods for the genome-wide identification of genes whose expression patterns affect traits.

Results: The transcriptomes of five tissues from 27 genetically diverse maize inbred lines were deeply sequenced to identify genes exhibiting high and low levels of expression variation across tissues or genotypes. Transcription factors are enriched among genes with the most variation in expression across tissues, as well as among genes with higher-than-median levels of variation in expression across genotypes. In contrast, transcription factors are depleted among genes whose expression is either highly stable or highly variable across genotypes. We developed a …

Estimation Of Irrigation Requirements For Drip-Irrigated Maize In A Sub-Humid Climate, Liu Yang, Hai-Shun Yang, Jiu-Sheng Li, Yan-Feng Li, Hai-Jun Yan

Department of Agronomy and Horticulture: Faculty Publications

Drip-irrigation is increasingly applied in maize (Zea mays L.) production in sub-humid region. It is critical to quantify irrigation requirements during different growth stages under diverse climatic conditions. In this study, the Hybrid-Maize model was calibrated and applied in a sub-humid Heilongjiang Province in Northeast China to estimate irrigation requirements for drip irrigated maize during different crop physiological development stages and under diverse agro-climatic conditions. Using dimensionless scales, the whole growing season of maize was divided into diverse development stages from planting to maturity. Drip-irrigation dates and irrigation amounts in each irrigation event were simulated and summarized in 30-year simulation …

Identifying Ecologically Relevant Scales Of Habitat Selection: Diel Habitat Selection In Elk, Caleb P. Roberts, James W. Cain Iii, Robert D. Cox

Department of Agronomy and Horticulture: Faculty Publications

Although organisms make resource selection decisions at multiple spatiotemporal scales, not all scales are ecologically relevant to any given organism. Ecological patterns and rhythms such as behavioral and climatic patterns may provide a consistent method for identifying ecologically relevant scales of habitat selection. Using elk (Cervus canadensis) as an example species, we sought to test the ability of behavioral patterns to empirically partition diel scales for modeling habitat selection. We used model selection to partition diel scales by shifts in dominant behavior and then used resource selection probability functions to model elk habitat selection hierarchically at diel scales within seasons. …

Effects Of Nitrogen And Phosphorus Fertilizer And Topsoil Amendment On Native Plant Cover In Roadside Revegetation Projects, Heidi L. Hillhouse, Walter H. Schacht, Jonathan M. Soper, Carol E. Wienhold

Department of Agronomy and Horticulture: Faculty Publications

Establishing vegetation on roadsides following construction can be challenging, especially for relatively slow growing native species. Topsoil is generally removed during construction, and the surface soil following construction (“cut-slope soils”) is often compacted and low in nutrients, providing poor growing conditions for vegetation. Nebraska Department of Transportation (NDOT) protocols have historically called for nitrogen (N) and phosphorus (P) fertilization when planting roadside vegetation following construction, but these recommendations were developed for cool-season grass plantings and most current plantings use slower-establishing, native warmseason grasses that may benefit less than expected from current planting protocols. We evaluated the effects of nitrogen and …

Characterization Of A Novel Mitochondrial Plasmid In Brassica, Mackenzie Strehle

UCARE Research Products

Possessing some of the largest and most complex genomes of any eukaryotic organelles, plant mitochondria are notorious for their rapidly rearranging genetic framework. In addition to containing a large and complex mitochondrial genome, the mitochondria of several plants in the genus Brassica have also been shown to contain an independent, self-replicating linear plasmid. Interestingly, the plasmid appears to be able to move independently between the cytoplasm and the mitochondria, and it can be paternally inherited, unlike the rest of the mitochondrial genome. The plasmid also has features similar to those of adenoviruses, including terminal inverted repeats and covalently bound proteins …

Multiple Origins Of Endosymbionts In Chlorellaceae With No Reductive Effects On The Plastid Or Mitochondrial Genomes, Weishu Fan, Wenhu Guo, James L. Van Etten, Jeffrey P. Mower

James Van Etten Publications

Ancient endosymbiotic relationships have led to extreme genomic reduction in many bacterial and eukaryotic algal endosymbionts. Endosymbionts in more recent and/or facultative relationships can also experience genomic reduction to a lesser extent, but little is known about the effects of the endosymbiotic transition on the organellar genomes of eukaryotes. To understand how the endosymbiotic lifestyle has affected the organellar genomes of photosynthetic green algae, we generated the complete plastid genome (plastome) and mitochondrial genome (mitogenome) sequences from three green algal endosymbionts (Chlorella heliozoae, Chlorella variabilis and Micractinium conductrix). The mitogenomes and plastomes of the three newly sequenced endosymbionts …

