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Full-Text Articles in Life Sciences
Inhibition Of Cysteine And Aspartyl Proteinases In The Alfalfa Weevil Midgut With Biochemical And Plant-Derived Proteinase Inhibitors, Stephen E. Wilhite, Thomas C. Elden, Joze Brzin, Ann C. Smigocki
Inhibition Of Cysteine And Aspartyl Proteinases In The Alfalfa Weevil Midgut With Biochemical And Plant-Derived Proteinase Inhibitors, Stephen E. Wilhite, Thomas C. Elden, Joze Brzin, Ann C. Smigocki
United States Department of Agriculture-Agricultural Research Service / University of Nebraska-Lincoln: Faculty Publications
Proteolytic activities in alfalfa weevil (Hypera postica) larval midguts have been characterized. Effects of pH, thiol activators, low-molecular weight inhibitors, and proteinase inhibitors (PIs) on general substrate hydrolysis by midgut extracts were determined. Hemoglobinolytic activity was highest in the acidic to mildly acidic pH range, but was maximal at pH 3.5. Addition of thiolactivators dithiothreitol (DTT), 2-mercaptoethanol (2-ME), or l-cysteine had little effect on hemoglobin hydrolysis at pH 3.5, but enhanced azocaseinolytic activity two to three-fold at pH 5.0. The broad cysteine PI E-64 reduced azocaseinolytic activity by 64% or 42% at pH 5 in the presence or …
Autophagomyces, Bordea, And A New Genus, Rossiomyces, (Laboulbeniales), Richard K. Benjamin
Autophagomyces, Bordea, And A New Genus, Rossiomyces, (Laboulbeniales), Richard K. Benjamin
Aliso: A Journal of Systematic and Floristic Botany
A revision of Autophagomyces (Laboulbeniales) was made based on a comparative morphological study of new collections and 16 species included in the genus by Thaxter in the final volume of his monograph published in 1931 and by others more recently. The concept of Autophagomyces (species on Anthicidae, Phalacridae, and Scapidiidae [Coleoptera]) was narrowed and comprises 12 species, four of them new, i.e., A. hammondii, A. mexicanus, A. protuberans, and A. ramosus. The genus Bordea (species on Pselaphinae, Staphylinidae [Coleopteraj), which had been included in Autophagomyces by Thaxter, was reinstated and comprises 14 species, seven new, i.e., …