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Identification Of Dna Markers In Triticum Aestivum-Aegilops Caudata Additions Lines By Randomly Amplified Polymorphic Dna (Rapd) Technology, Ling Wei
All Graduate Theses and Dissertations, Spring 1920 to Summer 2023
The objective of this study was to identify DNA markers for each of six added C-genome chromosomes in Triticum aestivum L. cv. 'Alceso'-Aegilops caudata L. addition lines using the randomly amplified polymorphic DNA (RAPD) technique. DNA from Ae. caudata, T. aestivum, amphiploid of T. aestivum X Ae. caudata, and six disomic addition lines of wheat having a pair of Ae. caudata chromosomes was used as the template for the amplification of RAPD markers with a total of 58 random 10-mer oligonucleotide primers. Two primers, OPC-08 and OPJ-16, produced one intense band each from the amphiploid of …