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Full-Text Articles in Life Sciences

Life History Of An Exotic Soft Scale Insect Phalacrococcus Howertoni (Hemiptera: Coccidae) Found In Florida, Kaushalya G. Amarasekare, Catharine M. Mannion Sep 2011

Life History Of An Exotic Soft Scale Insect Phalacrococcus Howertoni (Hemiptera: Coccidae) Found In Florida, Kaushalya G. Amarasekare, Catharine M. Mannion

Agricultural and Environmental Sciences Faculty Research

We investigated the life history of an exotic soft scale insect, Phalacrococcus howertoni Hodges and Hodgson that damages croton and several other economically important ornamental and fruit plants in Florida. There was no difference in the development, survival, and reproduction of this scale insect species when reared either on croton or on buttonwood at 27 ± 1°C, 12:12 (L:D) h and 65% R. H. The scale insect practiced ovoviviparity. We observed that eggs that came out from vulva hatched to first instars immediately. Newly hatched nymphs had a tendency stay underneath the female body for somewhat less than approximately 24 …


Identification Of Salt-Induced Changes In Leaf And Root Proteomes Of The Wild Tomato, Solanum Chilense, Suping Zhou, Roger J. Sauvé, Zong Liu, Sasikiran Reddy, Sarabjit Bhatti, Simon D. Hucko, Tara Fish, Theodore W. Thannhauser Jul 2011

Identification Of Salt-Induced Changes In Leaf And Root Proteomes Of The Wild Tomato, Solanum Chilense, Suping Zhou, Roger J. Sauvé, Zong Liu, Sasikiran Reddy, Sarabjit Bhatti, Simon D. Hucko, Tara Fish, Theodore W. Thannhauser

Agricultural and Environmental Sciences Faculty Research

This article reports salt-induced changes in leaf and root proteomes after wild tomato (Solanum chilense) plants were treated with 200 mmNaCl. In leaf tissues, a total of 176 protein spots showed significant changes (P < 0.05), of which 104 spots were induced and 72 spots suppressed. Salt-induced proteins are associated with the following pathways: photosynthesis, carbohydrate metabolism, glyoxylate shunt, glycine cleavage system, branched-chain amino acid biosynthesis, protein folding, defense and cellular protection, signal transduction, ion transport, and antioxidant activities. Suppressed proteins belong to the following categories: oxidative phosphorylation pathway, photorespiration and protein translational machinery, oxidative stress, and ATPases. In root tissues, 106 protein spots changed significantly (P< 0.05) after the salt treatment, 63 spots were induced, and 43 suppressed by salt treatment. Salt-induced proteins are associated with the following functional pathways: regeneration of S-adenosyl methionine, protein folding, selective ion transport, antioxidants and defense mechanism, signal transduction and gene expression regulation, and branched-chain amino acid synthesis. Salt-suppressed proteins are receptor kinase proteins, peroxidases and germin-like proteins, malate dehydrogenase, and glycine dehydrogenase. In this study, different members of proteins were identified from leaf and root tissues after plants were subjected to salt treatment. These proteins represent …


Identification Of Alternaria Alternata As A Causal Agent For Leaf Blight In Syringa Species, Margaret T. Mmbaga, Ainong Shi, Mee-Sook Kim Jun 2011

Identification Of Alternaria Alternata As A Causal Agent For Leaf Blight In Syringa Species, Margaret T. Mmbaga, Ainong Shi, Mee-Sook Kim

Agricultural and Environmental Sciences Faculty Research

While many isolates of Alternaria alternata are common saprophytes on trees and shrubs, this study clearly demonstrated that A. alternata is a primary pathogen in lilac (Syringa sp.), causing a leaf-blight that affects different Syringa species. Isolates of Alternaria sp. were collected from leaf blight samples of lilacs in the field. The internal transcribed spacer (ITS) region and morphological characterization were used to identify lilac blight pathogen. Based on 100% ITS nucleotide sequence identities to the Alternaria genus in the GenBank and morphological features, these isolates were identified as A. alternata. Disease symptoms were reproduced in lilac plants inoculated with …


Heat-Induced Proteome Changes In Tomato Leaves, Suping Zhou, Roger J. Sauvé, Zong Liu, Sasikiran Reddy, Sarabjit Bhatti, Simon D. Hucko, Yang Yong, Tara Fish, Theodore W. Thannhauser May 2011

Heat-Induced Proteome Changes In Tomato Leaves, Suping Zhou, Roger J. Sauvé, Zong Liu, Sasikiran Reddy, Sarabjit Bhatti, Simon D. Hucko, Yang Yong, Tara Fish, Theodore W. Thannhauser

Agricultural and Environmental Sciences Faculty Research

Three tomato (Solanum lycopersicum) cultivars [Walter LA3465 (heat-tolerant), Edkawi LA 2711 (unknown heat tolerance, salt-tolerant), and LA1310 (cherry tomato)] were compared for changes in leaf proteomes after heat treatment. Seedlings with four fully expanded leaves were subjected to heat treatment of 39/25 °C at a 16:8 h light–dark cycle for 7 days. Leaves were collected at 1200 hr, 4 h after the light cycle started. For ‘Walter’ LA3465, heat-suppressed proteins were geranylgeranyl reductase, ferredoxin-NADP (+) reductase, Rubisco activase, transketolase, phosphoglycerate kinase precursor, fructose–bisphosphate aldolase, glyoxisomal malate dehydrogenase, catalase, S-adenosyl-L-homocysteine hydrolase, and methionine synthase. Two enzymes were induced, cytosolic …


Prunus Domestica Pathogenesis-Related Protein-5 Activates The Defense Response Pathway And Enhances The Resistance To Fungal Infection, Ashraf El-Kereamy, Islam El-Sharkawy, Rengasamy Ramamoorthy, Ali Taheri, Deena Errampalli, Prakash Kumar, Subramanian Jayasankar Mar 2011

Prunus Domestica Pathogenesis-Related Protein-5 Activates The Defense Response Pathway And Enhances The Resistance To Fungal Infection, Ashraf El-Kereamy, Islam El-Sharkawy, Rengasamy Ramamoorthy, Ali Taheri, Deena Errampalli, Prakash Kumar, Subramanian Jayasankar

Agricultural and Environmental Sciences Faculty Research

Pathogenesis-related protein-5 (PR-5) has been implicated in plant disease resistance and its antifungal activity has been demonstrated in some fruit species. However, their roles, especially their interactions with the other defense responses in plant cells, are still not fully understood. In this study, we have cloned and characterized a new PR-5 cDNA named PdPR5-1 from the European plum (Prunus domestica). Expression of PdPR5-1 was studied in different cultivars varying in resistance to the brown rot disease caused by the necrotrophic fungus Monilinia fructicola. In addition transgenic Arabidopsis, ectopically expressing PdPR5-1 was used to study its role in other plant defense …