Control Of Glyphosate-Resistant Common Ragweed (Ambrosia Artemisiifolia L.) In Glufosinate-Resistant Soybean [Glycine Max (L.) Merr], Ethann R. Barnes, Stevan Z. Knezevic, Peter H. Sikkema, John L. Lindquist, Amit J. Jhala

Department of Agronomy and Horticulture: Faculty Publications

Common ragweed emerges early in the season in Nebraska, USA and is competitive with soybean; therefore, preplant herbicides are important for effective control. Glyphosate has been used as a preplant control option; however, confirmation of glyphosate-resistant (GR) common ragweed in Nebraska necessitates evaluating other herbicide options. The objectives of this study were to (1) evaluate the efficacy of preplant (PP) herbicides followed by (fb) glufosinate alone or in tank-mixture with imazethapyr, acetochlor, or S-metolachlor applied post-emergence (POST) for control of GR common ragweed in glufosinate-resistant soybean; (2) their effect on common ragweed density, biomass, and soybean yield; and (3) the …

Improving Wildflower Longevity In Roadside Seeding Areas, Walter Schacht, Jonathan M. Soper, Carol Wienhold

Nebraska Department of Transportation: Research Reports

Re-vegetation efforts on bare roadsides of newly-constructed highways are primarily focused on the stabilization of soil to reduce rates of erosion. The Nebraska Department of Transportation (NDOT) seeds roadsides with a diverse mixture of grasses and wildflowers for site stabilization as well as to enhance the visual quality of roadsides. Although grasses dominate roadside plantings in terms of cover and density, wildflowers are largely responsible for the visual enhancement of recently-seeded roadsides. In addition to the visual component, wildflowers provide essential ecological functions on roadsides. Wildflowers improve water and nutrient cycling in the compacted roadside soils by increasing water infiltration …

Impact Of Seed Protein Alleles From Three Soybean Sources On Seed Composition And Agronomic Traits, Lillian F. Brzostowski, Timothy I. Pruski, James Specht, Brian W. Diers

Department of Agronomy and Horticulture: Faculty Publications

Evaluation of seed protein alleles in soybean populations showed that an increase in protein concentration is generally associated with a decrease in oil concentration and yield.

Soybean [Glycine max (L.) Merrill] meal is one of the most important plant-based protein sources in the world. Developing cultivars high in seed protein concentration and seed yield is a difficult task because the traits have an inverse relationship. Over two decades ago, a protein quantitative trait loci (QTL) was mapped on chromosome (chr) 20, and this QTL has been mapped to the same position in several studies and given the confirmed QTL …

Differentially Regulated Orthologs In Sorghum And The Subgenomes Of Maize, Yang Zhang, Daniel W. Ngu, Daniel Santana De Carvalho, Zhikai Liang, Yumou Qiu, Rebecca Roston, James C. Schnable

Center for Plant Science Innovation: Faculty and Staff Publications

Identifying interspecies changes in gene regulation, one of the two primary sources of phenotypic variation, is challenging on a genome-wide scale. The use of paired time-course data on cold-responsive gene expression in maize (Zea mays) and sorghum (Sorghum bicolor) allowed us to identify differentially regulated orthologs. While the majority of cold-responsive transcriptional regulation of conserved gene pairs is species specific, the initial transcriptional responses to cold appear to be more conserved than later responses. In maize, the promoters of genes with conserved transcriptional responses to cold tend to contain more micrococcal nuclease hypersensitive sites in their …

Msh1-Mediated Non-Genetic Phenotypic Variation In Plants: Prospects For Epigenetic Breeding In Sorghum Bicolor, Dikungwa Ketumile

Department of Agronomy and Horticulture: Dissertations, Theses, and Student Research

Sorghum is the fifth most important cereal crop grown in the world, and is the primary staple food for many people in developing countries. Like other major crops, its improvement is constrained by depletion of genetic diversity within breeding germplasm. Investigated in this study is the unique creation of non-genetic phenotypic variation through altering MutS Homolog1 (MSH1), a plant-specific gene, and the potential it presents for sorghum improvement. Suppression of MSH1 results in non-genetic developmental reprogramming. The derived MSH1 memory lines, when used in crossing, result in heritable phenotypic variation that enhances plant vigor and agronomic performance. A …